ABI 3100 DNA Analyzer for Established Core Facility

描述（由申请人提供）：应用生物系统公司的资金。要求3100 16毛细管DNA分析仪和制造商推荐的附件。它将成为MMI研究核心设施（http：//www.ohsu.edu/research/core）提供的DNA测序和分析服务的主要仪器。这个完善的核心设施为俄勒冈州健康与科学大学20多个部门和研究所的100多名基础医学科学研究人员提供DNA测序服务。它还为波特兰地区的7所当地学院和机构提供DNA测序服务。超过175个NIH资助的生物医学研究项目的进展将通过获得所要求的仪器而得到改善。一些具体的例子包括布赖恩德鲁克的工作，确定新的激酶在急性髓细胞白血病，D。Farrell对与冠心病相关的人纤维蛋白原异构体的调节和功能的研究，F。Heffron对细菌致病岛的研究是D.卡巴特的实验室，G。Burrows在开发用于自身免疫治疗的MHC II类结构方面的工作，P.Stork对小G蛋白Rap1在调节T淋巴细胞中MAP激酶活化途径中的作用的研究，P.Rotwein对胰岛素样生长因子和肌肉分化的研究，以及G.班克关于膜运输和神经元极性发育的研究。3100型分析仪提高的通量和缩短的周转时间也将为需要高通量SNP和片段分析的基因分型新项目做出重大贡献。 核心设施有一名全职博士生主任，一名全职高级技术员和两名兼职技术员。数据管理系统已经建立。机构支持由分子微生物学和免疫学系，俄勒冈州癌症研究所，医学院和OHSU研究办公室提供。

DESCRIPTION (provided by applicant): Funds for an Applied Biosystems Inc. 3100 16 Capillary DNA Analyzer and manufacturer recommended accessories are requested. It will become the principle instrument for the DNA sequencing & analysis services provided by the MMI Research Core Facility (http://www.ohsu.edu/research/core). This well established core facility provides DNA sequencing services to more than 100 basic medical science researchers in >20 departments and institutes at the Oregon Health & Science University. It also provides DNA sequencing services to 7 local colleges and institutions in the Portland area. The progress of more than 175 NIH funded biomedical research projects will be improved by the acquisition of the requested instrument. Some specific examples include Brian Druker's work identifying new kinase targets in acute myeloid leukemia, D. Farrell's investigation of the regulation and function of human fibrinogen isoforms associated with coronary heart disease, F. Heffron's studies of bacterial pathogenicity islands, markers for susceptibility to autoimmune disease in D. Kabat's lab, G. Burrows' work in developing MHC class II constructs for autoimmune therapy, P. Stork's studies of the role of the small G protein, Rap1, in regulating the MAP kinase activation pathway in T lymphocytes, P. Rotwein's studies insulin-like growth factors and the differentiation of muscle, and G. Banker's studies on the membrane trafficking and the development of neuronal polarity. The increased throughput and improved turnaround time achieved with the 3100 will also contribute significantly to new projects needing high-throughput SNP and fragment analysis for genotyping. The core facility has a full-time PhD director, a full-time senior technician and two part time technicians. Data management systems are well established. Institutional support is provided by the Molecular Microbiology & Immunology Department, the Oregon Cancer Institute, and the School of Medicine and the OHSU Office of Research.