Genetic Recombination in Retroviruses

逆转录病毒的基因重组

• 批准号: 7291847
• 负责人: WEI-SHAU HU
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7291847
• 关键词: Genetic; Recombination; Retroviruses

The goals of this project are to determine the frequency and mechanisms of HIV recombination, to characterize potential recombination between divergent lentiviruses, and to examine the properties of recombinant viruses. We have measured HIV-1 recombination rates at several distances and demonstrated that recombination is a very powerful tool for reassorting the HIV-1 genome. We are currently examining whether other lentiviruses can also recombines at high frequencies. We have identified a major restriction to intersubtype HIV-1 recombination, and are currently studying other restrictions to HIV recombination and replication. We have extended these studies to examine whether distinct lentiviruses can recombine with one another. In addition to studying the frequency and limitation of recombination, it is also important to understand the consequences of recombination. To address this aspect of recombination, we are currently examining the properties of recombinant viruses, and whether certain recombinants have a selective advantage in a given environment. Because it is thought that zoonotic events generated both HIV-1 and HIV-2, we have also constructed hybrid viruses to study whether recombination can facilitate the adaptation to new hosts.

该项目的目标是确定HIV重组的频率和机制，表征不同慢病毒之间的潜在重组，并检查重组病毒的特性。我们已经测量了HIV-1在几个距离的重组率，并证明重组是一个非常强大的工具，用于重组HIV-1基因组。我们目前正在研究其他慢病毒是否也能以高频率重组。我们已经确定了亚型间HIV-1重组的主要限制，目前正在研究HIV重组和复制的其他限制。我们已经扩展了这些研究，以检查不同的慢病毒是否可以相互重组。除了研究重组的频率和限制之外，了解重组的后果也很重要。为了解决这方面的重组，我们目前正在研究重组病毒的特性，以及某些重组体是否在给定的环境中具有选择性优势。因为人们认为人畜共患病事件产生了HIV-1和HIV-2，我们还构建了杂交病毒，以研究重组是否有助于适应新的宿主。