ANALYSIS OF GENETICALLY & ENVIRONMENTALLY OVERLAPPING YEAST QUANTITATIVE TRAITS

描述(由申请人提供)：广泛的长期目标：这项工作的广泛的长期目标是提高我们对数量性状/遗传学和遗传多样性的理解，重点是酿酒酵母中特定的数量性状。更好地理解数量性状需要更好地理解转录/翻译调节、多效性、表型异质性和基因-环境相互作用，所有这些都在本提案中讨论。具体目标：本工作的具体目标1是确定酿酒酵母数量性状基因座中控制高温生长、线粒体基因组维持、孢子形成和絮凝的数量性状基因/核苷酸。这项工作的具体目标2是确定我们的两个高温生长数量性状基因MKT1和SSD1的靶标和互作因子，它们是翻译调节基因。这项工作的具体目标3是评估我们的数量性状基因在多种遗传背景下的贡献。相关性：对数量性状的理解需要更好地理解转录/翻译调节、多效性、表型异质性和基因-环境相互作用。这个项目与健康的关系是双重的。首先，人类的大多数基于基因的疾病负担在本质上是量化的。因此，对酵母等简单真核生物数量性状的更好理解将有助于理解人类的数量性状。其次，酿酒酵母是一种新出现的机会致病菌，毒力是一个数量性状。因此，对数量性状的更好理解将有助于我们理解无害的共生/腐生酵母是如何成为一种机会性病原体的。

DESCRIPTION (provided by applicant): Broad long-term objectives: The broad long-term objectives of this work are to improve our understanding of quantitative traits/genetics and genetic diversity, focusing on specific quantitative traits in S. cerevisiae. Improved understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions, all of which are addressed in this proposal. Specific Aims: Specific Aim 1 of this work is to identify in S. cerevisiae quantitative trait loci, quantitative trait genes/nucleotides controlling high temperature growth, mitochondrial genome maintenance, sporulation and flocculation. Specific Aim 2 of this work is to identify the targets and interactors of two of our high temperature growth quantitative trait genes, MKT1 and SSD1 that are translational regulators. Specific Aim 3 of this work is to assess the contributions of our quantitative trait genes in multiple genetic backgrounds. Relevance: The understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions. The health relatedness of this project is two-fold. First, most of the genetically based disease burden in humans is quantitative in nature. Therefore, an improved understanding of quantitative traits in a simple eukaryote such as yeast will aid the understanding of quantitative traits in humans. Second, S. cerevisiae is an emerging opportunistic pathogen and virulence is a quantitative trait. Therefore, an improved understanding of quantitative traits will aid our understanding of how a harmless commensal/saprophytic yeast can become an opportunistic pathogen.