Acquisition of a Next Generation Orbitrap MS

描述（由申请人提供）：在此申请中，我们要求资金购买ThermoScientific下一代Q-LTQ Orbitrap。我们需要这台仪器，因为它的能力非常适合于自下而上，中向下和自上而下的蛋白质表征，有限样品的高灵敏度定量蛋白质组学和翻译后修饰分析。与目前的LTQ Orbitrap Elite相比，新一代混合Orbitrap有几个关键改进，使其能够实现更好的性能：1)质量选择四极杆可用于选择离子用于Orbitrap或离子阱分析，2)离子阱和Orbitrap质量分析仪的并行操作允许显著提高扫描速率，3)HCD电池用于单离子或多路模式的离子积累，4)在MSn分析的任何阶段充分灵活地进行CID， HCD和ETD解离，5)超高场Orbitrap具有先进的瞬态处理，6)新型，高性能前端ETD源和专用内部质量校准。这些改进以及减少的开销导致高分辨率数据采集率高于其他设计，而不会牺牲灵敏度或动态范围。新一代Orbitrap的更快的MS1和MS2联合扫描速率对于高效超高压LC分离的LC- MS/MS至关重要，从而在不损失数据质量的情况下减少蛋白质组学实验时间。这种要求的下一代Q-LTQ Orbitrap对于支持几个主要用户对人体组织进行大规模蛋白质组学的需求至关重要，这些用户的样品有限，灵敏度和吞吐量是关键要求。

DESCRIPTION (provided by applicant): In this application we are requesting funds for the purchase of a ThermoScientific a next-generation Q-LTQ Orbitrap. We are requesting this instrument because its capabilities are well suited for bottom-up, middle-down and top-down protein characterization, high-sensitivity quantitative proteomics of limited samples and post- translational modification analysis. The requested next-generation hybrid Orbitrap has several key improvements over the current LTQ Orbitrap Elite that allow it to achieve better performance: 1) Mass selecting quadrupole available to select ions for Orbitrap or ion trap analysis, 2) Parallel operation of both ion trap and Orbitrap mass analyzers allowing for significantly increased scan rate, 3) HCD cell for ion accumulation in single ion or multiplexed modes, 4) Full flexibility of CID, HCD and ETD dissociations at any stage of MSn analysis, 5) Ultrahigh field Orbitrap with advanced transient processing, 6) Novel, high performance front-end ETD source and dedicated internal mass calibration. These improvements along with reduced overhead result in a high-resolution data acquisition rate greater than other designs without sacrificing sensitivity or dynamic range. The faster combined MS1 and MS2 scan rates of the requested next-gen Orbitrap are essential for LC- MS/MS with high efficiency ultra-high pressure LC separations, resulting in reduced proteomic experiment time without loss of data quality. This requested next-generation Q-LTQ Orbitrap will be critical to support the needs of several Major Users that performing large-scale proteomics on human tissue where samples are limited and sensitivity and throughput are key requirements.