Applied Biosystems SOLiD 3 System

Applied Biosystems SOLiD 3 系统

• 批准号: 7793824
• 负责人: HOWARD J EDENBERG
• 金额: $ 49.24万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793824
• 关键词: Affect; Alcoholism; Binding; DNA Methylation; DNA Sequence; DNA-Protein Interaction; Disease; Gene Expression Profile; Genetic; Grant; Indiana; MicroRNAs; Oral; Osteoporosis; Parkinson Disease; Research Project Grants; Role; Solid; System; Technology; Universities; Variant; base; histone modification; instrument; instrumentation; medical schools; transcription factor

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant is to purchase an Applied Biosystems SOLiD 3 System for high-throughput DNA sequencing. High-throughput sequencing is important to many ongoing research projects at the Indiana University School of Medicine examining the genetic basis of alcoholism, Parkinson disease, osteoporosis, and other major diseases that affect millions of people. This technology is important to find the functional variants associated with these diseases, to analyze the transcriptome and regulatory micro-RNAs to understand mechanisms of action, to analyze DNA-protein interactions both for transcription factor binding and for histone modifications (ChIP-Seq), to examine DNA methylation and its role in disease, and to study the gut or oral biome in disease. The instrument will, therefore, contribute greatly to progress in our understanding of common diseases.

描述(申请人提供)：这项共享仪器拨款是为了购买一台应用生物系统Solid 3系统，用于高通量DNA测序。高通量测序对于印第安纳大学医学院正在进行的许多研究项目非常重要，这些项目旨在研究酒精中毒、帕金森病、骨质疏松症和其他影响数百万人的重大疾病的遗传基础。这项技术对于发现与这些疾病相关的功能变异，分析转录组和调节microRNAs以了解作用机制，分析DNA-蛋白质相互作用的转录因子结合和组蛋白修饰(ChIP-Seq)，检查DNA甲基化及其在疾病中的作用，以及研究肠道或口腔生物组在疾病中的作用是重要的。因此，该文书将极大地促进我们对常见疾病的了解。