Tech Development Core

技术发展的核心是扩大高质量的原位杂交 (ISH)目前可通过艺妓(Gallus Expression In Site)获得的表情数据集 杂交分析)数据库和用户界面，并将艺妓数据集成到AviPort。艺妓 是一个成熟的鸡胚胎社区资源，提供ISH基因表达 信息和相关基因组元数据通过内部ISH屏幕、文献管理和 从其他研究实验室获取表达数据。近年来，艺妓项目已经 为以下项目生成了包含50,000个完整装载ISH图像和关联元数据的高质量数据集 大约3200个发育调节基因。与许多生物医学相关的鸟类的焦点 研究发育的分子调控和直接实验分析，优质ISH 信息对鸟类研究界具有特别高的价值。尽管在过去的几年里，艺妓 已经是一个独立的资源，未来艺妓将整合到AviPort的高吞吐量中 将继续进行ISH筛查，因为该数据已被投票为最高优先级， 为鸟类研究界提供高质量的基因组。其他ISH信息将从 文献，并从其他研究人员那里获得。与Curation Core合作，一只鸡的解剖 将创建本体并将其用于标注ISH鸡胚图像。

The goal of the Technology Development Core is to expand the high quality in situ hybridization (ISH) expression dataset that is presently available through the GEISHA (gallus expression in situ hybridization analysis) database and user interface, and to integrate GEISHA data into AviPort. GEISHA is an established community resource for chicken embryo, providing ISH gene expression information and associated genomic metadata through in house ISH screens, literature curation and acquisition of expression data from other research laboratories. In recent years, the GEISHA project has generated a high quality dataset of 50,000 whole mount ISH images and associated metadata for approximately 3,200 developmentally regulated genes. With the focus of much biomedically related avian research on the molecular regulation of development and direct experimental analysis, high quality ISH information is of particularly high value to the avian research community. Though in past years GEISHA has been a stand-alone resource, going forward GEISHA will be integrated into AviPort High throughput ISH screens will continue to be conducted as this data has been voted the highest priority, after high quality genomes, for the avian research community. Additional ISH information will be curated from the literature and acquired from other researchers. In collaboration with the Curation Core, a chicken anatomical ontology will be created and used to annotate ISH chicken embryo images.