Analyze mouse and cell culture models for PINK1 related Parkinson's disease

分析 PINK1 相关帕金森病的小鼠和细胞培养模型

• 批准号: 7259816
• 负责人: CHENJIAN LI
• 金额: $ 33.08万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-13 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7259816
• 关键词: ATP Synthesis Pathway; Bioenergetics; Biological Assay; Brain region; Calcium; Cell Line; Cessation of life; Chemicals; Complex; Cultured Cells; Data; Disease; Disruption; Dopamine; Dopaminergic Cell; Etiology; Failure; Figs - dietary; Free Radicals; Functional disorder; Generations; Genes; Genetic; Genome; Homeostasis; In Vitro; Investigation; Knockout Mice; Lead; Left; Localized; Membrane Potentials; Mitochondria; Modeling; Molecular; Mus; Mutation; Neurodegenerative Disorders; Neuronal Dysfunction; Neurons; Nuclear; Outcome; Oxidative Stress; Oxygen Consumption; PTEN gene; Parkinson Disease; Parkinsonian Disorders; Pathogenesis; Pathology; Patients; Peptide Signal Sequences; Phenotype; Phosphorylation; Phosphotransferases; Physiological; Population Study; Preclinical Drug Evaluation; Production; Proteins; Reagent; Research Personnel; Respiratory Chain; Role; Substantia nigra structure; Testing; dopaminergic neuron; early onset; loss of function; mitochondrial dysfunction; mitochondrial membrane; motor deficit; mouse model; mutant; neurochemistry; pars compacta; programs; therapeutic target; tool

DESCRIPTION (provided by applicant): Mitochondria dysfunction and oxidative stress have been suggested to be associated with many neurodegenerative diseases, including Parkinson's Disease (PD). The most direct CAUSALITY evidence of mitochondria dysfunction to PD pathogenesis arises from the newly identified PINK1, a disease gene of familial, recessive early-onset PD. PINK1 protein is a nuclear genome encoded protein with a mitochondria targeting signal peptide and a conserved kinase domain, however, its normal functions and roles in pathogenesis remain to be elucidated. The mutations in PINK1-PD patients are causing the PINK1 proteins to be truncated or most likely functionally inactive. HYPOTHESIS: Our overall hypothesis is that PINK1-PD pathology is caused by disruption of mitochondria functions because the mutant PINK1 protein fails to phosphorylate its normal substrates. To test this hypothesis, we have generated genetic PINK1-/- knockout mice and other valuable reagents, and we propose the following specific aims. SPECIFIC AIM 1: we will analyze whether PINK1-/- mice develop progressive motor deficits and neuronal pathology in substantia nigra and other brain regions. SPECIFIC AIM 2: Subsequently, but not dependent on the Aim 1 outcome, we will investigate mitochondria dysfunction in PINK1 -/- mice and in PINK1-/- dopaminergic cell lines. SPECIFIC AIM 3: Subsequently, but not dependent on the Aim1 outcome, we will identify and confirm the substrates of PINK1 kinase. SIGNIFICANCE: Collectively, the generation and characterization of the PINK1 knockout mice as well as the dopaminergic PINK1-/- cell lines, the investigation of the mitochondria dysfunction caused by PINK1 mutations, and the identification of PINK1 kinase substrates are critical for understanding of the molecular mechanisms of PINK1-PD, for identifying potential therapeutic targets, and for drug screening and testing.

描述（由申请人提供）：线粒体功能障碍和氧化应激已被认为与许多神经退行性疾病相关，包括帕金森病（PD）。线粒体功能障碍与PD发病机制最直接的因果关系证据来自新发现的PINK 1，一种家族性、隐性早发性PD的疾病基因。PINK 1蛋白是一种核基因组编码的蛋白质，具有线粒体靶向信号肽和保守的激酶结构域，但其正常功能和在发病机制中的作用尚不清楚。PINK 1-PD患者的突变导致PINK 1蛋白被截短或很可能功能失活。假设：我们的总体假设是，PINK 1-PD病理是由线粒体功能破坏引起的，因为突变的PINK 1蛋白不能磷酸化其正常底物。为了验证这一假设，我们已经产生了基因PINK 1-/-敲除小鼠和其他有价值的试剂，我们提出了以下具体目标。具体目标1：我们将分析PINK 1-/-小鼠是否在黑质和其他脑区域发展进行性运动缺陷和神经元病理学。具体目标2：随后，但不依赖于目标1的结果，我们将研究PINK 1-/-小鼠和PINK 1-/-多巴胺能细胞系的线粒体功能障碍。具体目标3：随后，但不依赖于Aim 1的结果，我们将确定和确认PINK 1激酶的底物。重要性：总的来说，PINK 1基因敲除小鼠以及多巴胺能PINK 1-/-细胞系的产生和表征、PINK 1突变引起的线粒体功能障碍的研究以及PINK 1激酶底物的鉴定对于理解PINK 1-PD的分子机制、鉴定潜在的治疗靶点以及药物筛选和测试至关重要。