Essential Human Embryonic Stem Cell Culture Methods

描述（由申请人提供）：本提案概述了在明尼苏达大学干细胞研究所建立人类胚胎干细胞（hESC）培训计划的计划。该计划旨在支持在实验室开展广泛的研究课题的hESC研究。该项目借鉴了UM干细胞研究所在干细胞研究方面的深度经验，特别是hESC研究。基于该项目教师的丰富经验，以及来自UM干细胞研究所和外部演讲者的客座参与者，该项目的参与者将获得hESC培养所需的基本程序的实践经验，并在积极从事干细胞和hESC研究的科学家的讲座中学习启动hESC研究的策略。该计划包括三个部分，包括为期三天的培训计划，将在UM干细胞研究所的新址进行。全年开设四门课程，每门课程将选出五名学员。课程的主要重点是准备和使用饲料培养和hESC的实践培训。主要使用hESC线UC06和WI09，但线UC01， WI01, MI01, ES01， ES03和ES04也可用于演示。该课程还包括讲授分化、基因改造和银行策略等内容。最后，来自明尼苏达大学干细胞研究所的客座讲师和来自明尼苏达大学以外研究界的嘉宾将介绍干细胞的研究和临床应用。小班授课将使我们能够根据学员的经验水平和研究兴趣给予个性化的关注。课程每年提供四次，将为参与者提供灵活的日程安排。参与者的持续评估和项目教师的后续支持将为改进后续课程提供灵活性。该项目的教师在成功培养hESC技术研究人员方面拥有超过5年的丰富经验。该计划为干细胞研究界建立了一个宝贵的资源。该提案概述了建立人类胚胎干细胞（hESC）用于研究的培训计划的计划。该项目将培训研究人员将hESC研究纳入其实验室正在进行的研究中。该计划包括实验室演示，hESC使用讲座，以及hESC研究在生物学和疾病治疗中的当前和未来应用的客座讲座。

DESCRIPTION (provided by applicant): This proposal outlines a plan to establish a human embryonic stem cell (hESC) training program at the Stem Cell Institute at the University of Minnesota. The program is designed to support the establishment of hESC studies in laboratories undertaking a broad range of research topics. The program draws upon of the depth of experience in stem cell research, and specifically hESC research at the UM Stem Cell Institute. Based on the extensive experience of the faculty of the program, and guest participants both from the UM Stem Cell Institute and outside speakers, the participants in the program will gain hands-on experience in the essential procedures required for hESC culture, and learn strategies for initiating hESC research in lectures presented by scientists actively engaged in stem cell and hESC research. The program consists of three sections, covered in a three-day training program to take place at the new site of the UM Stem Cell Institute. Five participants will be chosen for each of the four courses offered throughout the year. The main focus of the course is hands-on training on the preparation and use of feeder cultures and hESC. The hESC lines UC06 and WI09 will primarily be used, but lines UC01, WI01, MI01, ES01, ES03 and ES04 are also available for demonstration. The program also includes lecture sections covering differentiation, genetic modification and banking strategies. Finally, guest lecturers from the UM Stem Cell Institute, and guests from the research community outside of the University of Minnesota will describe research and clinical applications for stem cells. The small size of the classes will allow us to give individual attention to the participants' level of experience and research interests. Classes offered four times per year will provide participants flexibility in scheduling. Ongoing evaluation by the participants, and follow-up support by the program faculty will provide flexibility in refining subsequent classes. The faculty of this program have extensive experience in successfully training researchers in hESC technology for more than 5 years. This program establishes a valuable resource for the stem cell research community. This proposal outlines a plan to establish a training program in the use of human embryonic stem cells (hESC) for research. The program will train researchers to incorporate hESC studies into the research ongoing in their labs. The program consists of laboratory demonstrations, lectures on the use of hESC, and guest lectures on current and future applications of hESC research for biology and disease treatments.