Retroviral Double Infection and Its Relationship to Reco

该项目的目标是了解HIV-1双重感染及其对重组和病毒进化的影响。被两种或两种以上基因不同病毒感染的细胞可以产生杂合病毒粒子，由此产生重组病毒。因此，双重感染直接影响整体重组率。我们研究了HIV-1通过直接无细胞病毒感染和细胞介导性病毒传播在培养和原代T细胞中双重感染的性质。我们观察到，在这两种传播途径中，HIV-1双重感染的发生频率明显高于随机感染。这是第一项揭示逆转录病毒双重感染本质的研究。我们还剖析了导致非随机HIV-1双重感染的机制，并证明了进入宿主细胞的偏好在非随机双重感染中起着重要作用。我们目前正在建立其他实验系统，以继续我们对逆转录病毒双重感染的研究，包括检查临床样本。

The goals of this project are to understand HIV-1 double infection and its impact on recombination and viral evolution. Cells infected by two or more genetically different viruses can produce heterozygotic virions, from which recombinant viruses are generated. Therefore, double infection directly affects the overall recombination rate. We have studied the nature of HIV-1 double infection in cultured and primary T cells by direct cell-free virus infection and by cell-mediated virus transmission. We have observed that HIV-1 double infection occurs significantly more frequently than it would at random in both transmission pathways. This is the first study that reveals the nature of retroviral double infection. We have also dissected the mechanisms that cause nonrandom HIV-1 double infection, and demonstrated that preference in entry to the host cell played an important role in nonrandom double infection. We are currently establishing other experimental systems to continue our study of retroviral double infection, including examining clinical samples.