MicroRNA networks in skin inflammation

皮肤炎症中的 MicroRNA 网络

• 批准号: 2894815
• 金额: --
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2894815
• 关键词: MicroRNA; networks; skin; inflammation

The project will explore how microRNAs contribute to skin immune and stromal cell function and cell-to-cell communication using advanced in vitro models, cutting-edge imaging, immunological, and molecular cell biology techniques, and by analysing clinical samples. The project will focus on acne, the most common inflammatory dermatosis seen worldwide. Driven by aberrant inflammation, scarring can occur in up to 95% of people who develop the condition. Dissecting how post-transcriptional networks contribute to pathogenic skin inflammation will be the main topic of this project. Importantly, the mechanisms explored here will be of high relevance to skin inflammation beyond the context of acne.Building on our recently published work, which identified acne-associated microRNAs (Ghumra et al, 2021), the project will develop and validate 2-D and 3-D cellular models to assess their mechanism of action with a particular focus on neutrophil-endothelium interactions. We will also assess tissue cellular origin and levels and circulating levels of acne-associated microRNAs in clinical samples before and after treatment. Mechanistic and clinical studies will be integrated to gain insight into how microRNAs can inform current treatments or be used as therapeutic targets.

该项目将探索microRNA如何有助于皮肤免疫和基质细胞功能以及细胞与细胞之间的通信，使用先进的体外模型，尖端成像，免疫学和分子细胞生物学技术，并通过分析临床样本。该项目将重点关注痤疮，这是世界上最常见的炎症性皮肤病。由于异常炎症的驱动，高达95%的人会出现疤痕。剖析转录后网络如何有助于致病性皮肤炎症将是这个项目的主要议题。重要的是，这里探索的机制将与痤疮背景之外的皮肤炎症高度相关。基于我们最近发表的工作，确定了痤疮相关的microRNA（Ghumra et al，2021），该项目将开发和验证二维和三维细胞模型，以评估其作用机制，特别关注嗜中性粒细胞-内皮细胞相互作用。我们还将评估治疗前后临床样本中痤疮相关microRNA的组织细胞来源和水平以及循环水平。机制和临床研究将被整合，以深入了解microRNA如何为当前的治疗提供信息或用作治疗靶点。