A Study of Metal Sequestering and Release from Microbes

微生物中金属的螯合和释放研究

• 批准号: 7274347
• 负责人: THOMAS E JENSEN
• 金额: $ 12.5万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7274347
• 关键词: alginates; atomic absorption spectrometry; bacteria; bacterial toxicology; cell components; cell morphology; cell wall; cytoplasm; environmental exposure; heavy metals; mathematical model; microorganism metabolism; phosphorus; polyphosphates; scanning electron microscopy; transmission electron microscopy

We propose research which will test the hypothesis where we propose that microbial polyphosphate bodies (PPBs) are the main sites of metal sequestering. We will use our technology of a transmission electron microscope (TEM) with a scanning transmission mode (STGM) and an associated energy dispersive spectrometer (EDX) to determine which cell component sequesters the most metal. Spectra obtained with the spot mode setting of the STEM from different cell components will be obtained. The cell components to be analyzed are capsule (sheath) cell wall, cytoplasm and PPBs. We will calculate the volume of the cell each of these components has using the appropriate mathematical formula for the shape. The amount of the metal in each cell area will be determined quantitatively using the Princeton Gamma Tech IMIX-EDX. From this data we will calculate the amount of metal sequestered in each cell area or component. We propose using for test organisms: (1) Plectonema boryanum, (2) Synechococcus leopoliensis, (3) Gloeocapsa alpicola, (4) Pseudomonas aeruginosa, (5) Escherichia coli, (6) Staphlococcus areus, (7) Bacillus subtilis, (8) Acinetobacter calcoaceticus, (9) Arthrobacter globiformis, (10) Saccharomyces cervisae, and (11) Rhodotorula rubra. If our hypothesis is sustained we will develop a metal removing system where cells with large numbers of PPBs are enrobed in Na-alginate. The aerobic system will be converted to an anaerobic environment. The PPBs should be dismantled with the metal being released into the supemant. This should allow the metal to be concentrated many times. The metals and the phosphorus can then be precipated.

我们提出的研究将检验我们提出的假设，即微生物多磷酸体（PPB）是金属螯合的主要位点。我们将使用具有扫描透射模式 (STGM) 的透射电子显微镜 (TEM) 和相关的能量色散光谱仪 (EDX) 技术来确定哪个细胞 成分螯合了最多的金属。将获得通过 STEM 点模式设置从不同细胞成分获得的光谱。待分析的细胞成分是荚膜（鞘）细胞壁、细胞质和 PPB。我们将使用适当的形状数学公式来计算每个组件所具有的单元体积。金额 每个单元区域中的金属将使用 Princeton Gamma Tech IMIX-EDX 进行定量测定。根据这些数据，我们将计算每个单元区域或组件中螯合的金属量。我们建议使用以下测试微生物：(1) Plectonema boryanum、(2) Synechococcus leopoliensis、(3) Gloeocapsa alpicola、(4) Pseudomonas aeruginosa、(5) 大肠杆菌、(6) 葡萄球菌、(7) 枯草芽孢杆菌、(8) 乙酸钙不动杆菌、(9) 球状节杆菌、(10) 酿酒酵母和(11) 红色红酵母。如果我们的假设成立，我们将开发一种金属去除系统，其中含有大量 PPB 的细胞被海藻酸钠包裹。有氧系统将转变为无氧环境。应将 PPB 拆除并释放金属 进入上清液。这应该可以使金属多次浓缩。然后可以沉淀金属和磷。