Shared Resource 14: Proteomics (PSR)

项目摘要-蛋白质组学共享资源(PSR) PSR为蛋白质组学实验提供专业知识和使用最先进的仪器。PSR 嵌入俄亥俄州立大学校园化学仪器中心，提供极具竞争力的价格和卓越的 通过利用研究和学院办公室的慷慨支持和NIH的赠款来提供服务。 在上次审查期间，PSR被评为分析共享资源组的杰出成员， 三个可寻址的评论以减少周转时间，实际由于以下原因导致用户百分比下降 作为OSU设施的非癌症用户的增加，以及感觉到的重复服务。主要服务包括：1) 为样品准备、MS实验设计、数据分析和时间表提供咨询；2)蛋白质鉴定 在各种样品矩阵上使用最先进的MS；3)深入的蛋白质表征，包括但不是 仅限于，翻译后修饰、蛋白质变体/突变、蛋白质截断位点检测、替代 剪接形式检测、从头蛋白质测序、蛋白质交联和蛋白质-蛋白质相互作用；4) 使用无标记(光谱计数和/或相对强度)或稳定同位素标记技术进行蛋白质定量 (SILAC或iTRAQ/TMT)、定量MALDI组织成像和靶向质谱学；以及5)数据 使用商业和内部开发的软件平台随BISR提供的分析。主要装备 在过去5年中，通过三笔NIH S10赠款和一个资助的P41资源中心 方法发展。PSR的具体目标是：1)提供先进的基于质谱学的蛋白质组学 服务；2)提供创新的蛋白质组数据分析和生物信息学平台，方便用户 3)提供自营MS仪器的实验设计和培训咨询。 在本供资周期内，PSR为支持44种出版物(5&>10种影响)提供了关键服务 因素)，380个用户，8个NCI授权，包括1个K22，1个P01，4个R01，1个R33和1个U01。PSR对以下方面至关重要 OSUCCC的免疫肿瘤学、翻译基因组学、癌症工程和癌症的研究重点 预防和生存。先进的分析平台使研究人员能够以不同的方式发现新事物 血清、尿液、BAL液、唾液、冰冻组织、福尔马林固定组织、细胞培养液、 和细胞裂解物。鉴于OSUCCC招聘情况强劲，对服务和新技术的需求将 增加。在达到能力之前，GSR将扩大其工作人员、仪器和服务。致信地址 这项计划中的新服务正在实施或扩展(使用BISR的信息学服务、毛细服务 已开发的使用天然MS的电泳法和离子迁移率方法、组织成像和结构生物学 由P41资源赠款)。在下一个资助期，PSR将成为免疫监测的成员 和探索平台。PSR的年度预算是1,993,101美元，而协调小组的请求是158,381美元。因此， PSR利用了广泛的机构支持，仅从CCSG基金寻求7.9%的支持。

PROJECT SUMMARY – PROTEOMICS SHARED RESOURCE (PSR) The PSR provides expertise and access to state-of-the-art instrumentation for proteomics experiments. The PSR is embedded within the OSU Campus Chemical Instrument Center, offering competitive pricing and exceptional service by leveraging generous support from the Office of Research and Colleges, and grants from the NIH. During the last review, the PSR was rated Outstanding as part of the Analytics Shared Resource Group, with three addressable comments to reduce turn-around time, a perceived drop in percent users actually due to an increase of non-cancer users as an OSU facility, and perceived duplicative services. Major services include: 1) consultation for sample preparation, MS experiment design, data analysis, and timelines; 2) protein identification using state-of-the-art MS on a variety of sample matrices; 3) in depth protein characterization including, but not limited to, post-translational modifications, protein variant/mutations, protein truncation sites detection, alternate splice form detection, de novo protein sequencing, protein cross-linking, and protein-protein interactions; 4) protein quantification using label-free (spectral counts and/or relative intensity) or stable isotope label techniques (SILAC or ITRAQ/TMT), quantitative MALDI tissue imaging, and targeted mass spectrometry; and 5) data analysis provided with the BISR using commercial and in-house developed software platforms. Major equipment has been enhanced in the past 5 years through three NIH S10 grants and a funded P41 Resource Center for methods development. PSR Specific Aims are to: 1) provide advanced mass spectrometry-based proteomics services; 2) provide innovative proteomic data analytics and bioinformatics platforms to facilitate user interpretation; and, 3) provide consultation on experimental design and training on self-operated MS instruments. Over the current funding cycle, the PSR provided key services in support of 44 publications (5 > 10 impact factor), 380 users, and 8 NCI grants, including 1 K22, 1 P01, 4 R01s, 1 R33 and 1 U01. The PSR is critical to the OSUCCC research priorities of immuno-oncology, translational genomics, cancer engineering and cancer prevention and survivorship. Advanced analytical platforms allow researchers to discover novel differentially expressed proteins in serum, urine, BAL fluid, saliva, frozen tissues, formalin fixed tissues, cell culture media, and cell lysates. Given the robust OSUCCC recruitment, demand for services and new technologies will increase. The GSR will expand its staff, instrumentation and services before capacity is reached. To address this, planned new services are being implemented or expanded (informatics services with BISR, capillary electrophoresis and ion mobility methods, tissue imaging, and structural biology using native MS, as developed by a P41 Resource grant). Over the next funding period, the PSR will be a member of the Immune Monitoring and Discovery Platform. The annual budget of the PSR is $1,993,101, yet the CCSG request is $158,381. Thus, the PSR leverages extensive institutional support and seeks only 7.9% support from CCSG funds.