LTQ Orbitrap Mass Spectrometer Request

描述（由申请人提供）：本申请用于购买混合LTQ-Orbitrap质谱仪，该质谱仪将用于解决与健康和疾病相关的许多生物学问题。该仪器需要满足对生物分子的鉴定、定量和分子表征的准确和快速质量测量的需求和日益增长的需求。研究人员已经确定了对改进技术的需求，具体要求是提高灵敏度、高质量准确度、改善动态范围和提高蛋白质和肽表征的通量。所要求的工具提供了改进的能力，并将加速NIH支持的几个研究人员在广泛项目中的生产性研究。这些项目中的大多数需要该仪器提供的以下几个特征：增强的灵敏度、大的动态范围、高质量精度、以数据依赖的方式在高灵敏度下的快速MSn扫描速率、电子转移解离和鲁棒可靠的操作。该仪器将被安置在UCDHSC的癌症中心和生物分子结构支持质谱/蛋白质组学设施项目中。这种环境确保了科学、技术和管理专门知识的可用性和参与，以优化其绩效和研究生产力。Hodges博士将担任咨询委员会主席，负责仪器使用和访问的总体最大化。汉森博士将负责仪器的日常操作和维护以及培训。通过与质谱/蛋白质组学设施及其监督委员会的密切联系以及机构和仪器用户的财政支持，将确保长期的生产性使用。LTQ-Orbitrap的收购将使大量NIH资助的研究人员能够获得进行重要生物医学发现所需的仪器。该仪器将提供关键的分析能力，从而有利于整个研究界在我们追求改善公共卫生。公共卫生相关性：要求提供资金以购买LTQ-Orbitrap质谱仪。需要这种先进的仪器来做出最终导致生物医学突破和减少与疾病相关的疼痛和痛苦的发现。

DESCRIPTION (provided by applicant): This application is for the purchase of a hybrid LTQ-Orbitrap mass spectrometer that will be used to solve a number of biological questions related to health and disease. This instrument is required to meet the need and growing demand for the accurate and rapid mass measurements for the identification, quantification and molecular characterization of biomolecules. The investigators have established needs for improved technology with specific requirements for increased sensitivity, high mass accuracy, improved dynamic range and higher throughput for protein and peptide characterization. The requested instrument provides improved capabilities and will accelerate the productive research of several NIH supported investigators in a broad range of projects. Most of these projects require several of the following features that are provided by this instrument; enhanced sensitivity, a large dynamic range, high mass accuracy, rapid MSn scan rates at high sensitivity in a data-dependent manner, electron transfer dissociation and robust- reliable operation. This instrument will be housed in the Cancer Center and Program in Biomolecular Structure-supported Mass Spectrometry/Proteomics Facility at UCDHSC. This environment assures the availability and involvement of the scientific, technical and management expertise required to optimize its performance and research productivity. Dr. Hodges will chair the Advisory Committee responsible for overall maximization of instrument usage and access. Dr. Hansen will be responsible for the day-to-day operation and maintenance of the instrument and training. Long term productive usage will be insured by the close association with the Mass Spectrometry/Proteomics Facility and its Oversight Committee and financial support of the institution and instrument users. The acquisition of an LTQ-Orbitrap will allow a large number of NIH funded investigators to have access to the instrumentation required to make important biomedical discoveries. This instrument will provide critical analytical capabilities and thus will be of benefit to the overall research community in our pursuit to improve public health. PUBLIC HEALTH RELEVANCE: Funds are requested to purchase an LTQ-Orbitrap mass spectrometer. This advanced instrument is needed to make discoveries that will ultimately lead to biomedical breakthroughs and a reduction of pain and suffering associated with disease.