Microbial Production Core Facility

描述（由申请方提供）：我们拟购买一台新玩法Scientific BIOFLO 5000 60 L工作体积发酵罐和一台CEPA Z41高容量流通式离心机，以建立微生物生产核心设施，主要用于表达重组蛋白的细菌和酵母菌株的生长。拟议的核心设施将满足15名NIH支持的研究人员的需求，这些研究人员通过34个不同的NIH奖项（22个R01，1个R21，1个R33，2个R37（MERIT），2个P01，1个P30，1个P41，1个S10，1个T32，1个U19，1个U54）获得资助。核心设施将克服所有相关实验室在蛋白质纯化方面的重大瓶颈。参与这项申请的研究人员研究了130种不同的蛋白质，通常是野生型和突变型，总共代表了200多种蛋白质制剂。计划中的核心设施在培养大型培养物方面比现有的可能性（10升发酵罐，烧瓶）更有效，允许更大的蛋白质制备，并将提高起始细胞材料的重现性。将为每个参与的研究计划节省大量时间。起始材料量的飞跃将允许以足够的浓度纯化制备量的难溶性蛋白质，以最终允许它们的生物化学和生物物理分析。我们选择新玩法Scientific BIOFLO 5000和CEPA Z41是基于之前新玩法发酵罐和CEPA产品的经验、运行类似设施的个人的输入、价格和仪器的规格。相关性：本次提交的研究人员从事基础和应用生物医学研究，使用纯化的蛋白质来定义与癌症和人类健康相关的机制。所要求的仪器不仅使他们的工作效率显著提高，而且还允许分析以前无法以足够的数量或浓度纯化的蛋白质。

DESCRIPTION (provided by applicant): We propose to purchase a New Brunswick Scientific BIOFLO5000 60 liter working volume fermentor and a CEPA Z41 high capacity flow-through centrifuge to establish a Microbial Production Core Facility primarily for the growth of bacterial and yeast strains expressing recombinant proteins. The proposed core facility will serve the needs of 15 NIH-supported investigators, which are funded through 34 different NIH awards (22 R01, 1 R21, 1 R33, 2 R37 (MERIT), 2 P01, 1 P30, 1 P41, 1 S10, 1 T32, 1 U19, 1 U54). The core facility will overcome a significant bottleneck in protein purification for all laboratories involved. The investigators involved in this application work on 130 different proteins, often in their wild type and mutant forms, representing a total of over 200 protein preparations. The planned core facility is more efficient in growing large cultures than existing possibilities (10 liter fermentor, flasks), allows larger protein preparations, and will enhance the reproducibility of the starting cell material. Significant time savings for each participating research program will result. The leap in the amount of starting material will allow purifying preparative amounts of poorly soluble proteins at sufficient concentrations to finally allow their biochemical and biophysical analysis. Our choice of the New Brunswick Scientific BIOFLO5000 and the CEPA Z41 is based on previous experience with New Brunswick fermentors and CEPA products, the input from individuals running similar facilities, the pricing, and the specifications of the instruments. Relevance: The investigators of this submission engage in fundamental and applied biomedical research, using purified proteins to define mechanisms that are relevant to cancer and human health. The requested instrumentation will not only make their work significantly more efficient, but will also allow the analysis of proteins that previously could not be purified in sufficient quantities or concentrations.