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Swellable Templated Copolymers

可溶胀模板共聚物

基本信息

批准号：
7075605
负责人：
Rudolf W. Seitz
金额：
$ 21.48万
依托单位：
UNIVERSITY OF NEW HAMPSHIRE
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-01 至 2010-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We have prepared a swellable templated copolymer that selectively recognizes theophylline in the presence of caffeine, a compound that differs from theophylline by a single methyl group. The copolymer includes 10 to 20 mole-% recognition monomer and 5 mole-% crosslinker. The rest is N-isopropylacrylamide or n-propylacrylamide. This polymer undergoes a thermal phase transition, coming out of solution above the thermal phase transition temperature. Molecular recognition occurs above the thermal phase transition. We have demonstrated response to theophylline below 0.1 micromolar at room temperature and neutral pH. These copolymers are stable and easily prepared in large quantities. They offer new possibilites for designing sensors and actuators for biomedical applications. They can be used for continuous sensing of drugs or endogenous compounds. They can be employed as actuators in drug delivery systems. They can potentially be used for chemically triggered delivery of trapped compounds. This application has two specific aims. The first specific aim is to synthesize a copolymer that selectively recognizes glutamine and to incorporate this material into a sensor that is capable of in situ monitoring of glutamine in fermentation broths. Two sensing schemes are proposed. One involves measuring changes in the turbidity of membranes consisting of copolymer microspheres embedded in a hydrogel. Turbidity changes because swelling and shrinking changes copolymer refractive index. The turbidity can be measured remotely through optical fibers using components developed for optical telecommunications. The second involves incorporating donor and acceptor fluorophores into the copolymer at levels such that swelling and shrinking change the extent of fluorescence resonance energy transfer, leading to a change in the ratio of donor to acceptor fluorescence. The second is to demonstrate the feasibility of chemically triggered delivery of trapped target molecules under physiological conditions. The trigger molecule will be theophylline. The rate of release of fluorescent target molecules will be monitored as a function of time for different release conditions.

描述（由申请人提供）：我们已经制备了一种可溶胀的模板化共聚物，其在咖啡因的存在下选择性地识别茶碱，咖啡因是一种与茶碱不同的单一甲基的化合物。该共聚物包括10至20摩尔%的识别单体和5摩尔%的交联剂。其余为N-异丙基丙烯酰胺或N-丙基丙烯酰胺。该聚合物经历热相变，在热相变温度以上从溶液中出来。分子识别发生在热相变之上。我们已经证明了响应茶碱低于0.1微摩尔在室温和中性pH值。这些共聚物是稳定的，易于大量制备。它们为设计生物医学应用的传感器和执行器提供了新的可能性。它们可用于药物或内源性化合物的连续传感。它们可以用作药物输送系统中的致动器。它们可以潜在地用于捕获化合物的化学触发递送。这项申请有两个具体目标。第一个具体目标是合成一种共聚物，选择性地识别谷氨酰胺，并将这种材料纳入传感器，能够在发酵液中的谷氨酰胺原位监测。提出了两种传感方案。一种涉及测量由嵌入水凝胶中的共聚物微球组成的膜的浊度变化。浊度的变化是因为溶胀和收缩改变了共聚物的折射率。浊度可以通过光纤使用为光通信开发的组件进行远程测量。第二种方法涉及将供体和受体荧光团以一定水平掺入共聚物中，使得溶胀和收缩改变荧光共振能量转移的程度，导致供体与受体荧光的比率改变。第二是证明在生理条件下化学触发递送捕获的靶分子的可行性。触发分子是茶碱。对于不同的释放条件，荧光靶分子的释放速率将作为时间的函数进行监测。