CORE-- MASS SPECTROMETRY FACILITY

核心——质谱设备

• 批准号: 7299501
• 负责人: JULIE Ann LEARY
• 金额: $ 50.61万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7299501
• 关键词: biomedical facility; genetic translation; mass spectrometry; posttranslational modifications; ribosomal proteins; translation factor; virus infection mechanism

The development and implementation of the mass spectrometry core is essential to the research of the listed principal investigators. In particular the core will provide mass spectral analysis using the proposed bottomup and top-down methods employing both Q-TOF technology as well as FTICR high-resolution analysis. Both a top-down (intact protein identification) and a bottom-up (protein digestion) approach will be used to identify proteins of the various ribosomal complexes. Differences in post-translational modifications will be determined for ribosome complexes which have been subjected to infection by various viruses (hepatitis c, dengue, etc). eif3 factors associated with the ribosome will be analyzed and investigated for the presence of phosphorylation. Both the high-resolution capabilities of the exisiting FTICR and the sensitivity and flexibility of the Q-TOF, in combination with well established sample preparation methods such as IMAC, will allow for the unambiguous determination of protein constituents and associated post-translational modifications.

质谱核心的开发和实施对于所列的研究至关重要。 首席调查员特别是核心将提供质谱分析使用拟议的自下而上 以及采用Q-TOF技术以及FTICR高分辨率分析的自上而下的方法。 自上而下（完整蛋白质鉴定）和自下而上（蛋白质消化）的方法将用于 识别各种核糖体复合物的蛋白质。翻译后修饰的差异将是 测定已被各种病毒感染的核糖体复合物（丙型肝炎， 登革热等）。将分析和研究与核糖体相关的eif 3因子， 磷酸化测量FTICR的高分辨率能力以及灵敏度和灵活性 结合成熟的样品制备方法，如IMAC，将允许 蛋白质组分和相关翻译后修饰的明确测定。