LTQ FT-ICR Hybrid Mass Spectrometer Purchase

LTQ FT-ICR混合质谱仪采购

• 批准号: 7126187
• 负责人: KIRK C HANSEN
• 金额: $ 106.75万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7126187
• 关键词: 4 year old; Advisory Committees; Amino Acid Sequence; Biological; Calibration; Cancer Center; Cells; Charge; Chromatography; Clinical; Colorado; Complex; Complex Mixtures; Coupled; Cyclotrons; Data; Disease; Dissociation; Electrons; Electrospray Ionization; Ensure; Environment; Fourier Transform; Glutamine; Health; Housing; Human; Hybrids; Inferior; Information Systems; Ions; Light; Lipids; Liquid substance; Maintenance; Marketing; Mass Spectrum Analysis; Measurement; Methods; Modeling; Molecular; Movement; Numbers; Oligonucleotides; Oligosaccharides; Operative Surgical Procedures; Peptide Sequence Determination; Peptides; Performance; Positioning Attribute; Post-Translational Protein Processing; Preparation; Productivity; Proteins; Proteomics; Proteomics Shared Resource; Pulsar; Pump; Range; Rate; Reporting; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Running; Sampling; Scanning; Shapes; Solvents; Source; Speed; Standards of Weights and Measures; Structure; System; Techniques; Technology; Time; Tissue Sample; Training; Tyrosine Phosphorylation; United States National Institutes of Health; Week; Work; Workload; base; data acquisition; day; detector; experience; high throughput analysis; improved; instrument; instrumentation; ionization; mass spectrometer; meter; programs; protein activation; scale up; success; sulfation

DESCRIPTION (provided by applicant): This proposal is for the purchase of a high performance LTQ-FTICR mass spectrometer that will be applied to several projects related to human health and disease. This instrument is required to meet the increasing requirement for the accurate and rapid mass measurements for the identification, quantification and molecular characterization of biomolecules. The investigators have established needs for improved technology with specific requirements for increased sensitivity, high mass accuracy, improved quantification and alternative methods of ion activation for protein, peptide and lipid and oligosaccharides characterization. The requested instrument provides new and greatly improved capabilities and will accelerate the productive research of several NIH supported investigators in a broad range of projects. Most of these projects require several of the following features that are provided by this instrument: enhanced sensitivity, electron capture dissociation, "top down" mass measurements of intact proteins, rapid MS" scan rate without appreciable losses in sensitivity, ability to acquire MS3 spectra rapidly at high sensitivity in a data-dependent manner. This instrument will be housed in the Cancer Center and Program in Biomolecular Structure-supported Mass Spectrometry/Proteomics Facility at UCDHSC. This environment assures the availability and involvement of the scientific, technical and management expertise required to optimize its performance and research productivity. Dr. Hodges will chair the Advisory Committee responsible for productive, efficient and equitable use of the system. Dr. Hansen will be responsible for the day-to-day operation and maintenance of the instrument and user training. Productive use will be assured because of the close association between the Mass Spectrometry/Proteomics Facility and a talented and productive group of researchers that now utilize this resource. This acquisition is critical to researchers at UCDHSC because there is currently very limited access to high performance LC-MS" capabilities.

描述（由申请人提供）： 本建议书旨在采购一台高性能LTQ-FTICR质谱仪，该质谱仪将应用于与人类健康和疾病相关的多个项目。该仪器需要满足日益增长的要求，准确和快速的质量测量的识别，定量和分子表征的生物分子。研究人员已经确定了对改进技术的需求，具体要求是提高灵敏度、高质量准确度、改进定量以及用于蛋白质、肽、脂质和寡糖表征的离子活化替代方法。所要求的仪器提供了新的和大大改进的能力，并将加速几个NIH支持的研究人员在广泛的项目中的生产性研究。这些项目中的大多数都需要该仪器提供的以下几个功能：增强的灵敏度，电子捕获解离，完整蛋白质的“自上而下”质量测量，快速MS扫描速率而不明显损失灵敏度，以数据依赖方式以高灵敏度快速获取MS 3光谱的能力。该仪器将被安置在UCDHSC的癌症中心和生物分子结构支持质谱/蛋白质组学设施项目中。这种环境确保了科学、技术和管理专门知识的可用性和参与，以优化其绩效和研究生产力。Hodges博士将担任咨询委员会主席，负责有效、高效和公平地使用该系统。汉森博士将负责仪器的日常操作和维护以及用户培训。由于质谱/蛋白质组学设施与现在利用这一资源的有才华和富有成效的研究人员小组之间的密切联系，生产性使用将得到保证。此次收购对UCDHSC的研究人员至关重要，因为目前获得高性能LC-MS的能力非常有限。