Synthesis and Characterization of Patterned Microvascular Networks
图案化微血管网络的合成和表征
基本信息
- 批准号:7229462
- 负责人:
- 金额:$ 35.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-05-01 至 2010-02-28
- 项目状态:已结题
- 来源:
- 关键词:AffectArchitectureBlood VesselsCellularityCharacteristicsChemicalsChemistryClassificationCollagen Type IConditionDermalDevelopmentEndopeptidasesEndothelial CellsEngineeringEnvironmentExhibitsExtracellular MatrixExtravasationFibronectinsFutureGelHistologyHumanHyaluronic AcidIn VitroInflammatoryMechanicsMethodsMicrocirculatory BedMoldsOxidative StressPatternPeptide HydrolasesPerfusionPharmaceutical PreparationsProteinsProteoglycanResearch PersonnelRoleShapesSignal TransductionSkinStimulusStructureTechniquesThickTimeTissue EngineeringTissuesTubeVariantWeekWidthWorkbehavior changecell typeclinical applicationconceptcytokinedecorindesigndesireelastomerichigh throughput screeninglithographymethod developmentneutrophilpressureprogramsresearch studyshear stress
项目摘要
DESCRIPTION (provided by applicant): This 5-year proposal will develop methods to synthesize functional microvascular networks in vitro. Central to this work are: 1) the use of lithography to generate three-dimensional (3D) constructs that have open internal topologies in the shape of human microvascular beds, and 2) maturation of as-synthesized structures by sequential optimization of culture conditions (shear stress, lumenal pressure, oxidative stress, and matrix proteolytic activity). Conditions tailored for maturation of cylindrical microvessels, in which the flow profile is easily determined, will serve as a starting point for maturation of microfabricated channels and networks that have non-circular cross-sections. This work will provide culture conditions that enhance the development of 2 characteristic microvascular functions (barrier function and reactivity to cytokines) and will determine to what extent these functions may co-exist in vitro. Temporal correlation between the emergence of these behaviors and changes in histology will suggest strategies to further enhance maturation. This work will also determine how microvascular networks remodel (i.e., how their cross-sectional shapes and network topologies change over time), and will suggest design principles for 3D networks that preserve their initial topologies in the presence of continuous perfusion. More generally, this work will illustrate the unique capabilities of a lithographic approach to tissue engineering. In the short-term, these networks will serve as surrogate human vascular tissues for high-throughput screening of drugs that may perturb or enhance microvascular function. In the long-term, engineered networks may enhance the perfusion of artificial tissues in vitro, and thereby overcome 1 of the primary obstacles in tissue engineering.
描述(由申请人提供):本项目为期5年,将开发体外合成功能性微血管网络的方法。这项工作的核心是:1)利用光刻技术生成具有人体微血管床形状的开放内部拓扑结构的三维(3D)结构,以及2)通过培养条件(剪切应力、管腔压力、氧化应激和基质蛋白水解活性)的顺序优化来成熟合成结构。为圆柱形微血管成熟而定制的条件,其流动剖面很容易确定,将作为具有非圆形截面的微制造通道和网络成熟的起点。这项工作将提供培养条件,以增强两种特征性微血管功能(屏障功能和对细胞因子的反应性)的发展,并将确定这些功能在体外共存的程度。这些行为的出现与组织学变化之间的时间相关性将为进一步促进成熟提供策略。这项工作还将确定微血管网络如何重塑(即,它们的横截面形状和网络拓扑结构如何随时间变化),并将提出在持续灌注存在下保持其初始拓扑结构的3D网络设计原则。更一般地说,这项工作将说明光刻方法对组织工程的独特能力。在短期内,这些网络将作为人体血管组织的替代品,用于高通量筛选可能干扰或增强微血管功能的药物。从长远来看,工程网络可以增强体外人工组织的灌注,从而克服组织工程的主要障碍之一。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('JOE Y TIEN', 18)}}的其他基金
In Vivo Microsurgical Anastomosis of Prevascularized Tissues
预血管化组织的体内显微外科吻合术
- 批准号:
8748615 - 财政年份:2014
- 资助金额:
$ 35.5万 - 项目类别:
In Vivo Microsurgical Anastomosis of Prevascularized Tissues
预血管化组织的体内显微外科吻合术
- 批准号:
8932002 - 财政年份:2014
- 资助金额:
$ 35.5万 - 项目类别:
Engineering Functional Lymphatic Networks in Vitro
体外工程功能性淋巴网络
- 批准号:
7586324 - 财政年份:2009
- 资助金额:
$ 35.5万 - 项目类别:
Engineering Functional Lymphatic Networks in Vitro
体外工程功能性淋巴网络
- 批准号:
7844942 - 财政年份:2009
- 资助金额:
$ 35.5万 - 项目类别:
Synthesis and Characterization of Patterned Microvascular Networks
图案化微血管网络的合成和表征
- 批准号:
7570009 - 财政年份:2006
- 资助金额:
$ 35.5万 - 项目类别:
Synthesis and Characterization of Patterned Microvascular Networks
图案化微血管网络的合成和表征
- 批准号:
7384512 - 财政年份:2006
- 资助金额:
$ 35.5万 - 项目类别:
Synthesis and Characterization of Patterned Microvascular Networks
图案化微血管网络的合成和表征
- 批准号:
7026565 - 财政年份:2006
- 资助金额:
$ 35.5万 - 项目类别:
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