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ANALYSIS OF GENETICALLY & ENVIRONMENTALLY OVERLAPPING YEAST QUANTITATIVE TRAITS

遗传分析

基本信息

批准号：
7894666
负责人：
John H. McCusker
金额：
$ 34.96万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2012-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Broad long-term objectives: The broad long-term objectives of this work are to improve our understanding of quantitative traits/genetics and genetic diversity, focusing on specific quantitative traits in S. cerevisiae. Improved understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions, all of which are addressed in this proposal. Specific Aims: Specific Aim 1 of this work is to identify in S. cerevisiae quantitative trait loci, quantitative trait genes/nucleotides controlling high temperature growth, mitochondrial genome maintenance, sporulation and flocculation. Specific Aim 2 of this work is to identify the targets and interactors of two of our high temperature growth quantitative trait genes, MKT1 and SSD1 that are translational regulators. Specific Aim 3 of this work is to assess the contributions of our quantitative trait genes in multiple genetic backgrounds. Relevance: The understanding of quantitative traits requires better understanding of transcriptional/translational regulation, pleiotropy, phenotypic heterogeneity, and gene-environment interactions. The health relatedness of this project is two-fold. First, most of the genetically based disease burden in humans is quantitative in nature. Therefore, an improved understanding of quantitative traits in a simple eukaryote such as yeast will aid the understanding of quantitative traits in humans. Second, S. cerevisiae is an emerging opportunistic pathogen and virulence is a quantitative trait. Therefore, an improved understanding of quantitative traits will aid our understanding of how a harmless commensal/saprophytic yeast can become an opportunistic pathogen.

描述（由申请人提供）：广泛的长期目标：这项工作的广泛的长期目标是提高我们对数量性状/遗传学和遗传多样性的理解，重点关注酿酒酵母的特定数量性状。提高对数量性状的理解需要更好地理解转录/翻译调控、多效性、表型异质性和基因-环境相互作用，所有这些都在本提案中得到解决。具体目标：这项工作的具体目标 1 是鉴定酿酒酵母数量性状基因座、控制高温生长、线粒体基因组维持、孢子形成和絮凝的数量性状基因/核苷酸。这项工作的具体目标 2 是确定我们的两个高温生长数量性状基因 MKT1 和 SSD1（翻译调节因子）的靶标和相互作用因子。这项工作的具体目标 3 是评估我们的数量性状基因在多种遗传背景中的贡献。相关性：对数量性状的理解需要更好地理解转录/翻译调控、多效性、表型异质性和基因-环境相互作用。该项目与健康的相关性有两个方面。首先，人类大多数基于遗传的疾病负担本质上是定量的。因此，更好地了解简单真核生物（如酵母）的数量性状将有助于了解人类的数量性状。其次，酿酒酵母是一种新兴的机会致病菌，毒力是一个数量性状。因此，更好地了解数量性状将有助于我们了解无害的共生/腐生酵母如何成为机会性病原体。