Investigating the Assembly of Bacterial Encapsulins for the Efficient Engineering of Nanoscale Bioreactors

在这个博士项目中，巴林格先生将使用已建立的模块化合成生物学平台生产一系列天然和异源封装系统。与华莱士实验室合作，将选择一系列工业生物催化靶酶进行包封，并确定包封对稳定性和活性的影响。这些封装系统的组装，然后将使用几种结构质谱法的组合进行监测。天然质谱和离子迁移质谱技术允许观察和表征完整的生物素系统（3）。通过将这种技术与克拉克实验室开发的快速混合技术相结合（4），我们将在逐步组装中观察混合。这些研究将概述这些系统中蛋白质的有效包封的参数，并提供一个框架，以产生设计的包封酶。

In this PhD project Mr Ballinger will produce a series of native and heterologous encapsulated systems using an established modular synthetic biology platform. In collaboration with the Wallace Lab, a series of industrial biocatalytic target enzymes will be chosen for encapsulation and the effect of encapsulation on stability and activity will be determined. The assembly of these encapsulated systems will then be monitored using a combination of several structural mass spectrometry approaches. Native mass spectrometry and ion mobility mass spectrometry techniques allow the observation and characterisation of intact encapsulin systems (3). By combining this technology with rapid mixing techniques, developed in the Clarke Lab (4), we will observe encapsulin stepwise assembly. These studies will outline the parameters for efficient encapsulation of proteins in these systems and providing a framework to produce designed encapsulated enzymes.