Transcription Through Nucleosomes in Vitro by E. coli RN

大肠杆菌 RN 通过核小体进行体外转录

• 批准号: 6951653
• 负责人: MIKHAIL KASHLEV
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6951653
• 关键词: Escherichia coli; RNA directed DNA polymerase; Saccharomyces cerevisiae; bacterial genetics; biotechnology; enzyme activity; eukaryote; fungal genetics; genetic techniques; genetic transcription; molecular biology; nucleosomes; polymerase chain reaction; protein structure; technology /technique development

This project addresses the intriguing question of how eukaryotic RNA polymerases transcribe through chromatin. The project involves development of the minimal in vitro system for transcription through nucleosome by Pol II, and Pol III from S. cerevisiae. RNAP from E. coli, which behaves very similarly to the eukaryotic enzyme in nucleosomal transcription, is used in this project as an easy-handled prototype of Pol II (Kireeva et al., Molecular Cell, 2002).

这个项目解决了真核生物RNA聚合酶如何通过染色质转录的有趣问题。该项目涉及开发最小的体外系统，用于由S.啤酒。RNAP来自E.在核小体转录中表现得与真核酶非常相似的大肠杆菌在该项目中用作Pol II的易于处理的原型（Kireeva等人，Molecular Cell，2002）。