ABI 3100 DNA Analyzer for Established Core Facility

适用于已建立的核心设施的 ABI 3100 DNA 分析仪

• 批准号: 6877429
• 负责人: THOMAS J KELLER
• 金额: $ 14.27万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877429
• 关键词: DNA; biomedical equipment purchase; biosensor device; nucleic acid sequence

DESCRIPTION (provided by applicant): Funds for an Applied Biosystems Inc. 3100 16 Capillary DNA Analyzer and manufacturer recommended accessories are requested. It will become the principle instrument for the DNA sequencing & analysis services provided by the MMI Research Core Facility (http://www.ohsu.edu/research/core). This well established core facility provides DNA sequencing services to more than 100 basic medical science researchers in >20 departments and institutes at the Oregon Health & Science University. It also provides DNA sequencing services to 7 local colleges and institutions in the Portland area. The progress of more than 175 NIH funded biomedical research projects will be improved by the acquisition of the requested instrument. Some specific examples include Brian Druker's work identifying new kinase targets in acute myeloid leukemia, D. Farrell's investigation of the regulation and function of human fibrinogen isoforms associated with coronary heart disease, F. Heffron's studies of bacterial pathogenicity islands, markers for susceptibility to autoimmune disease in D. Kabat's lab, G. Burrows' work in developing MHC class II constructs for autoimmune therapy, P. Stork's studies of the role of the small G protein, Rap1, in regulating the MAP kinase activation pathway in T lymphocytes, P. Rotwein's studies insulin-like growth factors and the differentiation of muscle, and G. Banker's studies on the membrane trafficking and the development of neuronal polarity. The increased throughput and improved turnaround time achieved with the 3100 will also contribute significantly to new projects needing high-throughput SNP and fragment analysis for genotyping. The core facility has a full-time PhD director, a full-time senior technician and two part time technicians. Data management systems are well established. Institutional support is provided by the Molecular Microbiology & Immunology Department, the Oregon Cancer Institute, and the School of Medicine and the OHSU Office of Research.

描述（由申请人提供）：申请应用生物系统公司310016毛细管DNA分析仪和制造商推荐配件的资金。它将成为MMI研究核心设施（http://www.ohsu.edu/research/core）提供的DNA测序和分析服务的主要仪器。这个完善的核心设施为俄勒冈健康与科学大学20多个部门和研究所的100多名基础医学研究人员提供DNA测序服务。它还为波特兰地区的7所当地大学和机构提供DNA测序服务。超过175个NIH资助的生物医学研究项目的进展将通过获得所要求的仪器而得到改善。一些具体的例子包括Brian Druker在急性髓性白血病中发现新的激酶靶点的工作，D. Farrell对与冠心病相关的人类纤维蛋白原亚型的调节和功能的研究，F. Heffron对细菌致病性岛的研究，D. Kabat实验室对自身免疫性疾病易感性标记的研究，G. Burrows在开发用于自身免疫性治疗的MHC II类构建物的工作，P. Stork对小G蛋白作用的研究，Rap1在T淋巴细胞中调控MAP激酶激活途径，P. Rotwein研究胰岛素样生长因子与肌肉分化，G. Banker研究膜转运与神经元极性的形成。3100增加的吞吐量和缩短的周转时间也将为需要高通量SNP和片段分析进行基因分型的新项目做出重大贡献。