UCSF High Throughput Microscope
加州大学旧金山分校高通量显微镜
基本信息
- 批准号:7213091
- 负责人:
- 金额:$ 49.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-06-10 至 2008-06-09
- 项目状态:已结题
- 来源:
- 关键词:AffectAntibodiesApplications GrantsArtsBiochemical PathwayCaliforniaCellsCollectionContractsCore FacilityData AnalysesDatabasesDepthDrug effect disorderElementsEnergy TransferEquipmentGrowth FactorHormonesHourImage AnalysisInterventionLibrariesLifeLocationMaintenanceManufacturer NameMeasurementMeasuresMicroscopeMicroscopicMicroscopyMolecularMolecular ConformationNumbersOperative Surgical ProceduresOpticsOrganOrgan Culture TechniquesPathway interactionsPriceProtein ConformationProteinsResearch PersonnelResearch Project GrantsResourcesRoboticsSan FranciscoSignal TransductionStagingStaining methodStainsStem cellsSupport of ResearchSystemTechniquesTimeUnited States National Institutes of HealthUniversitiescellular imagingcostfluorophorehuman diseaseinstrumentknock-downresponse
项目摘要
DESCRIPTION (provided by applicant): This Shared Instrument Grant application is for a state-of-the-art High Throughput Microscope that will facilitate the studies by 15 defined investigators at the University of California San Francisco. Resources from two established Core Facilities will provide substantial support for the operation and maintenance of this Instrument. This plan will permit access to this equipment by an even larger number of NIH-supported research projects at UCSF (833 Research Grants in 2004). The manufacturer has agreed to lower the price of maintenance contracts by 20%. This will reduce operating costs to facilitate high throughput studies of the complexities and cross-talk in biochemical pathways that affect biologic response and drug action. The NIH has identified high throughput studies and capabilities as a key element of its Roadmap. Uncovering, analyzing and understanding biologic complexities depend upon the ability to conduct highly multiplexed studies with large numbers of variables. End-stage markers of signaling and even the direct interactions, conformation and subcellular partitioning of components of the signaling cascade itself, are readily measured using fluorescent microscopic techniques. For example, the applicant investigators routinely use antibody staining to track the amounts and locations of multiple fluorescent-tagged factors in cell, stem cell or organ cultures treated with hormones, growth factors and molecular or pharmacologic agents. For some studies, the amounts and locations of fluorophore-marked proteins or cells are tracked with time in live cell or organ cultures. We also routinely characterize the interactions and conformations of proteins flagged with different fluorescent proteins through measurements of the amounts of energy transferred between the fluorescent proteins. Our studies now demand that we follow the cellular effects of multiple (and even large libraries of) growth factors, hormones, pharmacologic agents and molecular over- expressions/knock-downs. However, conventional microscopy severely constrains the amount of time required to manually collect and analyze images, thereby limiting important studies. The High Throughput Microscopy system selected, the Cellomics ArrayScan VTI with integrated plate handling, robotic compound delivery, data analysis and database managing capabilities, will allow our investigators to accomplish in hours what previously had required months of effort. An incubation chamber will permit live cell imaging, and an Apotome attachment will permit the optional collection of specific optical sections, in three dimensional organ or cell multi-well cultures. In short, the installation of the proposed equipment would dramatically increase the ability of our investigators to make rapid and deep inroads into the understanding of pathways and interventions for a wide variety of human diseases.
描述(由申请人提供):此共享仪器补助金申请用于最先进的高分辨率显微镜,将促进加州大学弗朗西斯科分校15名指定研究人员的研究。来自两个已建立的核心设施的资源将为该文书的运行和维护提供实质性支持。该计划将允许更多的美国国立卫生研究院支持的加州大学旧金山分校的研究项目使用该设备(2004年获得833项研究赠款)。制造商已同意将维修合同的价格降低20%。这将降低运营成本,以促进对影响生物反应和药物作用的生物化学途径中的复杂性和串扰的高通量研究。NIH已将高通量研究和能力确定为其路线图的关键要素。揭示、分析和理解生物学的复杂性取决于对大量变量进行高度多重研究的能力。信号传导的终末期标志物,甚至信号传导级联本身的组分的直接相互作用、构象和亚细胞分配,都很容易使用荧光显微镜技术测量。例如,申请人研究者常规使用抗体染色来追踪用激素、生长因子和分子或药理学试剂处理的细胞、干细胞或器官培养物中多种荧光标记因子的量和位置。对于一些研究,荧光团标记的蛋白质或细胞的数量和位置在活细胞或器官培养物中随时间跟踪。我们还经常通过测量荧光蛋白之间转移的能量来表征用不同荧光蛋白标记的蛋白质的相互作用和构象。我们的研究现在要求我们遵循多种(甚至是大量的)生长因子、激素、药理学试剂和分子过表达/敲低的细胞效应。然而,传统的显微镜严重限制了手动收集和分析图像所需的时间,从而限制了重要的研究。选择的高重复性显微镜系统Cellomics ArrayScan VTI具有集成的平板处理、机器人化合物输送、数据分析和数据库管理功能,将使我们的研究人员能够在数小时内完成以前需要数月的工作。孵育室将允许活细胞成像,并且Apotome附件将允许在三维器官或细胞多孔培养物中可选地收集特定的光学切片。简而言之,拟议设备的安装将大大提高我们的研究人员的能力,使他们能够快速深入地了解各种人类疾病的途径和干预措施。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Fred J SCHAUFELE', 18)}}的其他基金
Insulin/TZD regulation of protein structure in fat cells
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$ 49.97万 - 项目类别:
Insulin/TZD regulation of protein structure in fat cells
胰岛素/TZD 对脂肪细胞蛋白质结构的调节
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6556578 - 财政年份:2003
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$ 49.97万 - 项目类别:
Transcription factor interactions at the GH promoter
GH 启动子处的转录因子相互作用
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7185168 - 财政年份:1999
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$ 49.97万 - 项目类别:
Transcription factor interactions at the GH promoter
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6777165 - 财政年份:1999
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$ 49.97万 - 项目类别:
TRANSCRIPTION FACTOR INTERACTIONS AT THE GH PROMOTER
GH 启动子处的转录因子相互作用
- 批准号:
2843564 - 财政年份:1999
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Transcription factor interactions at the GH promoter
GH 启动子处的转录因子相互作用
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6850683 - 财政年份:1999
- 资助金额:
$ 49.97万 - 项目类别:
TRANSCRIPTION FACTOR INTERACTIONS AT THE GH PROMOTER
GH 启动子处的转录因子相互作用
- 批准号:
6177957 - 财政年份:1999
- 资助金额:
$ 49.97万 - 项目类别:
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GH 启动子处的转录因子相互作用
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6381203 - 财政年份:1999
- 资助金额:
$ 49.97万 - 项目类别:
Transcription factor interactions at the GH promoter
GH 启动子处的转录因子相互作用
- 批准号:
7367018 - 财政年份:1999
- 资助金额:
$ 49.97万 - 项目类别:
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