CORE--Molecular Resources Core

CORE--分子资源核心

• 批准号: 7312504
• 负责人: Randal A Skidgel
• 金额: $ 27.47万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7312504
• 关键词: biochemistry; biomedical facility; genetic library; molecular genetics; nucleic acid sequence; peptide chemical synthesis; protein sequence; site directed mutagenesis

Core C, the Molecular Resources Core, will provide molecular reagents and services to all four projects and will be crucial to the completion of the specific aims of each proposal in the Program Project. The proposal for a Molecular Resources Core is a continuation of the original Core C. The overall function of Core C is to provide molecular reagents and expertise, including generation of deletion and site-directed mutants and incorporation into the desired expression vectors, creation of minigene constructs, generation of adenoviral and retroviral constructs for transduction of primary endothelial cells and designing and generating appropriate siRNA based constructs. This core will be directed by Dr. Randal A. Skidgel and will be staffed by a full-time Senior Research Specialist (Dr. Barbara Keith), full- time Research Specialist (TBN) and part-time Research Associate (Vidas Dumasias). Consolidating these efforts in Core C will result in increased speed and efficiency in accomplishing the research tasks outlined in each of the projects and uniformity of approaches and methods so that resutts from each project can be compared. In addition, it will allow strict quality control and consistency of molecular reagents to be used by the various projects. Furthermore, an added and important benefit of the centralized Core C will be to markedly decrease expenses compared to a scenario in which each project would generate its own molecular resources. Regular meetings of the Core leader with the Senior Research Specialist and project leaders will assure coordination and prioritization of the generation of molecular reagents and assure that the desired reagents are provided in a timely manner to the component projects.

核心C，即分子资源核心，将为所有四个项目提供分子试剂和服务，并将对完成计划项目中每个提案的具体目标至关重要。Core的总体功能是提供分子试剂和专业知识，包括产生缺失和定点突变体，并将其整合到所需的表达载体中，创建minigene构建体，生成用于原代内皮细胞转导的腺病毒和逆转录病毒构建体，以及设计和生成适当的基于siRNA的构建体。该核心将由Randal a . Skidgel博士指导，并将由全职高级研究专家（Barbara Keith博士），全职研究专家（TBN）和兼职研究助理（Vidas Dumasias）组成。将这些努力整合到核心C中，将提高完成每一份报告中概述的研究任务的速度和效率