In Vivo Microsurgical Anastomosis of Prevascularized Tissues

预血管化组织的体内显微外科吻合术

• 批准号: 8748615
• 负责人: JOE Y TIEN
• 金额: $ 8.19万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-23 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8748615
• 关键词: Adipocytes; Adipose tissue; Advocate; Anastomosis - action; Blood Circulation; Blood Vessels; Blood flow; Caliber; Cells; Collagen; Defect; Engineering; Excision; Femoral vein; Fibrin; Generations; Implant; In Vitro; Life; Link; Maintenance; Metabolic; Microfluidics; Modeling; Pattern; Perfusion; Pilot Projects; Procedures; Rattus; Route; Site; Testing; Time; Tissue Engineering; Tissue Viability; Tissues; Trauma; Tube; Vascularization; Work; analog; angiogenesis; base; clinically relevant; design; feeding; femoral artery; implantation; in vivo; novel strategies; pressure; public health relevance; response; scaffold; soft tissue; tissue reconstruction; tool; tube feeding; tumor; vasculogenesis

DESCRIPTION (provided by applicant): The two dominant approaches to vascularization of engineered tissues-angiogenesis and vasculogenesis-require several days to generate perfusion in implanted constructs. This proposed R03 pilot study seeks to develop a third approach, in which microsurgical anastomosis of a prevascularized microfluidic tissue to host vessels provides an immediate, physical route to perfusion. This work will implant endothelialized microfluidic collagen- and fibrin-based tissues using the rat femoral artery and vein as recipients, and will analyze the maintenance of perfusion and tissue viability in the construct. The constructs will be densely seeded with adipocytes to model soft tissue replacements. This proof-of-principle study is an important requisite step before such a physical strategy for vascularization can realize its potential as an alternative to angiogenesis and vasculogenesis. This work will provide a broad enabling tool for generating vascularized tissues and a concrete path to engineering vascularized adipose tissue for treatment of large soft tissue defects, as is often seen after tumor resection or major trauma.

描述(申请人提供)：工程化组织血管化的两种主要方法--血管生成和血管生成--需要几天时间才能在植入的结构中产生灌流。这项建议的R03先导研究试图开发第三种方法，在这种方法中，显微外科手术将预先血运丰富的微流体组织与宿主血管吻合，提供了一种直接的、物理的灌流途径。这项工作将植入内皮化的微流控胶原和纤维蛋白为基础的组织，使用大鼠股动脉和静脉作为受体，并将分析在构建中的灌注量和组织活性的维持。这些构建物将密集种植脂肪细胞，以模拟软组织替代。在这种血管形成的物理策略能够实现其作为血管生成和血管生成的替代方案的潜力之前，这一原则验证研究是重要的必要步骤。这项工作将为生成血管化组织提供广泛的使能工具，并为修复肿瘤切除或重大创伤后常见的大型软组织缺损提供一条工程血管脂肪组织的具体途径。