SMALL LAB ANIMAL IMAGING: LEUKEMIA, LUNG CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：此共享仪器拨款申请高分辨率微计算机断层扫描系统，MicroCAT II，制造的CTI分子成像公司(诺克斯维尔，田纳西州)。MicroCAT成像系统是由能源部橡树岭国家实验室开发的，目的是加快基因工程小鼠的筛选。该系统由一个焦斑尺寸为10？m的微焦点X射线源和更大的用于高速研究的焦点组成。在X射线探测方面，MicroCAT II拥有1600万像素的高分辨率CCD摄像机，用于筛选的大视场(FOV)和用于高分辨率成像的小视场。该系统是铅屏蔽的，符合FDA的X射线标准。所要求的MicroCAT II系统中的规格将允许NIH资助的研究人员对小型实验室动物和组织样本进行成像，以满足研究人员的成像需求。与Amira软件捆绑在一起的MicroCAT II系统将允许非侵入性地捕获数据、生成形态信息、使用基于里程碑的图像配准应用程序进行纵向研究以及疾病建模所需的高分辨率3D重建。俄亥俄州立大学没有Micro-CT成像系统。我们有替代的方法来对实验室动物和组织标本进行成像，然而，这些方法对结构分析不那么敏感，耗时长，常规使用成本高，有不同的应用，并且不允许进行纵向研究。微CT系统将最大限度地产生高质量的信息，并将使现有资源的使用更有效率和生产力。该系统还将补充现有的小动物成像技术。MicroCAT II系统将允许参与这一倡议的NIH资助的研究人员完成和改进他们在骨生物学、细胞周期失调和发育、成骨细胞和破骨细胞功能的转录调控、前列腺癌/乳腺癌和骨转移、雄激素与骨量、生长因子与骨发育、内分泌肿瘤与头骨发育、骨组织工程、基因传递等方面的研究。MicroCAT II系统对于体内纵向研究将是重要的，避免了有价值的研究动物的早期牺牲。它也将对其他研究人员有用，因为它可以用于对生物材料和软组织中间相进行成像。该系统将提高检测差异的速度和能力，从而增加统计能力，使更多动物的评估成为可能，并允许体内重复测量。该仪器将放置在兽医学院一个容易接近的生物切割室(Sisson Hall)，由一名研究科学家主管(专门从事成像)和一名实验室动物兽医监督。已经为MicroCAT II成像系统的放置、维护和操作制定了一项组织和行政计划。每小时的费用将为主管提供服务合同和继续教育的资金。账单将由兽医生物科学系成像服务执行。首席研究员在橡树岭国家实验室和IMTEK公司(田纳西州诺克斯维尔)接受了有关使用MicroCAT II系统的培训。用户将接受系统制造商、首席调查员、监督员和实验动物兽医的培训。MicroCAT II控制计算机将在Windows XP环境中运行，连接到高速实时重建引擎，并连接到本地网络和俄亥俄州超级计算机中心(俄亥俄州哥伦布市)，允许快速数据处理和从远程站点访问。主管的工资和福利以及仪器的空间和公用事业将由兽医生物科学系和兽医学院提供。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A High Resolution Microcomputed Tomography System, MicroCAT II, manufactured CTI Molecular Imaging, Inc. (Knoxville, TN) is being requested in this Shared Instrumentation Grant. The MicroCAT Imaging System was developed by the Department of Energy's Oak Ridge National Laboratories with the objective of accelerating the screening of genetically- engineered mice. The system consists of a microfocus X-ray source with a <10¿m focal spot size and larger focal spots for high-speed studies. For X-ray detection, the MicroCAT II has a high resolution 16 megapixel CCD camera with a large field of view (FOV) for screening and small FOV for high resolution imaging. The system is lead-shielded and FDA X-ray compliant. Specifications in the requested MicroCAT II system will allow NIH-funded researchers to image small laboratory animals and tissue specimens, fitting the investigators imaging needs. The MicroCAT II system bundled with the Amira Software will allow the non-invasive capture of data, generation of morphological information, longitudinal studies using landmark-based image registration applications, and high resolution 3D reconstructions necessary for disease modeling. There are no Micro-CT imaging systems at the Ohio State University. We have alternate methods to image laboratory animals and tissue specimens, however, these modalities are less sensitive for structural analysis, are time consuming, expensive to be used on a routine basis, have different applications, and do not allow longitudinal studies. A microCT system will maximize the generation of high quality information and will make the use of available resources more efficient and productive. This system will also complement the existing small animal imaging technologies. The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. The MicroCAT II system will be important for in vivo longitudinal studies, avoiding the early sacrifice of valuable research animals. It will also be useful to other researchers because it can be used to image biomaterials and soft tissue interphases. This system will increase the speed and ability to detect differences not possible before, thus increasing statistical power, making possible the evaluation of a larger number of animals, and allowing in vivo repeated measurements. The instrument will be located in a readily accessible biosecured animal room (Sisson Hall) of the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (specialized in imaging) and a Laboratory Animal Veterinarian. An Organizational and Administrative Plan for the placement, maintenance, and operation of the MicroCAT II Imaging System has been formulated. An hourly fee will provide funds for a service contract and continuing education for the supervisor. Billing will be performed by the Imaging Service of the Department of Veterinary Biosciences. The principal investigator has received trained on the use of the MicroCAT II system at Oak Ridge National Labs and at ImTek, Inc. (Knoxville, TN). Users will receive training by the system manufacturer, principal investigator, supervisor, and laboratory animal veterinarian. The MicroCAT II control computer will run in a Windows XP environment connected to a high-speed real-time reconstruction engine, and linked to the local networks and the Ohio Supercomputer Center (Columbus, OH), allowing rapid data processing and access from distant sites. Salary and benefits for the Supervisor and Space and utilities for the instrument will be provided by Department of Veterinary Biosciences and the College of Veterinary Medicine.