Neuron-specific modulations of vertebrate myelination and their effects on neuronal function

最近在啮齿类动物和斑马鱼中使用强大的遗传操作的研究表明，髓鞘形成依赖于神经元活动，尽管我们仍然不知道髓鞘形成如何影响不同神经元的功能。在发育过程中，不同的髓鞘形成模式取决于轴突的几何形状和神经元的身份。目前还没有办法在一个完整的中枢神经系统中以神经元特异性的方式抑制髓磷脂。我的项目旨在开发这些方法，并研究神经元的功能和活力。我将用转基因模型，特别是斑马鱼和老鼠，来改变目标基因的表达。一旦技术成熟，我将评估受影响神经元的形态、生存能力和生理活动。

Recent studies using powerful genetic manipulations in rodents and zebrafish showed that myelination is dependent on neuronal activity although we still don't know how myelination affects the function of different neurons. During development, different patterns of myelination arise that depend on axonal geometry and neuronal identity. There are currently no methods to inhibit myelin in a neuronal-specific way in an otherwise intact CNS. My project aims to develop such methods and to study neuronal function and viability. I will do so with genetically modified models, specifically in zebrafish and mice, to modify the expression of the target genes. Once the techniques are developed, I will assess the morphology, viability, and physiological activity of the affected neurons.