Retroviral Double Infection and Recombination

该项目的目标是了解HIV-1双重感染及其对重组和病毒进化的影响。被两种或两种以上基因不同的病毒感染的细胞可以产生杂合子病毒粒子，从中产生重组病毒。因此，双重感染直接影响整体重组率。我们通过直接无细胞病毒感染和细胞介导的病毒传播研究了培养T细胞和原代T细胞中HIV-1双重感染的性质。我们已经观察到，HIV-1双重感染在两种传播途径中发生的频率明显高于随机感染。这是第一个揭示逆转录病毒双重感染本质的研究。我们现在正在剖析导致非随机HIV-1双重感染的机制。

The goals of this project are to understand HIV-1 double infection and its impact on recombination and viral evolution. Cells infected by two or more genetically different viruses can produce heterozygotic virions, from which recombinant viruses are generated. Therefore, double infection directly affects the overall recombination rate. We have studied the nature of HIV-1 double infection in cultured and primary T cells by direct cell-free virus infection and by cell-mediated virus transmission. We have observed that HIV-1 double infection occurs significantly more frequently than it would at random in both transmission pathways. This is the first study that reveals the nature of retroviral double infection. We are now dissecting the mechanisms that cause nonrandom HIV-1 double infection.