CONTINUED HIGH RESOLUTION STRUCTURAL ANALYSIS OF APPA

描述（由申请人提供）：我们将继续运营BioCARS，这是阿贡国家实验室高级光子源的同步加速器大分子晶体学的国家可用研究资源。该设施包括三个实验站，一个由波荡器或摇摆器插入装置源提供服务，两个由弯曲磁铁源提供服务，所有这些都可以同时使用。所有监测站和相关的控制区都设在BL 3级生物安全设施内，以便能够安全地研究例如人类致病病毒。 用户的研究已经成功和富有成效地集中在通过MAD定相进行从头结构测定;收集准确、高分辨率、单色数据;研究具有大晶胞尺寸的晶体;以及在时间尺度下降到几纳秒的时间分辨晶体学。我们将继续强调这些领域，并通过我们经验丰富的技术和科学人员支持最具挑战性的用户实验。在一个全国独一无二的设施和三个国际之一，我们将进一步发展时间分辨晶体学的核心和合作研究，主要是在插入设备站。这一科学技术的进步已经引起了更广泛的用户兴趣，将其应用于各种各样的系统，因为它提供了对生物机制和功能的新见解。 将对X射线束线进行升级，以取代那些因辐射和加热而损坏的旧部件，并提供更小的焦点，以便与用户希望检查的较小晶体更好地匹配。探测器和数据采集和控制软件将得到改进，以支持结构基因组学中需要高数据吞吐量的合作项目，并实现远程电子照相术，用户可以有效地参与同步加速器实验的各个方面，但不需要亲自出席BioCARS。将实施通过退火在室温和低温下提高晶体衍射质量的策略。在所有情况下，这些发展的目的是提高优秀的，高分辨率的衍射数据和解决科学问题的吞吐量，由满意的用户。

DESCRIPTION (provided by applicant): We will continue to operate BioCARS, a nationally-available research resource for synchrotron-based macromolecular crystallography at the Advanced Photon Source, Argonne National Laboratory. The facility contains three experimental stations, one served by either an undulator or a wiggler insertion device source and two served by a bending magnet source, all of which can be used simultaneously. All stations and the associated control areas are embedded in a BL3-level biosafety facility, to permit the safe study of, for example, pathogenic human viruses. Research by users has successfully and productively concentrated on de novo structure determination by MAD phasing; on the collection of accurate, high resolution, monochromatic data; on the study of crystals with large unit cell dimensions; and on time-resolved crystallography on time scales down to a few nanoseconds. We will continue to emphasize these areas and to support the most challenging user experiments, through our experienced technical and scientific staff. In a facility that is unique nationally and one of three internationally, we will further develop time-resolved crystallography in both core and collaborative research, largely on the insertion device station. Advances in this scientific technique have led to much broader user interest in applying it to a wide variety of systems, since it offers new insights into biological mechanism and function. Upgrades will be made to the X-ray beam lines, both to replace those older components damaged by radiation and by heating and to afford smaller focal spots that are better matched to the smaller crystals that users wish to examine. Detector and data acquisition and control software will be improved, both to support collaborative projects in structural genomics that demand high data throughput, and to enable telecrystallography, in which the user can participate effectively in all aspects of the synchrotron experiment but need not be physically present at BioCARS. Strategies for improving the diffraction quality of crystals at both room and cryogenic temperatures by annealing will be implemented. In all cases, these developments are aimed at enhancing the throughput of excellent, high-resolution diffraction data and solved scientific problems, by satisfied users.