CORE--Molecular Resources Core

CORE--分子资源核心

• 批准号: 7367825
• 负责人: Randal A Skidgel
• 金额: $ 27.76万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367825
• 关键词: Endothelial Cells; Generations; Methods; Molecular; Postdoctoral Fellow; Quality Control; Reagent; Research; Resources; Services; Site; Small Interfering RNA; Specialist; Speed; TAF8 gene; Time; base; design; desire; expression vector; mutant; programs

Core C, the Molecular Resources Core, will provide molecular reagents and services to all four projects and will be crucial to the completion of the specific aims of each proposal in the Program Project. The proposal for a Molecular Resources Core is a continuation of the original Core C. The overall function of Core C is to provide molecular reagents and expertise, including generation of deletion and site-directed mutants and incorporation into the desired expression vectors, creation of minigene constructs, generation of adenoviral and retroviral constructs for transduction of primary endothelial cells and designing and generating appropriate siRNA based constructs. This core will be directed by Dr. Randal A. Skidgel and will be staffed by a full-time Senior Research Specialist (Dr. Barbara Keith), full- time Research Specialist (TBN) and part-time Research Associate (Vidas Dumasias). Consolidating these efforts in Core C will result in increased speed and efficiency in accomplishing the research tasks outlined in each of the projects and uniformity of approaches and methods so that resutts from each project can be compared. In addition, it will allow strict quality control and consistency of molecular reagents to be used by the various projects. Furthermore, an added and important benefit of the centralized Core C will be to markedly decrease expenses compared to a scenario in which each project would generate its own molecular resources. Regular meetings of the Core leader with the Senior Research Specialist and project leaders will assure coordination and prioritization of the generation of molecular reagents and assure that the desired reagents are provided in a timely manner to the component projects.

核心C，即分子资源核心，将为所有四个项目提供分子试剂和服务，对完成计划项目中每个提案的具体目标至关重要。分子资源核心的提议是原始核心C的延续。核心C的总体功能是提供分子试剂和专业知识，包括产生缺失和定点突变体并掺入所需的表达载体中，产生小基因构建体，产生用于转导原代内皮细胞的腺病毒和逆转录病毒构建体，以及设计和产生适当的基于siRNA的构建体。该核心将由Randal A博士指导。Skidgel将配备一名全职高级研究专家（Barbara基思博士）、全职研究专家（TBN）和兼职研究助理（Vidas Dumasias）。在核心C中巩固这些努力将提高完成每项研究任务的速度和效率， 项目和统一的途径和方法，使每个项目的结果可以比较。此外，它将允许严格的质量控制和各项目所用分子试剂的一致性。此外，集中式核心C的一个额外的重要好处是，与每个项目都产生自己的分子资源的情况相比，将显着减少费用。核心负责人与高级研究专家和项目负责人的定期会议将确保分子试剂生成的协调和优先顺序，并确保及时向组成项目提供所需试剂。