CONTINUED HIGH RESOLUTION STRUCTURAL ANALYSIS OF APPA

APPA 的持续高分辨率结构分析

• 批准号: 7181920
• 负责人: CARL Eugene BAUER
• 金额: $ 1.01万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181920
• 关键词: biomedical resource; structural biology

DESCRIPTION (provided by applicant): We will continue to operate BioCARS, a nationally-available research resource for synchrotron-based macromolecular crystallography at the Advanced Photon Source, Argonne National Laboratory. The facility contains three experimental stations, one served by either an undulator or a wiggler insertion device source and two served by a bending magnet source, all of which can be used simultaneously. All stations and the associated control areas are embedded in a BL3-level biosafety facility, to permit the safe study of, for example, pathogenic human viruses. Research by users has successfully and productively concentrated on de novo structure determination by MAD phasing; on the collection of accurate, high resolution, monochromatic data; on the study of crystals with large unit cell dimensions; and on time-resolved crystallography on time scales down to a few nanoseconds. We will continue to emphasize these areas and to support the most challenging user experiments, through our experienced technical and scientific staff. In a facility that is unique nationally and one of three internationally, we will further develop time-resolved crystallography in both core and collaborative research, largely on the insertion device station. Advances in this scientific technique have led to much broader user interest in applying it to a wide variety of systems, since it offers new insights into biological mechanism and function. Upgrades will be made to the X-ray beam lines, both to replace those older components damaged by radiation and by heating and to afford smaller focal spots that are better matched to the smaller crystals that users wish to examine. Detector and data acquisition and control software will be improved, both to support collaborative projects in structural genomics that demand high data throughput, and to enable telecrystallography, in which the user can participate effectively in all aspects of the synchrotron experiment but need not be physically present at BioCARS. Strategies for improving the diffraction quality of crystals at both room and cryogenic temperatures by annealing will be implemented. In all cases, these developments are aimed at enhancing the throughput of excellent, high-resolution diffraction data and solved scientific problems, by satisfied users.

描述(申请人提供)：我们将继续运营BioCARS，这是阿贡国家实验室先进光子源的基于同步加速器的高分子结晶学的全国可用的研究资源。该设施包括三个实验站，一个由波荡器或摇摆器插入装置源提供服务，两个由弯曲磁源提供服务，所有这些都可以同时使用。所有空间站和相关的控制区都嵌入了BL3级生物安全设施，以允许对例如致病的人类病毒进行安全研究。 用户的研究已经成功地和富有成效地集中在通过MAD相移确定从头结构；收集准确的、高分辨率的单色数据；研究具有大的晶胞尺寸的晶体；以及在时间尺度上的时间分辨结晶学，精确到几纳秒。我们将继续强调这些领域，并通过我们经验丰富的技术和科学工作人员支持最具挑战性的用户实验。在一个在全国独一无二、在国际上是三个之一的设施中，我们将在核心和合作研究中进一步开发时间分辨结晶学，主要是在插入设备站上。这项科学技术的进步导致了更广泛的用户将其应用于各种系统的兴趣，因为它为生物机制和功能提供了新的见解。 将对X射线光束线进行升级，以更换那些因辐射和加热而损坏的旧部件，并提供与用户希望检查的较小晶体更匹配的较小焦点。将改进探测器、数据采集和控制软件，以支持结构基因组学中需要高数据吞吐量的合作项目，并使用户能够有效地参与同步加速器实验的所有方面，但不需要亲自出现在BioCARS。将实施通过退火来提高晶体在室温和低温下的衍射质量的策略。在所有情况下，这些发展的目的都是为了提高优秀的、高分辨率的衍射数据的吞吐量，并由满意的用户解决科学问题。