Molecular mechanisms of synapse lose by polo kinases

Polo 激酶导致突触丢失的分子机制

• 批准号: 7176204
• 负责人: Daniel T Pak
• 金额: $ 27.13万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7176204
• 关键词: Address; Affect; Binding; Biochemical; Biological Assay; COS Cells; Chromosome Pairing; Condition; Data; Dementia; Dendritic Spines; Development; Drosophila polo protein; Equilibrium; Event; Excision; Excitatory Synapse; Family; Growth; Hippocampus (Brain); Human; Impaired cognition; In Vitro; Knowledge; Learning; Life; Memory; Methods; Modeling; Molecular; Monomeric GTP-Binding Proteins; Morphology; Nerve Degeneration; Neurobiology; Neurons; Numbers; Pathway interactions; Peptides; Phosphorylation; Phosphorylation Site; Phosphotransferases; Play; Preparation; Procedures; Process; Property; Protein Overexpression; Protein-Serine-Threonine Kinases; Proteins; Public Health; RNA Interference; Regulation; Role; Screening procedure; Shapes; Signal Transduction; Signaling Molecule; Site; Structure; Synapses; Synaptic Potentials; Synaptic plasticity; Synaptosomes; Testing; Ubiquitin; Vertebral column; Viral; base; cell motility; clinically significant; density; insight; morphogens; neocortical; neurodegenerative dementia; novel; postsynaptic; research study; serum-inducible kinase; synaptogenesis

DESCRIPTION (provided by applicant): The proper development of neuronal connections depends on precisely controlling the balance between formation and elimination of synapses. These opposing processes also play important roles in synaptic plasticity, learning and memory. Compared to the current wealth of data regarding synaptogenesis, however, relatively little is known about the molecular events underlying the loss of central synapses. We have recently identified a novel mechanism of synapse loss centered on the serum-inducible kinase (SNK), a serine-threonine protein kinase of the polo family. In cortex and hippocampus, the short-lived SNK is induced by synaptic activity and subsequently promotes loss of excitatory synapses and dendritic spines (the primary loci of excitatory synapses in the CMS). SNK exerts its effect on synapses via the phosphorylation- and ubiquitin-dependent degradation of specific postsynaptic substrates such as SPAR, an important morphogen for dendritic spines. Because SNK regulates the number, structure and composition of synapses and spines, this kinase is likely to be important for shaping the long-term functional properties of neurons. We have identified an additional potential substrate of SNK, the abundant postsynaptic Ras regulator SynGAP. Ras signaling is of critical importance for a wide variety of neurobiological processes including synaptic plasticity, development, and protection from excitotoxic insults. Biochemical and molecular approaches will be employed in Aim 1 to determine whether SynGAP and SNK physically interact and whether SynGAP is a direct phosphorylation substrate of SNK. In Aim 2 we will analyze the functional relationship between SNK and SynGAP in vitro and determine whether SNK regulates SynGAP and Ras in neurons. Finally, the role of a putative SNK/SynGAP/Ras regulatory network in regulating dendritic spine morphology will be addressed in Aim 3. The experiments described in this proposal are essential for understanding the mechanisms of SNK action and may have broad impact in the fields of synaptic plasticity, synapse development, and pathological synapse loss. Elucidating these pathways is likely to be of clinical significance and highly relevant to public health in view of the fact that neocortical synapse loss is a hallmark of human neurodegeneration and is the major correlate of cognitive decline in many forms of dementia.

描述（由申请人提供）：神经元连接的正确发育取决于精确控制突触形成和消除之间的平衡。这些相反的过程在突触可塑性、学习和记忆中也发挥着重要作用。然而，与目前有关突触发生的大量数据相比，人们对中央突触丧失背后的分子事件知之甚少。我们最近发现了一种以血清诱导激酶（SNK）为中心的突触丢失的新机制，SNK是一种polo家族的丝氨酸-苏氨酸蛋白激酶。在皮层和海马体中，短暂的 SNK 由突触活动诱导，随后促进兴奋性突触和树突棘（CMS 中兴奋性突触的主要位点）的丧失。 SNK 通过特定突触后底物（例如树突棘的重要形态发生素 SPAR）的磷酸化和泛素依赖性降解来对突触发挥作用。由于 SNK 调节突触和棘的数量、结构和组成，因此这种激酶可能对于塑造神经元的长期功能特性很重要。我们已经确定了 SNK 的另一个潜在底物，即丰富的突触后 Ras 调节因子 SynGAP。 Ras 信号传导对于多种神经生物学过程至关重要，包括突触可塑性、发育和免受兴奋性毒性损伤的保护。目标 1 将采用生化和分子方法来确定 SynGAP 和 SNK 是否发生物理相互作用以及 SynGAP 是否是 SNK 的直接磷酸化底物。在目标 2 中，我们将在体外分析 SNK 和 SynGAP 之间的功能关系，并确定 SNK 是否调节神经元中的 SynGAP 和 Ras。最后，目标 3 将讨论假定的 SNK/SynGAP/Ras 调节网络在调节树突棘形态中的作用。本提案中描述的实验对于理解 SNK 作用机制至关重要，并且可能在突触可塑性、突触发育和病理性突触损失领域产生广泛影响。鉴于新皮质突触丧失是人类神经退行性变的标志，并且是许多形式的痴呆症认知能力下降的主要相关因素，阐明这些途径可能具有临床意义，并且与公共健康高度相关。