ShEEP Request for the purchase of a research- grade Cell Imaging Multi-mode Reader

项目总结 退伍军人管理局北加州医疗保健系统(VANCHCS)为50多万退伍军人提供服务，是 全国最大的集水区，包括一个很大的农村地区。VANCHCS运营 萨克拉门托退伍军人医学中心(Mather)和退伍军人事务部马丁内斯的研究项目 门诊部。我们进行研究以发现知识，培养退伍军人管理局科学家和医疗保健 领导人，并创造创新，促进退伍军人和国家的医疗保健。我们的生物医学 实验室研发服务进行临床前和临床研究，以了解 影响疾病的分子、基因组和生理水平的生命过程 退伍军人，主要强调癌症。我们研究计划的一些主要目标是 发现新的癌症治疗靶点，更好地理解分子机制和 癌症进展的潜在途径，如治疗耐药性。要实现这些目标 目标：我们运用功能基因组学、代谢组学、生物信息学、遗传学和药理学 Key生理病理功能及其作用机制的研究进展 监管机构，以阐明新疗法的作用机制。来帮助我们的研究 计划达到这些目标，这将直接受益于我们的退伍军人，我们正在为一个细胞申请资金 成像多模式阅读器。这个系统将使我们能够极大地扩展我们的 VANCHCS的研究能力。例如，我们的研究计划将能够执行 在2D和3D细胞培养上的Brightfield和免疫荧光活细胞成像，以及 多种生化检测，如细胞存活率和毒性、细胞迁移和侵袭、 细胞凋亡和坏死、DNA损伤、吞噬、表型分析和代谢组学分析 举几个例子。这一单件设备将有力地加强我们目前的癌症研究 计划并提高我们申请竞争性赠款资金的能力。目前，我们的癌症 研究小组有几项由VA和NIH资助的研究，这些研究将直接受益于该细胞 成像多模阅读器：1)小分子在抗去势中的作用和机制 前列腺癌；2)新的淋巴瘤治疗的临床机制和免疫学研究； 3)靶向类固醇硫酸酯酶和/或Wnt5A治疗晚期前列腺癌；4)靶向 前列腺癌的肿瘤代谢；5)开发有效和可递送的治疗性多肽和 小分子抑制剂作为一种治疗老年结直肠癌患者的新疗法。最终，我们的 目标是确定与癌症相关的治疗靶点，并开发新的治疗方法以更好地治疗 退伍军人。

PROJECT SUMMARY VA Northern California Health Care System (VANCHCS) serves over 500,000 Veterans and is one of the largest catchments in the country, including a large rural component. VANCHCS operates research projects at both the Sacramento VA Medical Center (Mather) and the VA Martinez Outpatient Clinic. We conduct research to discover knowledge, develop VA scientists and health care leaders, and create innovations that advance health care for Veterans and the nation. Our Biomedical Laboratory Research & Development Service conducts preclinical and clinical research to understand life processes from the molecular, genomic, and physiological levels regarding diseases affecting Veterans, with a major emphasis on cancer. Some key objectives of our research program are to identify new cancer therapeutic targets and better understand the molecular mechanisms and pathways underlying cancer progression, such as therapeutics resistance. To accomplish these goals, we employ functional genomics, metabolomics, bioinformatics, genetics, and pharmacological approaches to determine the physiological and pathological functions and action mechanisms of key regulators to elucidate the mechanisms of action of the new therapeutics. To help our research program reach these goals, which will directly benefit our Veterans, we are requesting funds for a Cell Imaging Multi-mode Reader. This system would allow us to greatly expand the breadth of our research capabilities at VANCHCS. For example, our research program would be able to perform brightfield and immunofluorescence live cell imaging on both 2D and 3D cell cultures, as well as a multitude of biochemical assays, such as cell viability and toxicity, cell migration and invasion, apoptosis and necrosis, DNA damage, phagocytosis, phenotypic assays, and metabolomic assays, to name a few. This single piece of equipment would strongly enhance our current Cancer Research program and increase our ability to apply for competitive grant funding. Currently, our Cancer Research group has several VA- and NIH-funded studies that would benefit directly from this Cell Imaging Multi-mode Reader; 1) function and mechanism of small molecules in castration resistant prostate cancer; 2) periclinal mechanistic and immunologic studies for a novel lymphoma treatment; 3) therapeutic targeting steroid sulfatase and /or Wnt5A for advanced prostate cancer; 4) targeting tumor metabolism in prostate cancer; 5) develop potent and deliverable therapeutic peptides and small molecule inhibitors as a novel therapeutic for Veteran colorectal cancer patients. Ultimately, our goal is to identify cancer relevant therapeutic targets and develop novel therapeutics to better treat Veterans.