SHARED SPINNING DISK CONFOCAL MICROSCOPE SYSTEM: CANCER

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：马萨诸塞州大学（UMass）医学院的数字成像核心设施（DICF）和一组由七名NIH资助的主要研究人员申请资金购买用于活细胞成像的旋转圆盘共聚焦显微镜系统。数字成像核心设施由马萨诸塞大学医学院研究和科学理事会办公室建立，为马萨诸塞大学研究界提供多模式数字成像显微镜设施和数字解卷积。所要求的仪器将被集成到这个校园范围内的研究资源，并将提供给整个马萨诸塞大学医学院的研究社区时，它不是由主要和次要用户使用。许多NIH资助的UMass医学院研究人员非常需要为活细胞成像设计的共聚焦显微镜系统，以实现NIH资助的研究目标。所有要求使用该仪器的研究人员都参与了动态细胞过程的定性和定量研究，其中四人是麻省大学细胞动力学计划的成员。目前没有旋转盘共聚焦显微镜系统的活细胞成像在马萨诸塞大学医学院可用于一般用途的核心设施的一部分。这种旋转圆盘共聚焦显微镜系统将使马萨诸塞大学的研究人员能够在新的方向上扩展他们的研究，并推进他们的生物医学研究计划。所要求的旋转圆盘共聚焦显微镜系统将用于在癌细胞生物学、细胞信号传导、发育生物学和癌症、细胞周期调节、与多囊肾病和色素性视网膜炎、Bardet-Biedl综合征和Senior-Loken综合征相关的鞭毛内转运、分子马达蛋白、感觉转导和免疫学方面进行基础研究。马萨诸塞州大学（UMass）医学院的数字成像光学显微镜核心设施和七名UMass教职员工申请资金购买旋转圆盘共聚焦显微镜系统，以推进NIH资助的生物医学研究。所要求的仪器将用于进行癌细胞生物学、细胞信号传导、发育生物学和癌症、细胞周期调节和与多囊肾病和其他疾病有关的鞭毛内运输等方面的基础研究。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Digital Imaging Core Facility (DICF) of the University of Massachusetts (UMass) Medical School and a group of seven NIH funded principal investigators request funds to purchase a spinning disk confocal microscope system for live cell imaging. The Digital Imaging Core Facility was established by the UMass Medical School Office of Research and Scientific Council to provide multimode digital imaging microscopy facilities and digital deconvolution to the UMass research community. The requested instrument will be integrated into this campus wide research resource and would be available to the entire UMass Medical School research community when it is not being used by the major and minor users. There is a great need by many NIH funded UMass Medical School researchers for a confocal microscope system designed for live cell imaging in order to accomplish the objectives of their NIH funded research. All of the researchers requesting this instrument are involved in qualitative and quantitative studies of dynamic cellular processes, and four are members of the UMass Cellular Dynamics Program. There is currently no spinning disk confocal microscope system for live cell imaging at UMass Medical School available for general use as part of a core facility. This spinning disk confocal microscope system will allow UMass researchers to expand their research in new directions and advance their biomedical research programs. The requested spinning disk confocal microscope system will be used to conduct basic research in cancer cell biology, cell signaling, developmental biology and cancer, cell cycle regulation, intraflagellar transport as related to polycystic kidney disease and retinitis pigmentosa, Bardet-Biedl syndrome and Senior-Loken syndrome, molecular motor proteins, sensory transduction and immunology. The Digital Imaging Light Microscopy Core Facility of the University of Massachusetts (UMass) Medical School and a group of seven UMass faculty members request funds to purchase a spinning disk confocal microscope system to advance their NIH funded biomedical research. The requested instrument will be used to conduct basic research in cancer cell biology, cell signaling, developmental biology and cancer, cell cycle regulation and intraflagellar transport as related to polycystic kidney disease and other diseases.