LTQ FT-ICR Hybrid Mass Spectrometer Purchase

LTQ FT-ICR混合质谱仪采购

• 批准号: 7126187
• 负责人: KIRK C HANSEN
• 金额: $ 106.75万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7126187
• 关键词: 4 year old; Advisory Committees; Amino Acid Sequence; Biological; Calibration; Cancer Center; Cells; Charge; Chromatography; Clinical; Colorado; Complex; Complex Mixtures; Coupled; Cyclotrons; Data; Disease; Dissociation; Electrons; Electrospray Ionization; Ensure; Environment; Fourier Transform; Glutamine; Health; Housing; Human; Hybrids; Inferior; Information Systems; Ions; Light; Lipids; Liquid substance; Maintenance; Marketing; Mass Spectrum Analysis; Measurement; Methods; Modeling; Molecular; Movement; Numbers; Oligonucleotides; Oligosaccharides; Operative Surgical Procedures; Peptide Sequence Determination; Peptides; Performance; Positioning Attribute; Post-Translational Protein Processing; Preparation; Productivity; Proteins; Proteomics; Proteomics Shared Resource; Pulsar; Pump; Range; Rate; Reporting; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Running; Sampling; Scanning; Shapes; Solvents; Source; Speed; Standards of Weights and Measures; Structure; System; Techniques; Technology; Time; Tissue Sample; Training; Tyrosine Phosphorylation; United States National Institutes of Health; Week; Work; Workload; base; data acquisition; day; detector; experience; high throughput analysis; improved; instrument; instrumentation; ionization; mass spectrometer; meter; programs; protein activation; scale up; success; sulfation

DESCRIPTION (provided by applicant): This proposal is for the purchase of a high performance LTQ-FTICR mass spectrometer that will be applied to several projects related to human health and disease. This instrument is required to meet the increasing requirement for the accurate and rapid mass measurements for the identification, quantification and molecular characterization of biomolecules. The investigators have established needs for improved technology with specific requirements for increased sensitivity, high mass accuracy, improved quantification and alternative methods of ion activation for protein, peptide and lipid and oligosaccharides characterization. The requested instrument provides new and greatly improved capabilities and will accelerate the productive research of several NIH supported investigators in a broad range of projects. Most of these projects require several of the following features that are provided by this instrument: enhanced sensitivity, electron capture dissociation, "top down" mass measurements of intact proteins, rapid MS" scan rate without appreciable losses in sensitivity, ability to acquire MS3 spectra rapidly at high sensitivity in a data-dependent manner. This instrument will be housed in the Cancer Center and Program in Biomolecular Structure-supported Mass Spectrometry/Proteomics Facility at UCDHSC. This environment assures the availability and involvement of the scientific, technical and management expertise required to optimize its performance and research productivity. Dr. Hodges will chair the Advisory Committee responsible for productive, efficient and equitable use of the system. Dr. Hansen will be responsible for the day-to-day operation and maintenance of the instrument and user training. Productive use will be assured because of the close association between the Mass Spectrometry/Proteomics Facility and a talented and productive group of researchers that now utilize this resource. This acquisition is critical to researchers at UCDHSC because there is currently very limited access to high performance LC-MS" capabilities.

描述（由申请人提供）： 本提案旨在购买高性能 LTQ-FTICR 质谱仪，该仪器将应用于与人类健康和疾病相关的多个项目。该仪器需要满足对生物分子的识别、定量和分子表征的准确和快速质量测量日益增长的要求。研究人员已经确定了对改进技术的需求，具体要求是提高灵敏度、高质量准确度、改进定量以及蛋白质、肽、脂质和寡糖表征的离子激活替代方法。所需的仪器提供了新的、大大改进的功能，并将加速 NIH 支持的几位研究人员在广泛项目中的富有成效的研究。这些项目中的大多数需要该仪器提供的以下几个功能：增强的灵敏度、电子捕获解离、完整蛋白质的“自上而下”质量测量、快速 MS 扫描速率而不会明显损失灵敏度、以数据依赖的方式以高灵敏度快速获取 MS3 光谱的能力。该仪器将安置在癌症中心和生物分子结构支持的质谱/蛋白质组学项目中 UCDHSC 的设施。这种环境确保了优化其绩效和研究生产力所需的科学、技术和管理专业知识的可用性和参与。霍奇斯博士将担任咨询委员会主席，负责该系统的高效、高效和公平使用。汉森博士将负责仪器的日常操作和维护以及用户培训。由于质谱/蛋白质组学设施和实验室之间的密切联系，将保证生产性使用。 一群才华横溢、富有成效的研究人员现在正在利用这一资源。此次收购对于 UCDHSC 的研究人员至关重要，因为目前获得高性能 LC-MS 功能的机会非常有限。