ANALYSIS OF GENETICALLY & ENVIRONMENTALLY OVERLAPPING YEAST QUANTITATIVE TRAITS

描述（由申请人提供）：长期目标：本工作的长期目标是提高我们对酿酒酵母数量性状/遗传学和遗传多样性的理解，重点研究酿酒酵母的特定数量性状。提高对数量性状的理解需要更好地理解转录/翻译调控、多效性、表型异质性和基因-环境相互作用，所有这些都在本提案中得到解决。具体目的：本工作的目的1是鉴定酿酒酵母数量性状位点、控制高温生长、线粒体基因组维持、产孢和絮凝的数量性状基因/核苷酸。这项工作的具体目的2是确定两个高温生长数量性状基因MKT1和SSD1的靶点和相互作用因子，这两个基因是翻译调节因子。本工作的具体目的3是评估我们的数量性状基因在多种遗传背景下的贡献。相关性：对数量性状的理解需要更好地理解转录/翻译调控、多效性、表型异质性和基因-环境相互作用。这个项目与健康的关系是双重的。首先，人类中大多数基于遗传的疾病负担本质上是定量的。因此，提高对酵母等简单真核生物数量性状的理解将有助于了解人类的数量性状。其次，酿酒葡萄球菌是一种新兴的机会致病菌，毒力是一种数量性状。因此，提高对数量性状的理解将有助于我们理解无害的共生/腐生酵母如何成为机会致病菌。

DESCRIPTION (provided by applicant): Broad long-term objectives: The broad long-term objectives of this work are to improve our understanding of quantitative traits/genetics and genetic diversity, focusing on specific quantitative traits in S. cerevisiae. Improved understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions, all of which are addressed in this proposal. Specific Aims: Specific Aim 1 of this work is to identify in S. cerevisiae quantitative trait loci, quantitative trait genes/nucleotides controlling high temperature growth, mitochondrial genome maintenance, sporulation and flocculation. Specific Aim 2 of this work is to identify the targets and interactors of two of our high temperature growth quantitative trait genes, MKT1 and SSD1 that are translational regulators. Specific Aim 3 of this work is to assess the contributions of our quantitative trait genes in multiple genetic backgrounds. Relevance: The understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions. The health relatedness of this project is two-fold. First, most of the genetically based disease burden in humans is quantitative in nature. Therefore, an improved understanding of quantitative traits in a simple eukaryote such as yeast will aid the understanding of quantitative traits in humans. Second, S. cerevisiae is an emerging opportunistic pathogen and virulence is a quantitative trait. Therefore, an improved understanding of quantitative traits will aid our understanding of how a harmless commensal/saprophytic yeast can become an opportunistic pathogen.