Self-Assembling Peptides for Tissue Engineering

用于组织工程的自组装肽

• 批准号: 7255708
• 负责人: ALAN J. GRODZINSKY
• 金额: $ 128.51万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7255708
• 关键词: 3-Dimensional; Anabolism; Animal Model; Arts; Biological; Biological Sciences; Biomechanics; Biomedical Engineering; Biophysics; Cartilage; Cell Adhesion; Cells; Cellular biology; Chemical Engineering; Chemistry; Chondrocytes; Clinical Sciences; Computer Simulation; Computing Methodologies; Condition; Defect; Electrical Engineering; Endothelial Cells; Engineering; Environment; Fetal Development; Gel; Goals; Grant; Growth Factor; Growth Factor Receptors; Hepatocyte; Image; In Vitro; Individual; Infarction; Injectable; Institution; Integrins; Laboratories; Lead; Learning; Ligands; Liver; Measurement; Mechanics; Modification; Molecular Biology; Molecular and Cellular Biology; Muscle Cells; Myocardium; Nature; Peptides; Physiology; Preparation; Process; Property; Recreation; Research Personnel; Role; Specialist; Structure; Technology; Testing; Time; Tissue Engineering; Tissues; Vascularization; base; cell type; design; human tissue; improved; in vivo; member; programs; protein aminoacid sequence; research study; scaffold

DESCRIPTION (provided by applicant): A fundamental challenge in tissue engineering is the nature and design of an appropriate 3D scaffold. We propose to use self-assembling peptides to engineer the 3D environment of cells with biologic functionality that can be modified and controlled, based on the basic biophysics of the material, which can be tailored for specific cell types. We employ a Partnership that brings together investigators in biophysics, bioengineering, cell biology, molecular biology, physiology, chemistry, and imaging. Team members are specialists in Electrical Engineering (Grodzinsky, PI), Mechanical Engineering (Kamm), Chemical Engineering (Griffith), Biological Sciences (Zhang, Semino, Lee-BWH), Chemistry (Klibanov) and Clinical Science (Lee, Frisbie-CSU). The use of self-assembling peptides in tissue engineering potentially enables the control of cellular adhesion, biomechanical properties, growth factor presentation and/or release, and vascularization. A fundamental theme of this Partnership is that no single tissue engineering approach is suitable for the diverse structure of all tissues. However, our central hypothesis is that by providing a physiologically appropriate, molecularly specific environment that can be modified by design, we can utilize the "core technologies" of the Partnership to improve the approach for a given tissue. This Partnership brings together expertise in several specific tissues, allowing us to interact in ways that traditional individual grants and programs do not provide. Our Specific Aims are (1) Design, & functionalization of peptide sequence of self-assembling peptides for 3D tissue engineering; (2) To explore the basic biophysics of the self-assembling peptide environment using state-of-the-art computational modeling and biophysical measurements; and (3) To explore the role of the self-assembling peptide environment in three major target tissues: myocardium, cartilage, and liver. The lead institution of the Partnership is MIT, with partners from BWH and CSU.

描述(由申请人提供)：组织工程中的一个基本挑战是合适的3D支架的性质和设计。我们建议使用自组装肽来设计细胞的3D环境，具有可以修改和控制的生物功能，基于材料的基本生物物理，可以为特定的细胞类型量身定做。我们雇佣了一个合作伙伴，将生物物理、生物工程、细胞生物学、分子生物学、生理学、化学和成像领域的研究人员聚集在一起。团队成员包括电气工程(Grodzinsky，Pi)、机械工程(KAMM)、化学工程(Griffith)、生物科学(Zhang，Semino，Lee-BWH)、化学(Klibanov)和临床科学(Lee，Frisbie-CSU)的专家。自组装肽在组织工程中的应用可能使控制细胞黏附、生物力学性质、生长因子递送和/或释放以及血管形成成为可能。这一伙伴关系的一个基本主题是，没有一种单一的组织工程方法适合所有组织的不同结构。然而，我们的中心假设是，通过提供一个可以通过设计修改的生理上合适的、分子上特定的环境，我们可以利用伙伴关系的“核心技术”来改进针对特定组织的方法。这一伙伴关系汇集了几个特定组织的专业知识，使我们能够以传统的个人赠款和计划无法提供的方式进行互动。我们的具体目标是：(1)用于3D组织工程的自组装肽的肽序列的设计和功能化；(2)使用最先进的计算模型和生物物理测量来探索自组装肽环境的基本生物物理；以及(3)探索自组装肽环境在三个主要靶组织中的作用：心肌、软骨和肝脏。该伙伴关系的牵头机构是麻省理工学院，合作伙伴来自BWH和CSU。