Identification of protein factors and pathways leading t

鉴定导致 t 的蛋白质因子和途径

• 批准号: 7291718
• 负责人: MIKHAIL KASHLEV
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7291718
• 关键词: Identification; protein; factors; pathways; leading

Epigenetic changes in transcribed regions of the genes, such as DNA methylation, covalent modifications of histones, and ATP-dependent chromatin remodeling, have been recently identified as key universal components that regulate gene expression. Among these epigenetic modificators are large protein ensembles RSC, Swi/Snf, iSwi and histone modification factors that increase accessibility of chromatin to transcription. RNA polymerase itself and the associated transcription elongation factors may also work to establish and maintain the active state of the chromatin.The major results: 1. The yeast chromatin-remodeling complex RSC reduces nucleosomal barrier to Pol II in vitro. The mechanism of the reduction is currently being investigated.2. The yeast transcript cleavage factor TFIIS facilitates transcription through nucleosomes by Pol II. RSC and TFIIS cooperate in the process.3. We determine the fate of nucleosomes after factor-stimulated transcription by Pol II.

基因转录区域的表观遗传学变化，如DNA甲基化、组蛋白共价修饰和ATP依赖的染色质重塑，最近已被确定为调节基因表达的关键通用组分。在这些表观遗传修饰剂中，有大蛋白质集合RSC、Swi/Snf、iSwi和组蛋白修饰因子，它们增加染色质对转录的可及性。RNA聚合酶本身及其相关的转录延伸因子也可能参与染色质活性状态的建立和维持。酵母染色质重塑复合物RSC在体外降低核小体对Pol II的屏障。目前正在研究减少的机制。酵母转录物切割因子TFIIS促进Pol II通过核小体的转录。区域服务中心和森林调查处在这一过程中进行合作。我们确定的命运核小体后，因子刺激转录Pol II。