Immune Modulation by Bacterial Autolysins

描述（由申请方提供）：SecA2辅助蛋白分泌系统是许多革兰氏阳性病原体分泌毒力促进蛋白所必需的，包括炭疽芽孢杆菌（A类）和单核细胞增生李斯特菌（B类）。我们鉴定了两个依赖SecA2的自溶蛋白，它们促进了L.在受感染的动物中的单核细胞增多症，但不影响这种细菌在组织培养细胞中的生长。缺乏p60的工程化细菌突变体的毒力通过表达全长p60而恢复，但不通过表达截短的无催化活性的蛋白质。p60的催化特异性预示其刺激肽聚糖（PGN）以产生或破坏免疫调节PGN片段（胞肽），包括分别为胞壁酰二肽和三肽（MDP和MTP）。MDP和MTP通过作用于Nod家族的胞浆蛋白来影响哺乳动物细胞的细胞因子应答。我们已经发现，p60表达细菌和小分子从这些细菌释放增强巨噬细胞的特异性免疫调节细胞因子的诱导。在这项资助提案中，我们研究了p60如何促进毒力和影响宿主对感染的先天免疫反应。我们的第一个目标是确定PGN消化所需的p60的功能，以及其对细菌毒力和细胞因子基因表达的影响。我们的第二个目的是研究依赖于p60的生物活性的胞肽或小分子的结构和系统发育分布，并测试对该分子的反应是否需要已知的胞肽反应性Nod家族蛋白。对于我们的第三个目标，我们调查的p60的细菌毒力的影响的潜在机制，通过确定如何表达p60和细胞因子诱导的p60影响巨噬细胞对激活刺激的反应。我们的研究将确定这种细菌自溶素有助于临床上重要的细菌病原体的毒力的机制，并开始探索类似的机制是否促进其他革兰氏阳性病原体的毒力，包括生物恐怖主义的潜在代理。 致病菌致病的机制包括破坏宿主免疫反应的策略。这项资助研究了一种可能对许多致命细菌共同的颠覆性策略。我们的研究将确定这种免疫颠覆策略的分子基础，从而可能揭示在细菌感染、疫苗接种和慢性炎症性疾病期间调节炎症的新治疗途径。

DESCRIPTION (provided by applicant): The SecA2 auxiliary protein secretion system is required for secretion of virulence promoting proteins from a number of Gram-positive pathogens, including Bacillus anthracis (category A) and Listeria monocytogenes (category B). We identified two SecA2-dependent autolytic proteins that promote virulence of L. monocytogenes in infected animals, yet do not affect the growth of this bacterium in tissue culture cells. The virulence of engineered bacterial mutants lacking p60 was restored by expression of full-length p60, but not by expression of a truncated, catalytically inactive protein. The catalytic specificity of p60 predicts that it digests peptidoglycan (PGN) to generate or destroy immune modulating PGN fragments (muropeptides), including respectively muramyl di- and tri-peptides (MDP and MTP). MDP and MTP influence mammalian cell cytokine responses by acting on cytosolic proteins of the Nod family. We have found that p60- expressing bacteria and small molecules released from these bacteria enhance the induction of specific immune-regulatory cytokines by macrophages. In this grant proposal we investigate how p60 promotes virulence and affects host innate immune responses to infection. Our first Aim will identify features of p60 that are required for PGN digestion and for its effects on bacterial virulence and cytokine gene expression. Our second Aim investigates the structure and phylogenetic distribution of a p60-dependent biologically active muropeptide or small molecule and tests whether responses to this molecule require known muropeptide-responsive Nod family proteins. For our third Aim, we investigate a potential mechanism for p60's effects on bacterial virulence by determining how expression of p60 and cytokines induced by p60 affect macrophage responses to activating stimuli. Our studies will define the mechanisms by which this bacterial autolysin contributes to the virulence of a clinically important bacterial pathogen and begin to explore whether similar mechanisms promote virulence of other Gram-positive pathogens, including potential agents of bioterrorism. The mechanisms used by pathogenic bacteria to cause disease include strategies to subvert host immune responses. A subversive strategy that may be common to a number of deadly bacteria is studied in this grant. Our studies will define the molecular basis for this strategy of immune subversion and may thus reveal novel therapeutic avenues to modulate inflammation during bacterial infection, vaccination, and chronic inflammatory diseases.