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Transcription Through Nucleosomes in Vitro by E. coli RN

Transcription Through Nucleosomes in Vitro by E. coli RN
大肠杆菌 RN 通过核小体进行体外转录
批准号：
6951653
负责人：
MIKHAIL KASHLEV
金额：
$0.0万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/6951653
关键词：
Escherichia coli RNA directed DNA polymerase Saccharomyces cerevisiae bacterial genetics biotechnology enzyme activity eukaryote fungal genetics genetic techniques genetic transcription molecular biology nucleosomes polymerase chain reaction protein structure technology /technique development

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英文摘要
This project addresses the intriguing question of how eukaryotic RNA polymerases transcribe through chromatin. The project involves development of the minimal in vitro system for transcription through nucleosome by Pol II, and Pol III from S. cerevisiae. RNAP from E. coli, which behaves very similarly to the eukaryotic enzyme in nucleosomal transcription, is used in this project as an easy-handled prototype of Pol II (Kireeva et al., Molecular Cell, 2002).
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Transcription Through Nucleosomes by RNA Polymerase II
TRANSCRIPTION ELONGATION BY RNA POLYMERASE II
Mechanisms of transcription fidelity in prokaryotes and eukaryotes
  • 批准号：
    9153672
  • 项目类别：
  • 资助金额：
    $74.31万
  • 财政年份：
    --
  • 负责人：
    MIKHAIL KASHLEV
  • 依托单位：
    DIVISION OF BASIC SCIENCES - NCI
Basic Mechanism of Transcription Elongation by E. coli R
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