Shared Spinning Disk Confocal Microscope System

共享转盘共焦显微镜系统

• 批准号: 7046616
• 负责人: STEPHEN J DOXSEY
• 金额: $ 35.45万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046616
• 关键词: cell biology; cell cycle; community; digital imaging; molecular /cellular imaging; neoplastic cell; polycystic kidney; schools; syndrome; university

DESCRIPTION (provided by applicant): The Digital Imaging Core Facility (DICF) of the University of Massachusetts (UMass) Medical School and a group of seven NIH funded principal investigators request funds to purchase a spinning disk confocal microscope system for live cell imaging. The Digital Imaging Core Facility was established by the UMass Medical School Office of Research and Scientific Council to provide multimode digital imaging microscopy facilities and digital deconvolution to the UMass research community. The requested instrument will be integrated into this campus wide research resource and would be available to the entire UMass Medical School research community when it is not being used by the major and minor users. There is a great need by many NIH funded UMass Medical School researchers for a confocal microscope system designed for live cell imaging in order to accomplish the objectives of their NIH funded research. All of the researchers requesting this instrument are involved in qualitative and quantitative studies of dynamic cellular processes, and four are members of the UMass Cellular Dynamics Program. There is currently no spinning disk confocal microscope system for live cell imaging at UMass Medical School available for general use as part of a core facility. This spinning disk confocal microscope system will allow UMass researchers to expand their research in new directions and advance their biomedical research programs. The requested spinning disk confocal microscope system will be used to conduct basic research in cancer cell biology, cell signaling, developmental biology and cancer, cell cycle regulation, intraflagellar transport as related to polycystic kidney disease and retinitis pigmentosa, Bardet-Biedl syndrome and Senior-Loken syndrome, molecular motor proteins, sensory transduction and immunology. The Digital Imaging Light Microscopy Core Facility of the University of Massachusetts (UMass) Medical School and a group of seven UMass faculty members request funds to purchase a spinning disk confocal microscope system to advance their NIH funded biomedical research. The requested instrument will be used to conduct basic research in cancer cell biology, cell signaling, developmental biology and cancer, cell cycle regulation and intraflagellar transport as related to polycystic kidney disease and other diseases.

描述(由申请人提供)：马萨诸塞大学医学院的数字成像核心设施(DICF)和由七名NIH资助的主要研究人员组成的小组申请资金，购买用于活细胞成像的旋转圆盘共聚焦显微镜系统。数字成像核心设施是由华盛顿大学马萨诸塞州医学院研究和科学理事会办公室建立的，目的是向加州大学马萨诸塞州大学研究界提供多模式数字成像显微镜设施和数字反卷积。所要求的仪器将被整合到这个校园范围的研究资源中，当主要用户和次要用户不使用它时，整个UMAS医学院研究社区都可以使用它。许多美国国立卫生研究院资助的麻省理工学院医学院的研究人员非常需要一种用于活细胞成像的共焦显微镜系统，以实现他们资助的研究的目标。所有需要这台仪器的研究人员都参与了动态细胞过程的定性和定量研究，其中四人是加州大学马萨诸塞州大学细胞动力学计划的成员。目前，加州大学马萨诸塞州医学院没有用于活细胞成像的旋转圆盘共聚焦显微镜系统，可作为核心设施的一部分普遍使用。这一旋转圆盘共聚焦显微镜系统将允许马萨诸塞州大学的研究人员在新的方向上扩展他们的研究，并推进他们的生物医学研究计划。所要求的旋转圆盘共聚焦显微镜系统将用于进行癌症细胞生物学、细胞信号、发育生物学和癌症、细胞周期调节、与多囊肾病和视网膜色素变性、Bardet-Biedl综合征和High-Loken综合征有关的鞭毛内运输、分子马达蛋白、感觉转导和免疫学的基础研究。马萨诸塞大学医学院的数字成像光学显微镜核心设施和一个由七名马萨诸塞大学教职员工组成的小组申请资金购买旋转圆盘共焦显微镜系统，以推进他们由NIH资助的生物医学研究。所要求的仪器将用于进行与多囊肾病和其他疾病相关的癌细胞生物学、细胞信号、发育生物学和癌症、细胞周期调节和鞭毛内转运的基础研究。