Retroviral Double Infection and Recombination

逆转录病毒双重感染和重组

• 批准号: 6952098
• 负责人: WEI-SHAU HU
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6952098
• 关键词: HIV infections; T lymphocyte; biochemical evolution; cell free system; genetic recombination; human immunodeficiency virus 1; tissue /cell culture; virion; virus infection mechanism

The goals of this project are to understand HIV-1 double infection and its impact on recombination and viral evolution. Cells infected by two or more genetically different viruses can produce heterozygotic virions, from which recombinant viruses are generated. Therefore, double infection directly affects the overall recombination rate. We have studied the nature of HIV-1 double infection in cultured and primary T cells by direct cell-free virus infection and by cell-mediated virus transmission. We have observed that HIV-1 double infection occurs significantly more frequently than it would at random in both transmission pathways. This is the first study that reveals the nature of retroviral double infection. We are now dissecting the mechanisms that cause nonrandom HIV-1 double infection.

该项目的目标是了解HIV-1双重感染及其对重组和病毒进化的影响。被两种或更多种遗传上不同的病毒感染的细胞可以产生杂合病毒体，由此产生重组病毒。因此，双重感染直接影响整体重组率。我们已经研究了HIV-1的双重感染的性质，在培养和原代T细胞直接无细胞病毒感染和细胞介导的病毒传播。我们观察到，HIV-1双重感染在两种传播途径中发生的频率明显高于随机感染。这是第一项揭示逆转录病毒双重感染本质的研究。我们现在正在剖析导致非随机HIV-1双重感染的机制。