Acquisition of a high-throughput high mass accuracy MS for chromatin research
获取用于染色质研究的高通量高质量准确度 MS
基本信息
- 批准号:7215080
- 负责人:
- 金额:$ 50万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-03-06 至 2008-03-05
- 项目状态:已结题
- 来源:
- 关键词:AddressApplications GrantsChemicalsChromatinChromatin StructureCollectionCommunitiesComplementComplexComplex MixturesComputers and Advanced InstrumentationCore FacilityDataElectronsElectrospray IonizationEpigenetic ProcessFundingGrowth and Development functionHealth Services ResearchHistonesHybridsIndustryIonsMalignant NeoplasmsMass Spectrum AnalysisModificationMolecularNatureOhioPatternPost-Translational Protein ProcessingProteomicsRegulationResearchResearch ActivityResearch PersonnelResolutionResourcesRoleSiteSourceSystemTimeUniversitiesVariantcell growthinstrumentinstrumentationinterestionizationmass analyzermass spectrometermedical schoolsnano
项目摘要
DESCRIPTION (provided by applicant): This grant application requests funding to purchase an LTQ Orbitrap mass spectrometer manufactured by Thermo Electron, Finnigan. This instrument is a unique hybrid mass spectrometer that combines a linear ion trap and an orbitrap mass analyzer. The system will come equipped with ESI ionization sources and the surveyor LC system. The LTQ Orbitrap will be located and administered by the Mass Spectrometry and Proteomics (MS&P) core facility in the Campus Chemical Instrument Center at Ohio State University. The orbitrap will fill a critical missing niche in research instrumentation at the Ohio State University. The combined collection of research and service instruments are capable of high-throughput LC-MS/MS (LTQ), molecular formula determination (Q-Tof, FTMS) characterization of complex mixtures that require high resolution (FTMS) and even top-down proteomics (FTMS). However none of the instruments possess the ability to obtain high mass accuracy data for LC-MS and LC-MS/MS. The LTQ Orbitrap nicely complements the currently available instrumentation by adding the ability to perform accurate mass assignments, high mass resolution and multistage MSn at LC time scales. The research activities described in this application address the needs of major users who seek the capabilities of high resolution and high mass accuracy LC-MS and nano-LC-MS/MS to characterize chromatin modifications. The researchers from a core group of users in the Medical School at the Ohio State University. It is understood by all major users that a resource of this importance will not only serve their needs but will contribute greatly to the broader research community at Ohio State. In addition, the CCIC MS facility is designated as the hub facility for the Ohio Mass Spectrometry Consortium. The facility serves the needs of Ohio MS users by making the advanced instrumentation available to the Consortium partners (and to other universities and industries in the Midwest). Thus the instrument will also be available to users in the Ohio Mass Spectrometry Community. Relevance Given the central role of chromatin structure in the proper regulation of cell growth and development, and its potential as a target for epigenetic therapies in cancer, the team of investigators is interested in determining the specific sites and patterns of histone modifications. High-resolution mass spectrometry is crucial to the analysis of histone modifications due to the isobaric nature of histone variants combined with the complex pattern of posttranslational modifications.
描述(由申请人提供):本资助申请要求资助购买由Thermo Electron,Finnigan制造的LTQ Orbitrap质谱仪。该仪器是一种独特的混合质谱仪,结合了线性离子阱和轨道阱质量分析仪。该系统将配备ESI电离源和Surveyor LC系统。LTQ Orbitrap将位于俄亥俄州州立大学校园化学仪器中心的质谱和蛋白质组学(MS&P)核心设施并由其管理。轨道阱将填补俄亥俄州州立大学研究仪器中的一个关键空缺。研究和服务仪器的组合能够进行高通量LC-MS/MS(LTQ)、分子式测定(Q-Tof,FTMS)、需要高分辨率的复杂混合物表征(FTMS),甚至自上而下的蛋白质组学(FTMS)。然而,没有一种仪器能够获得LC-MS和LC-MS/MS的高质量准确度数据。LTQ Orbitrap通过增加在LC时间尺度上执行准确质量分配、高质量分辨率和多级MSn的能力,很好地补充了目前可用的仪器。本申请中描述的研究活动满足了寻求高分辨率和高质量准确度LC-MS和nano-LC-MS/MS表征染色质修饰的主要用户的需求。研究人员来自俄亥俄州州立大学医学院的一个核心用户群体。所有主要用户都明白,这种重要性的资源不仅能满足他们的需求,而且将大大有助于俄亥俄州更广泛的研究界。此外,CCIC MS设施被指定为俄亥俄州质谱联盟的中心设施。该设施通过向联盟合作伙伴(以及中西部的其他大学和行业)提供先进的仪器来满足俄亥俄州MS用户的需求。因此,该仪器也将提供给用户在俄亥俄州质谱社区。鉴于染色质结构在细胞生长和发育的适当调节中的核心作用,以及其作为癌症表观遗传疗法靶点的潜力,研究人员团队对确定组蛋白修饰的特定位点和模式感兴趣。由于组蛋白变体的同量异序性质和复杂的翻译后修饰模式,高分辨率质谱对组蛋白修饰的分析至关重要。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Investigation of the human tear film proteome using multiple proteomic approaches.
使用多种蛋白质组学方法研究人类泪膜蛋白质组。
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:2.2
- 作者:Green-Church,KariB;Nichols,KellyK;Kleinholz,NanM;Zhang,Liwen;Nichols,JasonJ
- 通讯作者:Nichols,JasonJ
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Michael A. Freitas其他文献
Determination of Relative Ordering of Activation Energies for Gas-Phase Ion Unimolecular Dissociation by Infrared Radiation for Gaseous Multiphoton Energy Transfer
通过红外辐射确定气态多光子能量转移的气相离子单分子解离活化能的相对有序度
- DOI:
10.1021/ja9925397 - 发表时间:
2000 - 期刊:
- 影响因子:15
- 作者:
Michael A. Freitas;and Christopher L. Hendrickson;A. Marshall - 通讯作者:
A. Marshall
Identification of a DNA methylation point in the promoter region of the bovine CYP21 gene.
牛 CYP21 基因启动子区域 DNA 甲基化点的鉴定。
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0.4
- 作者:
A. M. D. Silva;Michael A. Freitas;Á. Rios;A. Renzi;R. Lôbo;M. A. V. Galerani;R. A. Vila;E. S. Ramos - 通讯作者:
E. S. Ramos
Comparative Analysis Between Different Configurations in a Biomass Gasifier and a Genset for Electrical Energy Production
生物质气化炉和发电发电机组不同配置的比较分析
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
J. D. Silva;Francisco Everton Tavares de Luna;Vicente de Vasconcelos Claudino Filho;Michael A. Freitas - 通讯作者:
Michael A. Freitas
Techniques for estimating genetically variable peptides and semi-continuous likelihoods from massively parallel sequencing data.
从大规模并行测序数据估计遗传可变肽和半连续可能性的技术。
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
August E. Woerner;Benjamin Crysup;F. C. Hewitt;Myles W. Gardner;Michael A. Freitas;B. Budowle - 通讯作者:
B. Budowle
The applicability of fingernail lead and cadmium levels as subchronic exposure biomarkers for preschool children.
指甲铅和镉水平作为学龄前儿童亚慢性暴露生物标志物的适用性。
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:9.8
- 作者:
A. S. Oliveira;E. C. Costa;E. C. Pereira;Michael A. Freitas;B. Freire;B. L. Batista;M. Luz;K. P. Olympio - 通讯作者:
K. P. Olympio
Michael A. Freitas的其他文献
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{{ truncateString('Michael A. Freitas', 18)}}的其他基金
Identification of Histone Modification Interaction Networks
组蛋白修饰相互作用网络的鉴定
- 批准号:
7570472 - 财政年份:2009
- 资助金额:
$ 50万 - 项目类别:
Identification of Histone Modification Interaction Networks
组蛋白修饰相互作用网络的鉴定
- 批准号:
7897917 - 财政年份:2009
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modification in Cancer
癌症组蛋白修饰的筛选试验
- 批准号:
6941103 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modification in Cancer
癌症组蛋白修饰的筛选试验
- 批准号:
7115333 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modification in Cancer
癌症组蛋白修饰的筛选试验
- 批准号:
6762146 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modifications in Cancer
癌症组蛋白修饰的筛选分析
- 批准号:
8069229 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modification in Cancer
癌症组蛋白修饰的筛选试验
- 批准号:
7256926 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modifications in Cancer
癌症组蛋白修饰的筛选分析
- 批准号:
8490699 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别:
Assays for Screening Histone Modifications in Cancer
癌症组蛋白修饰的筛选分析
- 批准号:
8268528 - 财政年份:2004
- 资助金额:
$ 50万 - 项目类别: