LTQ FT-ICR Hybrid Mass Spectrometer Purchase

描述(由申请人提供)： 这项提议是为了购买一台高性能的LTQ-FTICR质谱仪，该质谱仪将应用于几个与人类健康和疾病有关的项目。为了满足日益增长的对生物分子鉴定、定量和分子表征的准确和快速质量测量的要求，该仪器被要求使用。研究人员已经确定了对改进技术的需求，具体要求是提高灵敏度、高质量准确性、改进量化以及用于蛋白质、肽、脂和低聚糖表征的离子激活的替代方法。所要求的仪器提供了新的和大大改进的能力，并将在广泛的项目中加快国家卫生研究院支持的几名调查人员的生产性研究。这些项目中的大多数都需要该仪器提供的以下几个功能：增强的灵敏度、电子捕获解离、完整蛋白质的“自上而下”质量测量、快速的“MS”扫描速度而不会造成明显的灵敏度损失、能够以依赖数据的方式以高灵敏度快速获取MS3光谱。该仪器将安装在UCDHSC的癌症中心和生物分子结构支持的质谱学/蛋白质组学设施计划中。这种环境确保了科学、技术和管理专业知识的可获得性和参与性，以优化其业绩和研究生产率。霍奇斯博士将担任咨询委员会主席，该委员会负责系统的有效、高效和公平使用。汉森博士将负责仪器的日常操作和维护以及用户培训。由于质谱学/蛋白质组学设施与现在利用这一资源的一群才华横溢、富有成效的研究人员之间的密切联系，生产用途将得到保证。这项收购对UCDHSC的研究人员至关重要，因为目前获得高性能LC-MS“能力的机会非常有限。

DESCRIPTION (provided by applicant): This proposal is for the purchase of a high performance LTQ-FTICR mass spectrometer that will be applied to several projects related to human health and disease. This instrument is required to meet the increasing requirement for the accurate and rapid mass measurements for the identification, quantification and molecular characterization of biomolecules. The investigators have established needs for improved technology with specific requirements for increased sensitivity, high mass accuracy, improved quantification and alternative methods of ion activation for protein, peptide and lipid and oligosaccharides characterization. The requested instrument provides new and greatly improved capabilities and will accelerate the productive research of several NIH supported investigators in a broad range of projects. Most of these projects require several of the following features that are provided by this instrument: enhanced sensitivity, electron capture dissociation, "top down" mass measurements of intact proteins, rapid MS" scan rate without appreciable losses in sensitivity, ability to acquire MS3 spectra rapidly at high sensitivity in a data-dependent manner. This instrument will be housed in the Cancer Center and Program in Biomolecular Structure-supported Mass Spectrometry/Proteomics Facility at UCDHSC. This environment assures the availability and involvement of the scientific, technical and management expertise required to optimize its performance and research productivity. Dr. Hodges will chair the Advisory Committee responsible for productive, efficient and equitable use of the system. Dr. Hansen will be responsible for the day-to-day operation and maintenance of the instrument and user training. Productive use will be assured because of the close association between the Mass Spectrometry/Proteomics Facility and a talented and productive group of researchers that now utilize this resource. This acquisition is critical to researchers at UCDHSC because there is currently very limited access to high performance LC-MS" capabilities.