SMALL LAB ANIMAL IMAGING: LEUKEMIA, LUNG CANCER

小实验动物成像：白血病、肺癌

• 批准号: 7334992
• 负责人: RAMIRO Eliseo TORIBIO
• 金额: $ 6.87万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334992
• 关键词: X ray; androgens; animal tissue; biomaterials; birth; bone; bone development; bone neoplasms; breast neoplasms; cell biology; cell cycle; charge coupled device camera; complement; computers; disease /disorder model; gait; genes; growth factor; income; lead; leukemia; lung; measurement; metastasis; molecular /cellular imaging; neoplasm /cancer; osteoblasts; osteoclasts; prostate; skull; tissue engineering; tissues; tomography; training; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A High Resolution Microcomputed Tomography System, MicroCAT II, manufactured CTI Molecular Imaging, Inc. (Knoxville, TN) is being requested in this Shared Instrumentation Grant. The MicroCAT Imaging System was developed by the Department of Energy's Oak Ridge National Laboratories with the objective of accelerating the screening of genetically- engineered mice. The system consists of a microfocus X-ray source with a <10¿m focal spot size and larger focal spots for high-speed studies. For X-ray detection, the MicroCAT II has a high resolution 16 megapixel CCD camera with a large field of view (FOV) for screening and small FOV for high resolution imaging. The system is lead-shielded and FDA X-ray compliant. Specifications in the requested MicroCAT II system will allow NIH-funded researchers to image small laboratory animals and tissue specimens, fitting the investigators imaging needs. The MicroCAT II system bundled with the Amira Software will allow the non-invasive capture of data, generation of morphological information, longitudinal studies using landmark-based image registration applications, and high resolution 3D reconstructions necessary for disease modeling. There are no Micro-CT imaging systems at the Ohio State University. We have alternate methods to image laboratory animals and tissue specimens, however, these modalities are less sensitive for structural analysis, are time consuming, expensive to be used on a routine basis, have different applications, and do not allow longitudinal studies. A microCT system will maximize the generation of high quality information and will make the use of available resources more efficient and productive. This system will also complement the existing small animal imaging technologies. The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. The MicroCAT II system will be important for in vivo longitudinal studies, avoiding the early sacrifice of valuable research animals. It will also be useful to other researchers because it can be used to image biomaterials and soft tissue interphases. This system will increase the speed and ability to detect differences not possible before, thus increasing statistical power, making possible the evaluation of a larger number of animals, and allowing in vivo repeated measurements. The instrument will be located in a readily accessible biosecured animal room (Sisson Hall) of the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (specialized in imaging) and a Laboratory Animal Veterinarian. An Organizational and Administrative Plan for the placement, maintenance, and operation of the MicroCAT II Imaging System has been formulated. An hourly fee will provide funds for a service contract and continuing education for the supervisor. Billing will be performed by the Imaging Service of the Department of Veterinary Biosciences. The principal investigator has received trained on the use of the MicroCAT II system at Oak Ridge National Labs and at ImTek, Inc. (Knoxville, TN). Users will receive training by the system manufacturer, principal investigator, supervisor, and laboratory animal veterinarian. The MicroCAT II control computer will run in a Windows XP environment connected to a high-speed real-time reconstruction engine, and linked to the local networks and the Ohio Supercomputer Center (Columbus, OH), allowing rapid data processing and access from distant sites. Salary and benefits for the Supervisor and Space and utilities for the instrument will be provided by Department of Veterinary Biosciences and the College of Veterinary Medicine.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本次共享仪器拨款请求使用由 CTI Molecular Imaging, Inc.（田纳西州诺克斯维尔）制造的高分辨率微计算机断层扫描系统 MicroCAT II。 MicroCAT 成像系统由能源部橡树岭国家实验室开发，旨在加速基因工程小鼠的筛选。该系统由焦点尺寸 <10 µm 的微焦点 X 射线源和用于高速研究的较大焦点组成。对于 X 射线检测，MicroCAT II 配备高分辨率 16 兆像素 CCD 摄像头，具有用于筛选的大视场 (FOV) 和用于高分辨率成像的小视场 (FOV)。该系统采用铅屏蔽且符合 FDA X 射线标准。所要求的 MicroCAT II 系统的规格将允许 NIH 资助的研究人员对小型实验动物和组织标本进行成像，以满足研究人员的成像需求。与 Amira 软件捆绑在一起的 MicroCAT II 系统将允许非侵入性捕获数据、生成形态信息、使用基于地标的图像配准应用程序进行纵向研究以及疾病建模所需的高分辨率 3D 重建。 俄亥俄州立大学没有 Micro-CT 成像系统。我们有其他方法对实验动物和组织标本进行成像，但是，这些方式对结构分析不太敏感，耗时，常规使用成本昂贵，具有不同的应用，并且不允许纵向研究。 microCT 系统将最大限度地生成高质量信息，并使可用资源的使用更加高效和富有成效。该系统还将补充现有的小动物成像技术。 MicroCAT II系统将允许参与该计划的NIH资助的研究人员完成和改进他们在骨生物学、细胞周期失调和发育、成骨细胞和破骨细胞功能的转录调节、前列腺/乳腺癌和骨转移、雄激素和骨量、生长因子和骨发育、内分泌肿瘤和颅骨发育、骨组织工程、基因传递等方面的研究。 MicroCAT II 系统对于体内纵向研究非常重要，可以避免早期牺牲有价值的研究动物。它对其他研究人员也很有用，因为它可用于对生物材料和软组织间相进行成像。该系统将提高检测差异的速度和能力，从而提高统计能力，使评估大量动物成为可能，并允许体内重复测量。该仪器将位于兽医学院易于进入的生物安全动物室（Sisson Hall），并由研究科学家主管（专门从事成像）和实验动物兽医监督。已经制定了 MicroCAT II 成像系统的放置、维护和操作的组织和管理计划。按小时收取的费用将为主管提供服务合同和继续教育的资金。计费将由兽医生物科学部的影像服务部门进行。主要研究人员已在橡树岭国家实验室和 ImTek, Inc.（田纳西州诺克斯维尔）接受过有关使用 MicroCAT II 系统的培训。用户将接受系统制造商、主要研究者、主管和实验动物兽医的培训。 MicroCAT II 控制计算机将在连接到高速实时重建引擎的 Windows XP 环境中运行，并连接到本地网络和俄亥俄州超级计算机中心（俄亥俄州哥伦布），允许快速数据处理和从远程站点访问。主管的工资和福利以及仪器的空间和公用设施将由兽医生物科学系和兽医学院提供。