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Cardiac KATP Channels in Health and Disease

健康和疾病中的心脏 KATP 通道

基本信息

批准号：
7210738
负责人：
ANDRE TERZIC
金额：
$ 31.47万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7210738
关键词：
ATP phosphohydrolase Address Adrenergic Agents Arrhythmia Behavior Biological Preservation Biology Cardiac Cardiomyopathies Catalysis Cells Clinical Medicine Complex DNA Defect Disease Disease model Electrophysiology (science)Enzymatic Biochemistry Enzymes Exhibits Figs - dietary Funding Genomics Health Heart Heart Diseases Heart failure Homeostasis Human Ion Channel Kir6.2 channel Knock-out Mechanics Membrane Metabolic Molecular Multienzyme Complexes Mus Mutation Myocardial Myocardium Nucleotides Operative Surgical Procedures Organ Organism Pathologic Pathway interactions Patients Phenotype Physiological Prevention Primary idiopathic dilated cardiomyopathy Proteomics Regulation Role Sarcolemma Screening procedure Self-Injurious Behavior Signal Transduction Specimen Stress Technology Testing Translating Translations Whole Organism adrenergic base cohort concept conformational conversion density novel prevent response stressor

项目摘要

DESCRIPTION (provided by applicant): Cardiac ATP-sensitive K+ (KATP) channels, formed by the pore-forming Kir6.2 and regulatory SUR2A subunits, are characterized by nucleotide-dependent regulation that allows the channel complex to adjust membrane excitability in response to changes in the cellular energetic state. However, it is unknown how cardiac KATP channels translate nucleotide signals into pore gating, what is the full impact of channel activity on cardiac homeostasis, and ultimately whether channel defects contribute to heart disease. In the previous funding period of this proposal we identified an ATPase activity intrinsic to the SUR2A subunit, demonstrated that deficient KATP channel function reduces cardiac tolerance to adrenergic challenge, and discovered KATP channel mutations in initial screening of patients with heart failure. Based on these findings, we here put forward the novel concept that cardiac KATP channels operate as a bi-functional channel/enzyme molecular combination serving a vital role under diverse stressors. Aim #1 will define the molecular mechanisms governing the SUR2A catalysis-based nucleotide gating of the Kir6.2 pore. Aim #2 will establish the impact of KATP channels on prevention of maladaptive structural remodeling, and preservation of energetic and electrical stability in the physiologically and pathologically stressed myocardium. Aim #3 will determine the spectrum of cardiac KATP channel mutations in patients with idiopathic dilated cardiomyopathy, and define the consequences of these mutations on the KATP channel/enzyme phenotype, metabolic sensing and cell adaptation to stress. To this end, we will employ murine knockout and disease models, along with genomic specimens from an existing cohort of patients with cardiomyopathy. The complementary technologies of enzymology, electrophysiology, physiological genomics, high-throughput DNA screening and functional proteomic analysis will be applied to study the cardiac KATP channel at the organism, organ, cellular and molecular levels. Thus, this proposal will provide an integrated understanding of cardiac KATP channels in metabolic signal decoding, stress adaptation, and their impact for clinical medicine.

描述（由申请人提供）：心脏atp敏感K+ (KATP)通道，由成孔Kir6.2和调节性SUR2A亚基形成，其特点是核苷酸依赖性调节，允许通道复合物调节膜兴奋性，以响应细胞能量状态的变化。然而，目前尚不清楚心脏KATP通道如何将核苷酸信号转化为孔门控，通道活性对心脏稳态的全面影响是什么，以及最终通道缺陷是否会导致心脏病。在本提案的前期资助期内，我们发现了SUR2A亚基固有的atp酶活性，证明KATP通道功能缺陷降低了心脏对肾上腺素能挑战的耐受性，并在心力衰竭患者的初始筛查中发现了KATP通道突变。基于这些发现，我们在此提出了新的概念，即心脏KATP通道作为双功能通道/酶分子组合在不同应激源下发挥重要作用。目的1将定义控制Kir6.2孔的SUR2A催化核苷酸门控的分子机制。目的2将在生理和病理应激心肌中建立KATP通道对预防不适应结构重构和保持能量和电稳定性的影响。目的3将确定特发性扩张型心肌病患者的心脏KATP通道突变谱，并确定这些突变对KATP通道/酶表型、代谢感知和细胞适应应激的影响。为此，我们将采用小鼠基因敲除和疾病模型，以及来自现有心肌病患者队列的基因组标本。应用酶学、电生理学、生理基因组学、高通量DNA筛选和功能蛋白质组学分析等互补技术，在机体、器官、细胞和分子水平上研究心脏KATP通道。因此，该建议将提供对心脏KATP通道在代谢信号解码，应激适应及其对临床医学的影响的综合理解。

项目成果

期刊论文数量（50）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

K(ATP) channels process nucleotide signals in muscle thermogenic response.

DOI：
10.3109/10409238.2010.513374
发表时间：
2010-12
期刊：
Critical reviews in biochemistry and molecular biology
影响因子：
6.5
作者：
Reyes S;Park S;Terzic A;Alekseev AE
通讯作者：
Alekseev AE

Proteomic profiling of KATP channel-deficient hypertensive heart maps risk for maladaptive cardiomyopathic outcome.

KATP 通道缺陷型高血压心脏的蛋白质组学分析绘制了适应不良心肌病结果的风险。

DOI：
10.1002/pmic.200800718
发表时间：
2009
期刊：
Proteomics
影响因子：
3.4
作者：
Zlatkovic,Jelena;Arrell,DKent;Kane,GarvanC;Miki,Takashi;Seino,Susumu;Terzic,Andre
通讯作者：
Terzic,Andre

Failing energetics in failing hearts.