ANALYSIS OF GENETICALLY & ENVIRONMENTALLY OVERLAPPING YEAST QUANTITATIVE TRAITS

广泛的长期目标：这项工作的广泛的长期目标是提高我们对数量性状/遗传学和遗传多样性的理解，重点是S.啤酒。提高对数量性状的理解需要更好地理解转录/翻译调控，多效性，表型异质性和基因-环境相互作用，所有这些都在这个建议中得到解决。具体目标：本工作的具体目标1是确定在S。酿酒酵母数量性状基因座、控制高温生长的数量性状基因/核苷酸、线粒体基因组维持、孢子形成和絮凝。本工作的具体目标2是确定我们的两个高温生长数量性状基因，MKT 1和SSD 1的翻译调节因子的目标和相互作用。具体目标3这项工作是评估我们的数量性状基因在多个遗传背景的贡献。相关性：对数量性状的理解需要更好地理解转录/翻译调控、多效性、表型异质性和基因-环境相互作用。该项目的健康相关性是双重的。首先，人类大多数基于遗传的疾病负担本质上是定量的。因此，在一个简单的真核生物，如酵母数量性状的理解将有助于人类数量性状的理解。第二，S.酿酒酵母是一种新兴的机会致病菌，毒力是一种数量性状。因此，对数量性状的更好理解将有助于我们理解无害的嗜盐/嗜盐酵母如何成为机会致病菌。

DESCRIPTION (provided by applicant): Broad long-term objectives: The broad long-term objectives of this work are to improve our understanding of quantitative traits/genetics and genetic diversity, focusing on specific quantitative traits in S. cerevisiae. Improved understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions, all of which are addressed in this proposal. Specific Aims: Specific Aim 1 of this work is to identify in S. cerevisiae quantitative trait loci, quantitative trait genes/nucleotides controlling high temperature growth, mitochondrial genome maintenance, sporulation and flocculation. Specific Aim 2 of this work is to identify the targets and interactors of two of our high temperature growth quantitative trait genes, MKT1 and SSD1 that are translational regulators. Specific Aim 3 of this work is to assess the contributions of our quantitative trait genes in multiple genetic backgrounds. Relevance: The understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions. The health relatedness of this project is two-fold. First, most of the genetically based disease burden in humans is quantitative in nature. Therefore, an improved understanding of quantitative traits in a simple eukaryote such as yeast will aid the understanding of quantitative traits in humans. Second, S. cerevisiae is an emerging opportunistic pathogen and virulence is a quantitative trait. Therefore, an improved understanding of quantitative traits will aid our understanding of how a harmless commensal/saprophytic yeast can become an opportunistic pathogen.