Gene Expression Patterns and Lifestyles in Legionella

军团菌的基因表达模式和生活方式

基本信息

  • 批准号:
    7173850
  • 负责人:
  • 金额:
    $ 58.84万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-02-15 至 2010-01-31
  • 项目状态:
    已结题

项目摘要

Legionella pneumophila, the gram-negative species that causes legionnaires' disease and other acute respiratory diseases, replicates within a wide range of phagocytic host cells. During legionnaires' disease, L. pneumophila replicates in alveolar macrophages. In the environment, it can replicate in an extraordinary range of protozoan hosts. Replication of L. pneumophila in protozoa is thought to occur in both planktonic and biofilm conditions. Biofilms are thought to be a primary source of the organism during outbreaks in both community-acquired and nosocomial infections. Little is known about the molecular basis for the ability of L. pneumophila to survive and multiply within such a wide range of environmental settings. Several investigators have proposed that L. pneumophila undergoes a developmental switch between distinct forms to account for the observed versatility in changing lifestyles. The availability of the complete L. pneumophila genome sequence and a microarray containing all known L. pneumophila genes, open reading frames and intergenic regions will permit the evaluation of several hypotheses that may account for these different physiologic states. In particular we propose to: (1) Test the hypothesis that distinct patterns of gene expression accompany discrete events during intracellular survival and growth of L. pneumophila; RNA from human macrophages will be isolated at various times after infection and the global pattern of Legionella gene expression will be determined using a whole genome Legionella microarray; (2)Test the hypothesis that distinct patterns of gene expression account for the observed phenotypic differences between axenically-grown and protozoan-grown L. pneumophila; RNA will be isolated from Legionella grown in protoza and the pattern of global gene expression will be compared to that of Legionella grown in axenic media as well as to Legionella that have been exposed to environmental stresses; (3) Test the hypothesis that specific patterns of gene expression in the presence of protozoan hosts promote the formation of Legionella-containing biofilms; RNA will be isolated from Legionella containing biofilms and the patterns of gene expression will be compared to the bacteria in the planktonic phase; (4) Develop model networks that describe relevant patterns of gene expression from the different conditions that have been examined to define specific reporters for each Legionella lifestyle or environmental condition.
嗜肺军团菌是引起军团病和其他急性呼吸道疾病的革兰氏阴性菌,可在多种吞噬性宿主细胞内复制。军团病期间,嗜肺乳杆菌在肺泡巨噬细胞中复制。在环境中,它可以在各种各样的原生动物宿主中复制。嗜肺乳杆菌在原生动物中的复制被认为发生在浮游和生物膜条件下。在社区获得性和医院感染暴发期间,生物膜被认为是生物体的主要来源。对于嗜肺乳杆菌在如此广泛的环境条件下存活和繁殖能力的分子基础知之甚少。一些研究人员提出嗜肺乳杆菌经历了不同形式之间的发育转换

项目成果

期刊论文数量(0)
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HOWARD A SHUMAN其他文献

HOWARD A SHUMAN的其他文献

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{{ truncateString('HOWARD A SHUMAN', 18)}}的其他基金

Site-specific Proteolysis of the Legionella Type IV Secretion System
军团菌 IV 型分泌系统的位点特异性蛋白水解
  • 批准号:
    9510237
  • 财政年份:
    2018
  • 资助金额:
    $ 58.84万
  • 项目类别:
Regulation of stress resistance and virulence genes in Acinetobacter baumannii
鲍曼不动杆菌抗逆性和毒力基因的调控
  • 批准号:
    9098590
  • 财政年份:
    2015
  • 资助金额:
    $ 58.84万
  • 项目类别:
Desiccation resistance in Coxiella burnetii
伯内氏立克次体的干燥抗性
  • 批准号:
    8700034
  • 财政年份:
    2014
  • 资助金额:
    $ 58.84万
  • 项目类别:
Host directed chemical genetic screens for antimicrobial activity
宿主定向化学遗传筛选抗菌活性
  • 批准号:
    8448681
  • 财政年份:
    2013
  • 资助金额:
    $ 58.84万
  • 项目类别:
Host directed chemical genetic screens for antimicrobial activity
宿主定向化学遗传筛选抗菌活性
  • 批准号:
    8301303
  • 财政年份:
    2011
  • 资助金额:
    $ 58.84万
  • 项目类别:
Genetics of Monocyte Killing by Bacteria
细菌杀死单核细胞的遗传学
  • 批准号:
    8159644
  • 财政年份:
    2009
  • 资助金额:
    $ 58.84万
  • 项目类别:
Genetics of Monocyte Killing by Bacteria
细菌杀死单核细胞的遗传学
  • 批准号:
    8206789
  • 财政年份:
    2009
  • 资助金额:
    $ 58.84万
  • 项目类别:
Genetics of Monocyte Killing by Bacteria
细菌杀死单核细胞的遗传学
  • 批准号:
    8472434
  • 财政年份:
    2009
  • 资助金额:
    $ 58.84万
  • 项目类别:
Genetics of Monocyte Killing by Bacteria
细菌杀死单核细胞的遗传学
  • 批准号:
    7735942
  • 财政年份:
    2009
  • 资助金额:
    $ 58.84万
  • 项目类别:
Genetics of Monocyte Killing by Bacteria
细菌杀死单核细胞的遗传学
  • 批准号:
    8278661
  • 财政年份:
    2009
  • 资助金额:
    $ 58.84万
  • 项目类别:

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