Indiana Gene Array Core

印第安纳基因阵列核心

• 批准号: 7214467
• 负责人: HOWARD J EDENBERG
• 金额: $ 17.41万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214467
• 关键词: Drosophilidae; alcoholism /alcohol abuse; amygdala; biochemistry; bioinformatics; biomedical facility; cooperative study; experimental designs; gene expression profiling; genetic models; laboratory rat; microarray technology; neurogenetics; nucleic acid purification; polymerase chain reaction

DESCRIPTION (provided by applicant): The overall aim of the Indiana Gene Array Core is to collaborate with INIA investigators to understand the differences in gene expression in the extended amygdala that are involved in the development of excessive drinking. Our hypothesis is that both innate differences in gene expression and differences in response to alcohol and to paradigms involving exposure to alcohol or its withdrawal (gene-environment interactions) contribute to the development of excessive alcohol drinking. The Indiana Gene Array Core will focus on the use of Affymetrix microarrays to measure gene expression in selected regions of rat brains and in Drosophila mutants after various treatments; primary collaborators will be McBride (U01), Ryabinin (U01) and Davis (U01). These studies build upon our work during the initial grant period; carrying out the follow-up studies in the same laboratory (with many of the same technical personnel) will reduce technical variations and allow more powerful metaanalyses. We will work with investigators on experimental design, extract and purify RNA from rat brain tissues provided by the collaborating investigators, carry out all of the quality control and biochemistry involved in GeneChip experiments, extract the data and assist with analyses and bioinformatics. This core will be housed in the Center for Medical Genomics, directed by the PI, which has all of the necessary equipment and carries out a high volume of Affymetrix GeneChip experiments with a focus on quality control. We will extend our services to include quantitative RT-PCR confirmation of key findings from the microarrays that we process, and will offer such services to other members of INIA. We will also provide members of INIA analyses of cis-acting elements that contribute to the differences in gene expression detected in the microarray experiments; we have developed model-based techniques that do not require prior knowledge of binding sites. This Core will provide high quality data in an efficient and economical way. We already have strong relatioinships with the users, which will benefit the overall project.

描述（由申请人提供）：印第安纳基因阵列核心的总体目的是与INIA研究人员合作，以了解与饮用过量饮酒有关的扩展杏仁核中基因表达的差异。我们的假设是，基因表达的先天差异和对酒精反应的差异和涉及暴露于酒精的范例或其戒断（基因环境相互作用）都有助于发展过量酒精。印第安纳基因阵列核心将集中于使用Affymetrix微阵列在各种处理后的大鼠大脑和果蝇突变体中选定区域的基因表达。主要合作者将是McBride（U01），Ryabinin（U01）和Davis（U01）。这些研究基于我们在最初的赠款期间的工作；在同一实验室（与许多同一技术人员一起）进行后续研究将减少技术差异，并允许更强大的荟萃分析。我们将与研究人员合作进行实验设计，从合作研究者提供的大鼠脑组织中提取和纯化RNA，进行参与Genechip实验的所有质量控制和生物化学，提取数据并协助分析和生物信息学。该核心将安置在由PI指导的医学基因组中心中，该中心具有所有必要的设备，并进行了大量的Affymetrix Genechip实验，重点是质量控制。我们将扩展我们的服务，以包括对我们处理的微阵列中关键发现的定量RT-PCR确认，并将为INIA的其他成员提供此类服务。我们还将提供从微阵列实验中检测到的基因表达差异的顺式作用元件的INIA分析成员。我们已经开发了基于模型的技术，这些技术不需要绑定位点的先验知识。该核心将以有效，经济的方式提供高质量的数据。我们已经与用户拥有强大的关系启动，这将使整个项目受益。