Applied Biosystems SOLiD 3 System

描述（由申请人提供）：此共享仪器补助金是购买应用生物系统SOLiD 3系统的高通量DNA测序。高通量测序对印第安纳州大学医学院正在进行的许多研究项目非常重要，这些项目研究了酗酒、帕金森病、骨质疏松症和其他影响数百万人的主要疾病的遗传基础。这项技术对于发现与这些疾病相关的功能变体，分析转录组和调控micro-RNA以了解作用机制，分析转录因子结合和组蛋白修饰（ChIP-Seq）的DNA-蛋白质相互作用，检查DNA甲基化及其在疾病中的作用以及研究疾病中的肠道或口腔生物群系非常重要。因此，该文书将大大有助于我们在了解常见疾病方面取得进展。

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant is to purchase an Applied Biosystems SOLiD 3 System for high-throughput DNA sequencing. High-throughput sequencing is important to many ongoing research projects at the Indiana University School of Medicine examining the genetic basis of alcoholism, Parkinson disease, osteoporosis, and other major diseases that affect millions of people. This technology is important to find the functional variants associated with these diseases, to analyze the transcriptome and regulatory micro-RNAs to understand mechanisms of action, to analyze DNA-protein interactions both for transcription factor binding and for histone modifications (ChIP-Seq), to examine DNA methylation and its role in disease, and to study the gut or oral biome in disease. The instrument will, therefore, contribute greatly to progress in our understanding of common diseases.