Core C Microscopy

摘要/概要 核心C：显微镜核心。糖尿病研究依赖于对细胞内部空间关系的研究。 细胞内的组织或结构，以及这些位点上分子因子的相对位置和数量。 显微镜检查能够在疾病开始时和/或在治疗期间检测这些差异的改变。 作为糖尿病研究的一个组成部分而研究的正常发育和生理过程。 目的：显微镜核心整合，增强和传播组织资源和专业知识 和细胞成像技术。核心提供了以下功能，在过去2年中， 由22个刚果民主共和国实验室的122名研究人员完成。在过去四年中，DRC的平均使用时间为2，332小时/年。 1.荧光显微镜三个光学切片（两个共焦;一个Apotome）和两个宽视野 具有高度互补能力的显微镜出现在核心中。多达五种不同的标记物可以 在单个样本中成像。倒置显微镜也被改装用于活细胞成像研究。 2.高分辨率荧光显微镜。机器人每天可以收集多达80，000个字段 用于大规模识别影响微观可测量过程的化合物或因素的系统。 3.组织学。三个切片机，一个低温恒温器，一个组织处理器，一个组织包埋器和一个明场 显微镜可在核心。 4.图像处理。三台计算机专门用于采集后图像分析。 5.信息和培训。核心对调查人员进行设备使用培训，并提供有关 该公司是成像技术的应用，并提供用于显微镜应用的软件和试剂。这 包括建议和/或援助与多光子和电子显微镜可在其他UCSF核心。 对DRC社区的好处：核心通过为DRC研究人员提供访问权限来加速糖尿病研究 以实用和经济的方式应用于先进的成像技术。27名NIH资助的和29名其他糖尿病患者- 这些努力目前使相关项目受益，每年直接费用共计13 769 481美元。 技术开发：核心不断发展，以满足需求。在过去的四年里， 在运行期间，现有设备将继续在堆芯再充机制下得到维护。New Zeiss Apotome和Leica SP 5共聚焦显微镜是通过共享设备赠款购买的， 部门资金和捐助者的礼物。刚果民主共和国调查人员能够使用电子显微镜 在DRC核心经理管理他人拥有的显微镜的安排下。为所有 收购，DRC显微镜核心定义的设备需求，获得的仪器和培训 调查人员使用设备。DRC批准的开发活动项下的其他服务 执行委员会包括对染色的整体组织进行三维成像。这些机制 将继续根据刚果民主共和国的需要发展核心。

Abstract/Summary Core C: Microscopy Core. Diabetes research depends on studies of the spatial relationships of cells within tissues or structures within cells, and the relative positions and amounts of molecular factors at those sites. Microscopy enables the detection of alterations in those differences upon the initiation of disease and/or during the normal developmental and physiologic processes investigated as a component of diabetes research. Purpose: The Microscopy Core consolidates, enhances and disseminates resources and expertise in tissue and cell imaging technologies. The Core provides the following capabilities that, in the past 2 years, were used by 122 researchers in 22 DRC laboratories. DRC use averaged 2,332 hours/year over the last four years. 1. Fluorescence Microscopy. Three optical sectioning (two confocal; one Apotome) and two widefield microscopes with highly complementary capabilities are present in the Core. Up to five different markers may be imaged in a single sample. The inverted microscopes also are retrofitted for live cell imaging studies. 2. High Throughput Fluorescence Microscopy. Up to 80,000 fields per day can be collected on a robotic system for large-scale identification of compounds or factors affecting microscopically measurable processes. 3. Histology. Three microtomes, a cryostat, a tissue processor, a histoembedder and a brightfield microscope are available in the Core. 4. Image Processing. Three computers are available exclusively for post-acquisition image analysis. 5. Information and Training. The Core trains investigators on use of equipment, provides advice on the application of imaging technology, and provides software and reagents for microscopy applications. This includes advice and/or assistance with multiphoton and electron microscopy available in other UCSF Cores. Benefits to DRC Community: The Core accelerates diabetes research by providing DRC investigators access to advanced imaging technology in a practical and economic fashion. 27 NIH-funded and 29 other diabetes- related projects totaling $13,769,481 in annual direct costs currently benefit through these efforts. Technology Development: The Core continues to evolve to meet demand. Over the past four years of operation, existing equipment continues to be maintained under Core recharge mechanisms. New Zeiss Apotome and Leica SP5 confocal microscopes were purchased through shared equipment grants, departmental funds and gifts from donors. Access by DRC investigators to electron microscopy was enabled under an arrangement in which the DRC Core Manager manages microscopes owned by others. For all acquisitions, the DRC Microscopy Core defined equipment needs, acquired the instruments and trained investigators in use of the equipment. Other services under development activities approved by the DRC Executive Committee include three-dimensional imaging on stained, whole-mount tissues. These mechanisms for providing instruments and support will continue to evolve the Core in alignment with DRC needs.