Purchase of a 500 MHz Solid State NMR Spectrometer

购买 500 MHz 固态核磁共振波谱仪

• 批准号: 7214442
• 负责人: Ayyalusamy Ramamoorthy
• 金额: $ 50万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-15 至 2009-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214442
• 关键词: Biological; Biomedical Research; Biophysics; Chemistry; Communities; Dendrimers; Dental; Development; Funding; Grant; Institution; Interdisciplinary Study; Investigation; Medical; Membrane; Membrane Proteins; Michigan; NMR Spectroscopy; Peptide Antibiotics; Pharmaceutical Preparations; Pharmacologic Substance; Research Personnel; Research Project Grants; Resolution; Structure; System; Therapeutic; United States National Institutes of Health; Universities; bone; instrument; solid state

DESCRIPTION (provided by applicant): We propose to purchase a 500 MHz solid-state NMR (SSNMR) spectrometer that will be used for biomedical research by the Biophysics, Chemistry, Dental and Medical communities at the University of Michigan. At the University of Michigan, researchers utilize SSNMR spectroscopy to obtain high-resolution information about the structure, dynamics, geometry and interactions of non-soluble and/or non-crystalline biological systems. Progress of these NIH-funded research projects is hampered due to lack of a sophisticated high field SSNMR spectrometer. Therefore, this purchase will significantly extend the applicability of SSNMR spectroscopy to research projects that are presently under study at our institution and nucleate new initiatives in interdisciplinary research. The 500 MHz spectrometer will be used to study the high-resolution structure of membrane-associated proteins, regulatory RNAs that are important targets for development of anti-HIV therapeutics, and polymorphic pharmaceutical drugs. In addition, investigation of bone materials and membrane interaction of peptide antibiotics and dendrimers will significantly benefit from the use of the spectrometer. All the major users of the instrument are supported by NIH R01 grants.

描述(由申请人提供)：我们建议购买一台500 MHz固态核磁共振(SS核磁共振)光谱仪，用于密歇根大学生物物理、化学、牙科和医学界的生物医学研究。 在密歇根大学，研究人员利用SS核磁共振光谱学获得关于不可溶和/或非晶体生物系统的结构、动力学、几何和相互作用的高分辨率信息。由于缺乏先进的高场SSNMR谱仪，这些由NIH资助的研究项目的进展受到阻碍。因此，此次收购将极大地扩大SS核磁共振波谱的适用范围，使其适用于我们机构目前正在研究的研究项目，并在跨学科研究中形成新的倡议。 500 MHz光谱仪将用于研究膜相关蛋白、调控RNA的高分辨率结构，这些RNA是抗HIV治疗药物开发的重要目标，以及多态药物。此外，多肽抗生素和树枝状大分子的骨材料和膜相互作用的研究将从光谱仪的使用中受益匪浅。 该仪器的所有主要用户都得到了NIH R01拨款的支持。

项目成果

Solid-state NMR spectroscopy provides atomic-level insights into the dehydration of cartilage.

固态核磁共振波谱提供了软骨脱水的原子级见解。

• DOI: 10.1021/jp205663z
• 发表时间: 2011
• 期刊: The journal of physical chemistry. B
• 作者: Xu,Jiadi;Zhu,Peizhi;Morris,MichaelD;Ramamoorthy,Ayyalusamy
• 通讯作者: Ramamoorthy,Ayyalusamy

Development of bifunctional stilbene derivatives for targeting and modulating metal-amyloid-β species.

• DOI: 10.1021/ic2012205
• 发表时间: 2011-11-07
• 期刊: Inorganic chemistry
• 影响因子: 4.6
• 作者: Braymer JJ;Choi JS;DeToma AS;Wang C;Nam K;Kampf JW;Ramamoorthy A;Lim MH
• 通讯作者: Lim MH

Membrane disruption and early events in the aggregation of the diabetes related peptide IAPP from a molecular perspective.

• DOI: 10.1021/ar200189b
• 发表时间: 2012-03-20
• 期刊: ACCOUNTS OF CHEMICAL RESEARCH
• 影响因子: 18.3
• 作者: Brender, Jeffrey R.;Salamekh, Samer;Ramamoorthy, Ayyalusamy
• 通讯作者: Ramamoorthy, Ayyalusamy

NMR characterization of monomeric and oligomeric conformations of human calcitonin and its interaction with EGCG.

• DOI: 10.1016/j.jmb.2011.12.023
• 发表时间: 2012-02-10
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Huang, Rui;Vivekanandan, Subramanian;Brender, Jeffrey R.;Abe, Yuki;Naito, Akira;Ramamoorthy, Ayyalusamy
• 通讯作者: Ramamoorthy, Ayyalusamy

Variable reference alignment: an improved peak alignment protocol for NMR spectral data with large intersample variation.

• DOI: 10.1021/ac301327k
• 发表时间: 2012-06-19
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: MacKinnon, Neil;Ge, Wencher;Khan, Amjad P.;Somashekar, Bagganahalli S.;Tripathi, Pratima;Siddiqui, Javed;Wei, John T.;Chinnaiyan, Arul M.;Rajendiran, Thekkelnaycke M.;Ramamoorthy, Ayyalusamy
• 通讯作者: Ramamoorthy, Ayyalusamy