Core C Microscopy

核心 C 显微镜

• 批准号: 9274292
• 负责人: Fred J SCHAUFELE
• 金额: $ 10.15万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9274292
• 关键词: Adoption; Affect; Brightfield Microscopy; Cells; Communities; Computer software; Computers; Data Collection; Detection; Development; Developmental Process; Diabetes Mellitus; Direct Costs; Disease; Economics; Electron Microscopy; Ensure; Equipment; Fluorescence Microscopy; Funding; Funding Agency; Gifts; Goals; Grant; Histology; Hour; Image; Image Analysis; Imaging Techniques; Imaging technology; Individual; Label; Laboratories; Maintenance; Measurable; Methodology; Methods; Microscope; Microscopic; Microscopy; Microtome - medical device; Molecular; Optics; Organ; Physiological Processes; Positioning Attribute; Process; Proteins; Protocols documentation; Quality Control; Reagent; Research; Research Personnel; Resources; Robotics; Sampling; Services; Site; Staining method; Stains; Structure; System; Techniques; Technology; Three-Dimensional Imaging; Tissue imaging; Tissues; Training; Training Programs; United States National Institutes of Health; Work; cellular imaging; cost; cost effective; cost effectiveness; cryostat; experimental study; image processing; imaging study; innovation; instrument; instrumentation; investigator training; live cell imaging; member; microscopic imaging; operation; programs; spatial relationship; technology development; tool

Abstract/Summary Core C: Microscopy Core. Diabetes research depends on studies of the spatial relationships of cells within tissues or structures within cells, and the relative positions and amounts of molecular factors at those sites. Microscopy enables the detection of alterations in those differences upon the initiation of disease and/or during the normal developmental and physiologic processes investigated as a component of diabetes research. Purpose: The Microscopy Core consolidates, enhances and disseminates resources and expertise in tissue and cell imaging technologies. The Core provides the following capabilities that, in the past 2 years, were used by 122 researchers in 22 DRC laboratories. DRC use averaged 2,332 hours/year over the last four years. 1. Fluorescence Microscopy. Three optical sectioning (two confocal; one Apotome) and two widefield microscopes with highly complementary capabilities are present in the Core. Up to five different markers may be imaged in a single sample. The inverted microscopes also are retrofitted for live cell imaging studies. 2. High Throughput Fluorescence Microscopy. Up to 80,000 fields per day can be collected on a robotic system for large-scale identification of compounds or factors affecting microscopically measurable processes. 3. Histology. Three microtomes, a cryostat, a tissue processor, a histoembedder and a brightfield microscope are available in the Core. 4. Image Processing. Three computers are available exclusively for post-acquisition image analysis. 5. Information and Training. The Core trains investigators on use of equipment, provides advice on the application of imaging technology, and provides software and reagents for microscopy applications. This includes advice and/or assistance with multiphoton and electron microscopy available in other UCSF Cores. Benefits to DRC Community: The Core accelerates diabetes research by providing DRC investigators access to advanced imaging technology in a practical and economic fashion. 27 NIH-funded and 29 other diabetes- related projects totaling $13,769,481 in annual direct costs currently benefit through these efforts. Technology Development: The Core continues to evolve to meet demand. Over the past four years of operation, existing equipment continues to be maintained under Core recharge mechanisms. New Zeiss Apotome and Leica SP5 confocal microscopes were purchased through shared equipment grants, departmental funds and gifts from donors. Access by DRC investigators to electron microscopy was enabled under an arrangement in which the DRC Core Manager manages microscopes owned by others. For all acquisitions, the DRC Microscopy Core defined equipment needs, acquired the instruments and trained investigators in use of the equipment. Other services under development activities approved by the DRC Executive Committee include three-dimensional imaging on stained, whole-mount tissues. These mechanisms for providing instruments and support will continue to evolve the Core in alignment with DRC needs.

摘要/总结 核心 C：显微镜核心。糖尿病研究取决于对体内细胞空间关系的研究 细胞内的组织或结构，以及这些部位分子因子的相对位置和数量。 显微镜能够在疾病开始时和/或在疾病发生期间检测这些差异的变化。 作为糖尿病研究的一部分进行研究的正常发育和生理过程。 目的：显微镜核心整合、增强和传播组织资源和专业知识 和细胞成像技术。该核心提供了过去 2 年中使用的以下功能 由 22 个 DRC 实验室的 122 名研究人员组成。过去四年，DRC 平均使用时间为 2,332 小时/年。 1.荧光显微镜。三个光学切片（两个共焦；一个 Apotome）和两个宽场 核心中存在具有高度互补功能的显微镜。最多可以有五个不同的标记 在单个样本中成像。倒置显微镜还经过改造，可用于活细胞成像研究。 2. 高通量荧光显微镜。机器人每天最多可以收集 80,000 个字段 用于大规模识别影响微观可测量过程的化合物或因素的系统。 3.组织学。三台切片机、一台低温恒温器、一台组织处理机、一台组织包埋机和一台明场 显微镜可在核心中使用。 4.图像处理。三台计算机专门用于采集后图像分析。 5. 信息和培训。核心对调查人员进行设备使用方面的培训，并提供有关 成像技术的应用，并提供显微镜应用的软件和试剂。这 包括其他 UCSF 核心中提供的多光子和电子显微镜方面的建议和/或帮助。 对 DRC 社区的好处：核心通过为 DRC 研究人员提供访问权限来加速糖尿病研究 以实用且经济的方式采用先进的成像技术。 27 项由 NIH 资助的项目和 29 项其他糖尿病项目- 相关项目目前通过这些努力受益，年度直接费用总计 13,769,481 美元。 技术开发：核心不断发展以满足需求。在过去的四年里 运营中，现有设备继续在核心充电机制下进行维护。新蔡司 Apotome 和 Leica SP5 共焦显微镜是通过共享设备赠款购买的， 部门资金和捐助者的礼物。刚果民主共和国研究人员可以使用电子显微镜 根据一项安排，DRC 核心经理管理其他人拥有的显微镜。对于所有人 DRC 显微镜核心定义了设备需求、购买了仪器并接受了培训 调查人员正在使用设备。刚果民主共和国批准的其他正在开发活动的服务 执行委员会包括对染色的整体组织进行三维成像。这些机制 提供工具和支持的公司将继续发展核心以满足刚果民主共和国的需求。