Acquisition of a Next Generation Orbitrap MS

获得下一代 Orbitrap MS

• 批准号: 8640367
• 负责人: Michael A. Freitas
• 金额: $ 57.25万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640367
• 关键词: Calibration; Cells; Data Quality; Dissociation; Funding; Hybrids; Ions; Performance; Post Translational Modification Analysis; Process; Proteins; Proteomics; Request for Applications; Resolution; Sampling; Scanning; Source; Staging; Time; Tissue Sample; data acquisition; design; flexibility; human tissue; instrument; mass analyzer; next generation; novel; operation; research study; ultra high pressure

DESCRIPTION (provided by applicant): In this application we are requesting funds for the purchase of a ThermoScientific a next-generation Q-LTQ Orbitrap. We are requesting this instrument because its capabilities are well suited for bottom-up, middle-down and top-down protein characterization, high-sensitivity quantitative proteomics of limited samples and post- translational modification analysis. The requested next-generation hybrid Orbitrap has several key improvements over the current LTQ Orbitrap Elite that allow it to achieve better performance: 1) Mass selecting quadrupole available to select ions for Orbitrap or ion trap analysis, 2) Parallel operation of both ion trap and Orbitrap mass analyzers allowing for significantly increased scan rate, 3) HCD cell for ion accumulation in single ion or multiplexed modes, 4) Full flexibility of CID, HCD and ETD dissociations at any stage of MSn analysis, 5) Ultrahigh field Orbitrap with advanced transient processing, 6) Novel, high performance front-end ETD source and dedicated internal mass calibration. These improvements along with reduced overhead result in a high-resolution data acquisition rate greater than other designs without sacrificing sensitivity or dynamic range. The faster combined MS1 and MS2 scan rates of the requested next-gen Orbitrap are essential for LC- MS/MS with high efficiency ultra-high pressure LC separations, resulting in reduced proteomic experiment time without loss of data quality. This requested next-generation Q-LTQ Orbitrap will be critical to support the needs of several Major Users that performing large-scale proteomics on human tissue where samples are limited and sensitivity and throughput are key requirements.

描述（由申请人提供）：在本申请中，我们申请资金用于购买ThermoScientific下一代Q-LTQ Orbitrap。我们之所以需要这种仪器，是因为它的功能非常适合自下而上、自中而下和自上而下的蛋白质表征、有限样品的高灵敏度定量蛋白质组学和翻译后修饰分析。与当前的LTQ Orbitrap Elite相比，所要求的下一代混合Orbitrap有几项关键改进，使其能够实现更好的性能：1）质量选择四极可用于选择用于轨道阱或离子阱分析的离子，2）离子阱和轨道阱质量分析仪并行操作，允许显着提高扫描速率，3）HCD单元用于单离子或多路模式下的离子累积，4）在MSn分析的任何阶段CID、HCD和ETD解离的完全灵活性，5）具有先进瞬态处理的超高场Orbitrap，6）新型、高性能前端ETD源和专用内部质量校准。这些改进沿着降低的开销导致比其它设计更高的高分辨率数据采集速率，而不牺牲灵敏度或动态范围。所要求的下一代Orbitrap的更快的MS 1和MS 2扫描速率对于LC-MS/MS进行高效超高压LC分离至关重要，从而减少蛋白质组学实验时间而不损失数据质量。这一要求的下一代Q-LTQ Orbitrap对于支持几个主要用户的需求至关重要，这些用户对人体组织进行大规模蛋白质组学研究，其中样本有限，灵敏度和通量是关键要求。

项目成果

Concurrent renal amyloidosis and thymoma resulting in a fatal ventricular thrombus in a dog.

并发肾淀粉样变性和胸腺瘤导致狗致命的心室血栓。

• DOI: 10.1111/jvim.15062
• 发表时间: 2018
• 期刊: Journal of veterinary internal medicine
• 影响因子: 2.6
• 作者: Loewen,JenniferM;Cianciolo,RachelE;Zhang,Liwen;Yaeger,Michael;Ward,JessicaL;Smith,JodiD;LeVine,DanaN
• 通讯作者: LeVine,DanaN

Middle-Down Characterization of the Cell Cycle Dependence of Histone H4 Posttranslational Modifications and Proteoforms.

• DOI: 10.1002/pmic.201700442
• 发表时间: 2018-06
• 期刊: Proteomics
• 影响因子: 3.4
• 作者: Jiang T;Hoover ME;Holt MV;Freitas MA;Marshall AG;Young NL
• 通讯作者: Young NL

MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics.

• DOI: 10.1016/j.mcpro.2022.100256
• 发表时间: 2022-07
• 期刊: MOLECULAR & CELLULAR PROTEOMICS
• 影响因子: 7
• 作者: Johnson, Benjamin S.;Chafin, Lexie;Farkas, Daniela;Adair, Jessica;Elhance, Ajit;Farkas, Laszlo;Bednash, Joseph S.;Londino, James D.
• 通讯作者: Londino, James D.

The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent γ-butyrobetaine methyltransferase.

• DOI: 10.1016/j.jbc.2021.101327
• 发表时间: 2021-11
• 期刊: The Journal of biological chemistry
• 作者: Ellenbogen JB;Jiang R;Kountz DJ;Zhang L;Krzycki JA
• 通讯作者: Krzycki JA

Demonstration of In Vitro Resurrection of Aged Acetylcholinesterase after Exposure to Organophosphorus Chemical Nerve Agents.

暴露于有机磷化学神经毒剂后老化乙酰胆碱酯酶的体外复活演示。

• DOI: 10.1021/acs.jmedchem.7b01620
• 发表时间: 2018
• 期刊: Journal of medicinal chemistry
• 影响因子: 7.3
• 作者: Zhuang,Qinggeng;Franjesevic,AndrewJ;Corrigan,ThomasS;Coldren,WilliamH;Dicken,Rachel;Sillart,Sydney;DeYong,Ashley;Yoshino,Nathan;Smith,Justin;Fabry,Stephanie;Fitzpatrick,Keegan;Blanton,TravisG;Joseph,Jojo;Yoder,RyanJ;McElro
• 通讯作者: McElro