GEISHA, a Chicken Embryo Gene Expression Resource

随着人类基因组测序的完成，人们的注意力集中在了解基因功能上， 对于大约一半的基因来说，这一点仍然未知。关键的第一步是确定空间和 基因表达的时间模式。这对于胚胎发育的早期阶段尤其重要，当 当对正常发育至关重要的基因表达时，许多重要的发育过程就会发生。 由于人类胚胎中的基因表达分析在伦理上是站不住脚的，因此表达模式的映射必须 在模型生物体中完成。鸡是首屈一指的非哺乳动物模式生物，用于 在广泛的研究学科中进行研究。鸡胚为人类提供了许多优势 大规模的表情筛选，包括惊人的相似性，低成本和现成的可获得性。 这项建议的目标是创建一个鸡胚胎原位杂交表达资源。 目标如下：1)扩大现有的基因表达筛选工作，称为艺妓 (Gallus Est原位杂交分析)，使用自动化高通量整体原位杂交 通过四天的发育分析定位鸡胚胎中表达序列的位置；2) 我通过平行的协调筛选工作，从个别研究人员那里获得更多的表达数据， 3)将表情信息存储在关系数据库中，并呈现表情 使用可免费访问的网络界面将数据提供给研究社区。鸡的解剖本体论将是 创建用于描述表达模式并启用时空查询，数据将用于 对鸡的基因组进行注释。这个集中的原位杂交基因表达信息库 将为生物医学研究界提供宝贵和全面的资源。

With sequencing of the human genome completed, attention is focusing on understanding gene function, which for approximately half of all genes remains unknown. A crucial first step is to determine spatial and temporal patterns of gene expression. This is especially important for early stages of embryogenesis, when many important developmental processes occur and when genes critical for normal development are expressed. Since gene expression analysis in human embryos is ethically untenable, mapping of expression patterns must be accomplished in model organisms. Chicken is the premiere non mammalian model organism, and is used in research across a broad spectrum of research disciplines. The chicken embryo provides many advantages for large-scale expression screening, including striking similarities to human, low expense and ready availability. The goal of this proposal is to create an in situ hybridization expression resource for chicken embryos. The objectives are as follows: 1) to expand an existing gene expression screening effort, called GEISHA (gallus est in situ hybridization analysis), using automated high throughput whole mount in situ hybridization analysis to map the location of expressed sequences in chick embryos through four days of development; 2) to iacquire additional expression data through parallel coordinated screening efforts, from individual researchers, and from published results; 3) to house expression information in a relational database and present expression data to the research community using a freely accessible web interface. A chicken anatomical ontology will be created to describe expression patterns and to enable spatio-temporal querying, and data will be used to annotate the chicken genome. This centralized repository for in situ hybridization gene expression information will provide a valuable and comprehensive resource for the biomedical research community.