Determining the molecular mode of action of HPC product preservatives and characterising antimicrobial resistance in problematic bacterial contaminant

确定 HPC 产品防腐剂的分子作用模式并表征有问题的细菌污染物的抗菌药物耐药性

• 批准号: 2887686
• 金额: --
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2887686
• 关键词: Determining; molecular; mode; action; HPC

Each year, millions of tonnes of home and personal care (HPC) products, including cleaning products, cosmetics andtoiletries are manufacturer globally. To keep these products free from microorganisms, industrial manufacturers addantimicrobial preservatives. However, products occasionally become contaminated with microorganisms, the mostcommon of which are antimicrobial resistant bacteria which can also cause human infections. The HPC industry is undergoing considerable change, needing to reduce the use of environmentally toxic preservatives,consumer pressure to use natural and milder but less effective preservatives, and the urgent requirement to reducesingle-use plastic packaging. The changes are conducive to increased HPC product contamination, and a potential rise inantimicrobial resistant (AMR) bacteria causing outbreaks of infection. We have teamed up with Unilever Research & Development (Port Sunlight, UK) to offer a CASE PhD studentship aimedat filling multiple knowledge gaps in relation to the use of preservatives and new multifunctional product ingredients.Past collaborative PhD training between Cardiff University and Unilever has investigated a range of problematicantimicrobial resistant bacteria including Burkholderia, Pseudomonas and Enterobacteriaceae. These species areconsidered priorities in relation to the development of new HPC preservation strategies. The proposed PhD will examine the mechanisms behind preservative mode of action and resistance in the prioritybacterial contaminants. This knowledge will provide industrial manufacturers with solutions to improve HPCpreservation and preventing the development of further antimicrobial resistance.The project will specifically: 1. Use molecular and genomic techniques to understand how preservatives and multifunctional ingredients work tosuppress or kill bacteria, as well as how the industrial contaminant resist these important antimicrobials. 2. Monitor and identify priority contaminants causing manufacturing incidents using molecular methods including PCR,whole genome sequencing and sequencing the entire DNA content of the industrial products(metagenomic analysis). 3. Understand how large plasmids (called megaplasmids) specifically help priority contaminants survive the harshantimicrobial-rich conditions in HPC products

每年，数百万吨的家庭和个人护理（HPC）产品，包括清洁产品，化妆品和洗浴用品是全球制造商。为了使这些产品免受微生物的侵害，工业制造商会添加抗菌防腐剂。然而，产品偶尔会被微生物污染，其中最常见的是也可能导致人类感染的抗菌素耐药菌。HPC行业正在经历巨大的变化，需要减少对环境有毒的防腐剂的使用，消费者要求使用天然和温和但不太有效的防腐剂，以及迫切需要减少一次性塑料包装。这些变化有助于增加HPC产品污染，以及导致感染爆发的抗菌素耐药（AMR）细菌的潜在增加。我们与联合利华研发部（Port Sunlight，UK）合作，提供CASE博士研究生奖学金，旨在填补与防腐剂和新的多功能产品成分的使用相关的多个知识空白。过去卡迪夫大学和联合利华之间的合作博士培训已经调查了一系列有问题的抗生素耐药细菌，包括伯克霍尔德氏菌，假单胞菌和肠杆菌科。这些物种被认为是优先发展的新HPC保护策略。拟议的博士学位将研究防腐剂的作用模式和优先细菌污染物的抗性背后的机制。这些知识将为工业制造商提供解决方案，以改善HPC的保存和防止进一步的抗菌素耐药性的发展。使用分子和基因组技术来了解防腐剂和多功能成分如何抑制或杀死细菌，以及工业污染物如何抵抗这些重要的抗菌剂。2.使用分子方法，包括PCR、全基因组测序和对工业产品的全部DNA含量进行测序（宏基因组分析），监测和识别导致生产事件的优先污染物。3.了解大质粒（称为megaplasma）如何专门帮助优先污染物在HPC产品中富含抗菌剂的恶劣条件下生存下来