Next Generation Rapid Diagnostic Tests for Acute Aortic Dissection (AAD)

下一代急性主动脉夹层快速诊断测试 (AAD)

• 批准号: 2889192
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2889192
• 关键词: Next; Generation; Rapid; Diagnostic; Tests

Acute aortic dissection (AAD) is a potentially fatal illness that often affects young individuals, with an annual incidence of 3-4 per 100,000 in the United Kingdom1. Because the predominant symptom of AAD is acute chest and upper back pain, it is difficult to diagnose because it is frequently confused with other cardiovascular disorders such as heart attack2. Without early detection and intervention, the prognosis for AAD patients is bleak, with mortality increasing by 1-2% every hour undiagnosed3. AAD is typically diagnosed with a combination of imaging techniques such as computed tomography (CT), magnetic resonance angiography (MRA), or transoesophageal echocardiography (TOE) 4, but these are not always available in some urgent care facilities. Due to the rapid occurrence of AAD fatalities, an easy-to-use diagnostic test that allows for a faster and more accurate diagnosis in an emergency environment will allow patients to obtain fast, life-saving medical care. Lateral flow tests (LFTs) are membrane-based diagnostic procedures that are easy to use, inexpensive, robust, and widely available. They are most commonly employed for pregnancy testing5, environmental monitoring, and infectious disease monitoring6. However, their usefulness and versatility have only recently become apparent. During the COVID-19 pandemic, LFT technology was quickly developed to identify the virus, leading to global scale testing, allowing monitoring and diagnosis to occur outside of healthcare facilities in community and home settings. Despite their various advantages, LFTs are frequently disregarded as a diagnostic platform due to their lack of sensitivity and selectivity. These issues, however, can be resolved with careful design of the LFTS membrane materials, biological molecules used in the test, and the nanoparticle label.The objective of the project is to create the next generation of LFTs capable of detecting numerous AAD biomarkers at the same time. By investigating membrane materials and nanoparticle labels in LFTs, novel fabrication techniques to produce new membranes with optimum characteristics and applying these optimised LFTS for the detection of specific biomarkers indicative of AAD, it paves the way to tailor material properties and surface chemistry to improve sensitivity and selectivity in LFTs with a range of applications, both commercially available and novel. References:1. Thrumurthy SG, Karthikesalingam A, Patterson BO, Holt PJE, Thompson MM. The diagnosis and management of aortic dissection. BMJ. 2011 Jan 11;344(jan11 1):d8290-d8290. 2. BHF. Aortic Aneurysm, Dissection and Rupture. 2022. 3. Ranasinghe AM, Strong D, Boland B, Bonser RS. Acute aortic dissection. BMJ. 2011 Jul 29;343(jul29 2):d4487-d4487. 4. Olin JW, Fuster V. Acute Aortic Dissection. Arterioscler Thromb Vasc Biol. 2003 Oct;23(10):1721-3. 5. Marino E, Threlfall WR, Schwarze RA. Early conception factor lateral flow assays for pregnancy in the mare. Theriogenology. 2009 Apr;71(6):877-83. 6. Wang C, Wang C, Wang X, Wang K, Zhu Y, Rong Z, et al. Magnetic SERS Strip for Sensitive and Simultaneous Detection of Respiratory Viruses. ACS Appl Mater Interfaces. 2019 May 29;11(21):19495-505.

急性主动脉夹层（AAD）是一种潜在的致命性疾病，通常影响年轻人，在英国的年发病率为每10万人中有3-4人1。由于AAD的主要症状是急性胸痛和上背痛，因此很难诊断，因为它经常与其他心血管疾病（如心脏病发作）混淆。如果没有早期发现和干预，AAD患者的预后是暗淡的，未诊断的死亡率每小时增加1-2%。AAD通常通过计算机断层扫描（CT）、磁共振血管造影（MRA）或经食管超声心动图（TOE）4等成像技术的组合进行诊断，但在一些紧急护理机构中并不总是可用。由于AAD死亡的快速发生，一种易于使用的诊断测试，允许在紧急环境中更快，更准确的诊断，将使患者获得快速，挽救生命的医疗护理。侧流试验（LFT）是一种基于膜的诊断程序，易于使用，价格低廉，可靠且广泛可用。它们最常用于妊娠测试5、环境监测和传染病监测6。然而，其实用性和多功能性直到最近才变得明显。在COVID-19大流行期间，LFT技术迅速发展以识别病毒，导致全球规模的测试，允许在社区和家庭环境中的医疗保健设施之外进行监测和诊断。尽管LFT具有各种优势，但由于其缺乏灵敏度和选择性，因此经常被忽视为诊断平台。然而，这些问题可以通过仔细设计LFTS膜材料、测试中使用的生物分子和纳米颗粒标签来解决。该项目的目标是创建能够同时检测众多AAD生物标志物的下一代LFTS。通过研究LFT中的膜材料和纳米颗粒标记物，生产具有最佳特性的新膜的新型制造技术，并将这些优化的LFTS用于检测指示AAD的特定生物标志物，它为定制材料特性和表面化学铺平了道路，以提高LFT的灵敏度和选择性，具有一系列应用，包括市售的和新型的。参考文献：1. Thrumurthy SG，Karthikesalingam A，Patterson BO，Holt PJE，Thompson MM.主动脉夹层的诊断和治疗。英国医学杂志。2011年1月11日;344（1月11日）：d8290-d8290。2. BHF。主动脉瘤、夹层和破裂。2022. 3. Ranasinghe AM，Strong D，Boland B，Bonser RS.急性主动脉夹层。英国医学杂志。2011年7月29日;343（7月29日2）：d4487-d4487。4. Olin JW，Fuster V.急性主动脉夹层。动脉血栓血管生物学2003年10月;23（10）：1721-3。5. Marino E，Threlfall WR，Schwarze RA.母马妊娠早期受孕因子侧流测定。兽生学2009年4月;71（6）：877-83。6.王春，王春，王霞，王凯，朱毅，荣忠，等.磁性Sers试纸条用于呼吸道病毒的灵敏和同步检测。ACS应用程序主接口。2019年5月29日;11（21）：19495-505。