Development of optical probes based on encoded aptamers for RNA visualization in live cells

开发基于编码适体的光学探针，用于活细胞中 RNA 可视化

• 批准号: 2886771
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2886771
• 关键词: Development; optical; probes; based; encoded

RNA is the carrier of genetic information from DNA to proteins. In addition, non-coding RNAs have been found to assist in many essential regulatory functions, which are still being discovered and studied. To fully understand RNAs' varied functions, there is increasing interest in developing methods to visualize them in live cells. In this project we propose to develop a series of small-molecule probes which in combination with an encoded aptamer will be used to visualize RNA dynamics in live cells. To achieve this, we will build on recent optical probes developed in our group which have unique photophysical properties; in combination with the proposed aptamers, it will yield the first encoded RNA visualization tool that employs fluorescence lifetime to study RNA dynamics in single cells. We will also aim to develop this further to utilize super-resolution microscopy. This multidisciplinary project will involve the development of new small-molecule optical probes, nucleic acid chemical biology, and live cell microscopy.

RNA是从DNA到蛋白质的遗传信息的载体。此外，已发现非编码RNA有助于许多重要的调控功能，这些功能仍在发现和研究中。为了充分了解RNA的各种功能，人们越来越感兴趣的是开发在活细胞中可视化它们的方法。在这个项目中，我们建议开发一系列的小分子探针，结合编码的适体将被用来可视化在活细胞中的RNA动力学。为了实现这一目标，我们将建立在我们小组最近开发的具有独特生物物理特性的光学探针上;结合所提出的适体，它将产生第一个编码RNA可视化工具，该工具采用荧光寿命来研究单细胞中的RNA动力学。我们还将进一步开发这一技术，以利用超分辨率显微镜。这个多学科项目将涉及新型小分子光学探针、核酸化学生物学和活细胞显微镜的开发。