Neural Development and Genetics of Zebrafish

描述(由申请人提供)： 这项提议要求部分支持为高级研究生、博士后研究员和独立研究人员举办的为期两周的强化讲座/实验室课程，重点是斑马鱼的发育和遗传学，并特别强调神经系统。课程的第一周涵盖斑马鱼的早期发育和遗传学以及相关的方法学，而第二周的课程重点是神经系统。上午和下午一般用于背景讲座和实验室练习，晚上用于研究讲座。每天在一名资深教员的监督下，由一名或两名初级教员协助。 在第一周，学生学习基本技术，如RNA和反义吗啡注射，转基因，镶嵌分析，以及整体RNA和抗体原位杂交，这些都是斑马鱼实验室的标准。第一周的第二个主要焦点是斑马鱼遗传学，内容包括诱变、遗传筛选方法和定位克隆。课程第二周的重点是斑马鱼神经系统的发育和功能，尽管许多技术也适用于研究其他器官系统的发育。例如，教授学生注射、标记和移植单细胞的各种技术，以评估基因功能、细胞命运或细胞在后期发育阶段的行为。课程的最后几天教授在基因和神经回路水平上研究复杂行为的技术。在实验室课程中，穿插着教授斑马鱼研究的实用方面的“圆桌”课程，例如如何建造和维护斑马鱼设施，如何繁殖斑马鱼，以及如何将斑马鱼从卵饲养到成鱼。

DESCRIPTION (provided by applicant): This proposal requests partial support for an intensive two-week lecture/laboratory course for advanced graduate students, postdoctoral fellows and independent investigators that focuses on the development and genetics of zebrafish with special emphasis on the nervous system. The first week of the course covers the early development and genetics of zebrafish and the relevant methodologies, whereas the second week focuses on the nervous system. Mornings and afternoons are generally devoted to background lectures and laboratory exercises and evenings to research lectures. Each day is under the supervision of a senior faculty member who is assisted by one or two junior faculty members. In the first week students learn basic techniques of RNA and antisense morpholino injections, transgenesis, mosaic analysis, and whole-mount RNA and antibody in situ hybridizations, standard to any zebrafish laboratory. A second major focus of the first week is zebrafish genetics, with sessions on mutagenesis, genetic screening methodologies and positional cloning. The second week of the course focuses on zebrafish nervous system development and function, although many of the techniques are applicable to studying the development of other organ systems. For example, students are taught a variety of techniques for injecting, labeling and transplanting single cells, in order to assess gene function, cell fate, or cell behaviors during later developmental stages. The final days of the course teach techniques for studying complex behaviors, both at the levels of genes and of neural circuits. Interspersed with the laboratory sessions, are "round table" sessions that teach the practical side of zebrafish research, such as how to build and maintain a zebrafish facility, how to breed zebrafish, and how to raise them from egg to adult.