Core C Microscopy

摘要/摘要 芯C：显微镜芯。糖尿病的研究依赖于对细胞内部空间关系的研究 细胞内的组织或结构，以及分子因子在这些部位的相对位置和数量。 显微镜能够在疾病开始时和/或期间检测这些差异的变化 作为糖尿病研究的一部分而调查的正常发育和生理过程。 目的：显微镜核心巩固、加强和传播组织方面的资源和专业知识 和细胞成像技术。核心提供了在过去两年中使用的以下功能 来自22个刚果民主共和国实验室的122名研究人员。在过去四年中，DRC的平均使用时间为2332小时/年。 1.荧光显微镜。三个光学切片(两个共焦；一个Apotome)和两个宽视场 核心中存在着具有高度互补性的显微镜。最多五个不同的标记可以 在单个样本中进行成像。倒置显微镜也被改装用于活细胞成像研究。 2.高通量荧光显微镜。机器人每天最多可采集8万个田地 用于大规模鉴定化合物或影响显微可测量过程的因素的系统。 3.组织学。三个显微切割机，一个低温恒温器，一个组织处理器，一个组织嵌入器和一个Brightfield 核心中有显微镜可用。 4.图像处理。有三台计算机专门用于采集后图像分析。 5.信息和培训。CORE对调查人员进行设备使用方面的培训，提供有关 成像技术的应用，并为显微镜应用提供软件和试剂。这 包括在其他UCSF岩芯中提供的多光子和电子显微镜方面的建议和/或帮助。 对DRC社区的好处：Core通过为DRC调查人员提供访问权限来加速糖尿病研究 以实用和经济的方式发展先进的成像技术。27名NIH资助的糖尿病患者和29名其他糖尿病患者- 相关项目的年度直接费用总额为13 769 481美元，目前受益于这些努力。 技术开发：核心不断发展以满足需求。在过去的四年里 在业务运作期间，现有设备将继续在核心补给机制下进行维护。新蔡司 Apotome和徕卡SP5共焦显微镜是通过共享设备赠款购买的， 各部门的资金和捐助者赠送的礼物。刚果(金)调查人员能够使用电子显微镜 在一种安排下，刚果民主共和国核心经理管理其他人拥有的显微镜。为所有人 采购，刚果民主共和国显微镜核心确定了设备需求，采购了仪器并进行了培训 调查人员正在使用这些设备。刚果民主共和国批准的发展活动中的其他服务 执行委员会包括对染色的整体组织进行三维成像。这些机制 为了提供工具和支持，核心将继续根据刚果民主共和国的需要而发展。

Abstract/Summary Core C: Microscopy Core. Diabetes research depends on studies of the spatial relationships of cells within tissues or structures within cells, and the relative positions and amounts of molecular factors at those sites. Microscopy enables the detection of alterations in those differences upon the initiation of disease and/or during the normal developmental and physiologic processes investigated as a component of diabetes research. Purpose: The Microscopy Core consolidates, enhances and disseminates resources and expertise in tissue and cell imaging technologies. The Core provides the following capabilities that, in the past 2 years, were used by 122 researchers in 22 DRC laboratories. DRC use averaged 2,332 hours/year over the last four years. 1. Fluorescence Microscopy. Three optical sectioning (two confocal; one Apotome) and two widefield microscopes with highly complementary capabilities are present in the Core. Up to five different markers may be imaged in a single sample. The inverted microscopes also are retrofitted for live cell imaging studies. 2. High Throughput Fluorescence Microscopy. Up to 80,000 fields per day can be collected on a robotic system for large-scale identification of compounds or factors affecting microscopically measurable processes. 3. Histology. Three microtomes, a cryostat, a tissue processor, a histoembedder and a brightfield microscope are available in the Core. 4. Image Processing. Three computers are available exclusively for post-acquisition image analysis. 5. Information and Training. The Core trains investigators on use of equipment, provides advice on the application of imaging technology, and provides software and reagents for microscopy applications. This includes advice and/or assistance with multiphoton and electron microscopy available in other UCSF Cores. Benefits to DRC Community: The Core accelerates diabetes research by providing DRC investigators access to advanced imaging technology in a practical and economic fashion. 27 NIH-funded and 29 other diabetes- related projects totaling $13,769,481 in annual direct costs currently benefit through these efforts. Technology Development: The Core continues to evolve to meet demand. Over the past four years of operation, existing equipment continues to be maintained under Core recharge mechanisms. New Zeiss Apotome and Leica SP5 confocal microscopes were purchased through shared equipment grants, departmental funds and gifts from donors. Access by DRC investigators to electron microscopy was enabled under an arrangement in which the DRC Core Manager manages microscopes owned by others. For all acquisitions, the DRC Microscopy Core defined equipment needs, acquired the instruments and trained investigators in use of the equipment. Other services under development activities approved by the DRC Executive Committee include three-dimensional imaging on stained, whole-mount tissues. These mechanisms for providing instruments and support will continue to evolve the Core in alignment with DRC needs.