GEISHA: A Chicken Embryo Gene Expression Resource

描述（申请人提供）：生物医学研究的一个中心范式是，了解基因功能将最终导致人类健康的改善。 关键的第一步是要知道基因在胚胎发育和成体中的表达位置和时间。 鸡胚为大规模表达筛选提供了许多优势，包括与人类惊人的相似性，低成本和作为实验模式生物的悠久历史。 此次更新申请的目的是继续支持和扩展GEISHA（鸡表达原位杂交分析）基因表达数据库，这是一个已建立的鸡胚原位杂交基因表达信息和相关基因组元数据的社区资源。 本申请的具体目的是（1）用靶向高通量原位杂交筛选、科学文献和其他研究人员提供的鸡基因表达信息填充GEISHA数据库。 将进行高通量原位杂交分析，以确定发育调控基因的表达模式。 原位杂交图像和相关信息也将从出版物中收集，并从研究人员那里获得。 2)GEISHA数据库将扩大，以包括新类型的信息和特征。 原位杂交图像和相关数据将与其他基因表达项目交换，以使跨物种的表达信息在每个平台上本地显示。 BioMart数据集成系统和出版物管理系统也将被添加，以增强搜索和检索能力，并显示基因相关的出版物。 3)用户界面将得到改进，以容纳新的功能并方便导航。 基因表达报告页面将被重新组织和扩展，以包括详细的基因摘要信息，基因相关的出版物，以及多个物种的基因表达信息。 用户友好的查询功能将扩展到包括OMIM人类疾病基因和基因本体论术语的搜索。 通过这些改进，GEISHA将继续为生物医学研究提供宝贵和全面的资源。 相关性：该项目的目标是增强鸡胚胎的重要现有基因表达资源，鸡胚胎是公认的人类疾病生物医学模型。 这些服务将提供有价值的信息，有助于在各种相关领域努力查明人类疾病的潜在机制。

DESCRIPTION (Provided by Applicant): A central paradigm of biomedical research is that understanding gene function will ultimately lead to improvements in human health. A crucial first step is to know where and when genes are expressed during embryogenesis and in the adult. The chicken embryo provides many advantages for large-scale expression screening, including striking similarities to human, low expense, and a long history as an experimental model organism. The goal of this renewal application is to continue support and expansion of the GEISHA (gallus expression in situ hybridization analysis) gene expression database, an established community resource for chicken embryo in situ hybridization gene expression information and associated genomic metadata. Specific aims of this application are (1) to populate the GEISHA database with chicken gene expression information arising from targeted high throughput in situ hybridization screens, from the scientific literature, and from other researchers. High throughput in situ hybridization analyses will be performed to determine the expression patterns of developmental regulatory genes. In situ hybridization images and associated information will also be curated from publications and obtained from researchers. 2) The GEISHA database will be expanded to encompass new types of information and features. In situ hybridization images and associated data will be exchanged with other gene expression projects to enable cross-species display of expression information locally on each platform. The BioMart data integration system and a publication management system will also be added to enhance search and retrieval capabilities and to display gene related publications. 3) The user interface will be enhanced to accommodate new features and to facilitate navigation. Gene Expression Report pages will be reorganized and expanded to include detailed gene summary information, gene-related publications, and gene expression information for multiple species. User-friendly query features will be expanded to include searching by OMIM human disease gene and Gene Ontology terms. Through these enhancements, GEISHA will continue to provide a valuable and comprehensive resource for biomedical research. RELEVANCE: The objective of this project is to enhance an important existing gene expression resource for the chicken embryo, a recognized biomedical model of human disease. These services will provide valuable information that will assist efforts across a wide variety of related fields towards the identification of mechanisms underlying human diseases.