Structure & Function of Epididymis and Vas Deferens

结构

• 批准号: 7210587
• 负责人: KENNETH P ROBERTS
• 金额: $ 25.34万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-29 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7210587
• 关键词: Structure; Function; Epididymis; Vas; Deferens

DESCRIPTION (provided by applicant): Funds are requested to study the specific functions of the Crisp-1 protein. Crisp-1 is produced in the epididymis and associates with the sperm during transit through the organ. The high similarity to a known ryanodine receptor agonist suggests that Crisp-1 may have a role in ion channel regulation. Changes in calcium concentrations are required for sperm to become capacitated and undergo the acrosome reaction in preparation for fertilization. In vitro capacitation studies of rat sperm show that when Crisp-1 is present, sperm are prohibited from capacitating. The specific aims focus on determining the mechanisms involved in the inhibition of capacitation by Crisp-l, identification of the receptor for Crisp-1 on sperm, identification of the Crisp-1 active site, and investigation of the ability of other members of the Crisp family to mimic the actions of Crisp-1. Crisp-1 will also be tested for its ability to inhibit capacitation and the acrosome reaction in human sperm. Finally, potential actions of Crisp-1 on the egg, such as the ability to regulate cortical granule fusion, will be examined. The proposed studies will provide significant contributions to our understanding of sperm function and may also have potential application to male infertility and contraceptive development.

描述(由申请者提供)：需要资金来研究Crisp-1蛋白的特定功能。CRISP-1在附睾部产生，在精子通过该器官的过程中与精子联系在一起。与已知的Ryanodine受体激动剂高度相似，表明Crisp-1可能在离子通道调节中发挥作用。在准备受精的过程中，精子需要改变钙浓度才能获得能力并经历顶体反应。大鼠精子体外获能研究表明，当Crisp-1存在时，精子被禁止获能。这些研究的具体目的集中在确定Crisp-L抑制获能的机制、精子上Crisp-1受体的鉴定、Crisp-1活性部位的鉴定以及研究Crisp家族其他成员模仿Crisp-1的作用的能力。CRISP-1还将测试其抑制人类精子获能和顶体反应的能力。最后，我们将研究Crisp-1在卵子上的潜在作用，例如调节皮质颗粒融合的能力。建议的研究将为我们理解精子功能提供重要贡献，并可能对男性不育和避孕开发具有潜在的应用价值。

项目成果

Membrane glycoproteins from spermatozoa: partial characterization of an integral Mr = approximately 24,000 molecule from rat spermatozoa that is glycosylated during epididymal maturation.

来自精子的膜糖蛋白：来自大鼠精子的完整 Mr = 大约 24,000 个分子的部分表征，该分子在附睾成熟过程中被糖基化。

• DOI: 10.1095/biolreprod34.5.925
• 发表时间: 1986
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Hamilton,DW;Wenstrom,JC;Baker,JB
• 通讯作者: Baker,JB

The 26 kD protein recognized on rat cauda epididymal sperm by monoclonal antibody 4E9 has internal peptide sequence that is identical to the secreted form of epididymal protein E.

单克隆抗体 4E9 在大鼠附睾精子尾部识别的 26 kD 蛋白具有与附睾蛋白 E 的分泌形式相同的内部肽序列。

• DOI: 10.1002/(sici)1098-2795(199703)46:3<377::aid-mrd17>3.0.co;2-w
• 发表时间: 1997
• 期刊: Molecular reproduction and development.
• 作者: Xu,W;Ensrud,KM;Hamilton,DW
• 通讯作者: Hamilton,DW

Dolichol concentration and biosynthesis in rat testis and epididymis.

大鼠睾丸和附睾中的多醇浓度和生物合成。

• DOI: 10.1095/biolreprod23.5.1054
• 发表时间: 1980
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Wenstrom,JC;Hamilton,DW
• 通讯作者: Hamilton,DW

Immunocytochemical evidence for the specific localization of aldose reductase in rat Sertoli cells.

大鼠支持细胞中醛糖还原酶特异性定位的免疫细胞化学证据。

• DOI: 10.1095/biolreprod26.2.311
• 发表时间: 1982
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Ludvigson,MA;Waites,GM;Hamilton,DW
• 通讯作者: Hamilton,DW

An 18-kDa androgen-regulated protein that modifies galactosyltransferase activity is synthesized by the rat caput epididymidis, but has no structural similarity to rat milk alphalactalbumin.

一种可修饰半乳糖基转移酶活性的 18 kDa 雄激素调节蛋白由大鼠附睾合成，但与大鼠乳汁 α-乳白蛋白没有结构相似性。

• DOI: 10.1095/biolreprod43.3.497
• 发表时间: 1990
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Moore,A;Hall,L;Hamilton,DW
• 通讯作者: Hamilton,DW