Monitoring of Basic Biochemical Processes at Single Molecule Level Using Light-e

大分子酶促过程的分析主要基于间接观察。我们开发了一种直接成像方法，其中一个或多个大分子成分被量子点（Q-dots）标记，允许人们实时跟踪单个分子群体的行为。q点纳米生物化学提供了一个前所未有的简单和直接的分析大分子复合物的作用。该系统可用于蛋白质组学和药物发现。我们选择在不干扰DNA、RNA和蛋白质生物活性的情况下，将q点附着在DNA、RNA和蛋白质上，从而率先监测单个大分子事件。该方法提供了体内转录复合物复杂相互作用分析（ChIP分析）与传统批量生物化学结果之间缺失的联系。该系统也可用于蛋白质组学和药物发现。

Analysis of macromolecular enzymatic processes is based largely on indirect observations. We developed a direct imaging approach in which one or more macromolecular components are labeled with Quantum Dots (Q-dots), allowing one to follow the behavior of a population of individual molecules in real-time. Q-dots nano-biochemistry provides an unprecedented simple and direct analysis of macromolecular complexes in action. This system can be adapted for proteomics and drug discovery. We have chosen to pioneer the monitoring of single macromolecular events by the attachment of Q-dots to DNA, RNA and proteins without interfering with their biological activity. This method provides a missing link between analysis of complex interactions in transcription complexes in vivo (ChIP analysis) and results obtained by traditional bulk biochemistry. This system can also be adapted for proteomics and drug discovery.